Molecular cloning, characterization and expression analysis of MADS-box genes associated with reproductive development in Momordica dioica Roxb.

Abstract

The repertoire and functions of MADS-box family transcription factors (TFs) largely remains unexplored with respect to floral organogenesis of Roxb. Degenerative PCR followed by rapid amplification of cDNA ends was employed in the present study to clone and characterize 17 MADS-box genes (designated as to ) from the floral buds of The cloned genes were clustered into three subgroups (11 MIKC, 4 MIKC* and 2 Mα) based on phylogenetic relationships with the MADS-box genes from and. Southern hybridization showed that all the isolated genes were represented by single copy locus in the genome. Gene structure analysis revealed 1-8 exons in-box genes with the number of exons in MIKC greatly exceeding from that in M-type genes. Motif elicitation of the -box genes indicated the presence of additional domains with MIKC type, suggesting that they had more complex structures. Expression analysis of genes in six transcriptome suggested that, 11 MIKC-type genes are associated with floral homeotic functions, 4 MIKC*-type genes ( to ) controlled the growth of male gametophyte, while the two-type genes ( and ) played significant role in female gametogenesis and seed development. Overall, these are the first set of MADS-box genes from exhibiting a differential expression pattern during floral development. The results from this study will provide valuable information for further functional studies of candidate MADS-box genes in the sexual dimorphism of this economically important dioecious cucurbit.

Genome-wide analysis of MADS-box gene family in kiwifruit (Actinidia chinensis var. chinensis) and their potential role in floral sex differentiation

“…For example, LcMADS51 may be involved in the formation of litchi carpel, and six MADS-box genes including LcMADS42/46/47/75/93/100 may play a role in Frontiers in Genetics frontiersin.org stamen development (Guan et al, 2021). In Momordica dioica Roxb, MdMADS12/13/14/15 regulate male gametophyte development, while MdMADS16 and MdMADS17 play important roles in female gametophyte formation and seed development (Mohanty and Joshi, 2018). In Cunninghamia lanceolata, MADS-box genes including GGM7, SVP, and AGL15 are candidate genes for sex-specific development of cones due to their specific expression in female/male cones (Wang et al, 2020).…”

Comparative Transcriptome Reveals the Genes’ Adaption to Herkogamy of Lumnitzera littorea (Jack) Voigt

“…MADS-box proteins are generally divided into types I and II. Type I is categorized into Mα, Mβ, Mγ, and Mδ clades and type II is classified into MIKC c and MIKC ∗ ( Mohanty and Joshi, 2018 ). Several type I MADS box TFs are shown to be involved in reproductive development in Arabidopsis ( Luo et al, 2008 ).…”

“…MADS-box transcription factors play crucial roles in floral organ formation, embryo and reproductive development and flowering time control ( Angenent, 1995 ; Moon et al, 2003 ; Arora et al, 2007 ; Irish, 2010 ; Mohanty and Joshi, 2018 ; Zhang et al, 2018 ). Extensive studies of Arabidopsis mutants show several genetic models of floral organ formation ( Coen and Meyerowitz, 1991 ; Theissen et al, 2016 ).…”

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“…MADS-box transcription factors play crucial roles in floral organ formation, embryo and reproductive development and flowering time control (Angenent, 1995;Moon et al, 2003;Arora et al, 2007;Irish, 2010;Mohanty and Joshi, 2018;Zhang et al, 2018). Extensive studies of Arabidopsis mutants show several genetic models of floral organ formation (Coen and Meyerowitz, 1991;Theissen et al, 2016).…”