Molecular cloning, characterization and expression analysis of HSP60, HSP70 and HSP90 in the golden apple snail, Pomacea canaliculata

Xu, Yipeng; Zheng, Guowan; Dong, Shao-Jing; Liu, Guangfu; Yu, Xiaoping

doi:10.1016/j.fsi.2014.10.013

Cited by 50 publications

(15 citation statements)

References 49 publications

(41 reference statements)

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“…This suggests that RpHSP60 may be involved in the adaptive response to heat stress by maintaining correct protein folding (Oksala et al 2014). In contrast, cold treatment had little effect on RpHSP60 expression in the current study, in accordance with a previous study of HSP60 expression levels in the gills of P. canaliculata (Xu et al 2014). This may be because low temperature reduced ROSproduction rates in R. philippinarum, in line with the general perception of the effects of body temperature on biochemical and enzymatically catalyzed cellular reactions (Abele and Puntarulo 2004).…”

Section: Discussionsupporting

confidence: 92%

“…HSP60 genes have been studied in some aquatic invertebrates, including sea anemones (Anemonia viridis) (Choresh et al 2001;Choresh et al 2004), white shrimp (Litopenaeus vannamei) (Zhou et al 2010), crabs (Portunus trituberculatus) (Xu and Qin 2012), and the mud crab (Scylla paramamosain) (Yang et al 2013). However, the stress response in mollusks has been relatively poorly studied, except the zebra mussel (Dreissena polymorpha) (Clayton et al 2000), golden apple snail (Pomacea canaliculata) (Xu et al 2014), Japanese scallop (Mizuhopecten yessoensis) (Fandong et al 2015), and Pacific oyster (Crassostrea gigas) (Zhu et al 2016), and information about the role of HSP60 in the stress response in mollusks is therefore limited.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characteristics of a novel HSP60 gene and its differential expression in Manila clams (Ruditapes philippinarum) under thermal and hypotonic stress

Ding

Yang

et al. 2018

Cell Stress and Chaperones

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The Manila clam Ruditapes philippinarum inhabits the intertidal zone and must therefore tolerate broad fluctuations in water temperature and salinity. Heat shock protein 60 (HSP60) is an evolutionarily conserved, multi-functional protein that plays a significant role in protecting organisms from harmful stress conditions. We cloned the R. philippinarum HSP60 (RpHSP60) gene and analyzed its transcriptional responses to thermal and low-salinity stresses. The complete sequence of RpHSP60 cDNA was 1777 nucleotides, containing a 1728-bp open reading frame encoding a polypeptide of 576-amino acids, with a calculated molecular mass of 61.25 kDa and predicted isoelectric point of 5.08. Comparisons of amino acid sequences and three-dimensional structures of HSP60 revealed that RpHSP60 was highly conserved in the signature HSP60-family domains. RpHSP60 mRNA was detected in all the tested tissues of R. philippinarum, with the highest expression levels in hemocytes. We measured RpHSP60 mRNA levels in the gills under thermal and low-salinity stresses using quantitative real-time reverse transcription-polymerase chain reaction. Following the thermal challenge, RpHSP60 mRNA was significantly upregulated at 6 h, and then progressively downregulated under high-temperature stress (30 °C), while only slight fluctuations were observed under low-temperature stress (-1 °C). Under low-salinity (17 ppt) stress, RpHSP60 mRNA levels were significantly increased at 3, 72, and 96 h (P < 0.05). These results suggest that HSP60 of R. philippinarum may play important roles in responding to high-temperature and low-salinity stresses.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Molecular characteristics of a novel HSP60 gene and its differential expression in Manila clams (Ruditapes philippinarum) under thermal and hypotonic stress

Ding

Yang

et al. 2018

Cell Stress and Chaperones

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“…Surprisingly, the brain showed a significantly decreased after cold treatment (8.5°C), while other tissues had no significant difference. These results were in agreement with HSP60 expression levels in Pomacea canaliculata (Xu et al 2014b). The AcaHSP60 expression also showed tissue specificity for the temperature treatment.…”

Section: Gene Expression Analysissupporting

confidence: 87%

HSP60 expression profile under different extreme temperature stress in albino northern snakehead, Channa argus

Zhou

Xie

Wang

et al. 2018

Cell Stress and Chaperones

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The great albino northern snakehead, Channa argus, is one of the most important economical fish in China. In the present study, cDNA encoding heat shock protein 60 (HSP60) was cloned and characterized. The cDNA was 2462 bp, containing an open reading frame (ORF) encoding a 575-amino-acids polypeptide of 61.10 kDa (theoretical isoelectric point [pI]: 5.66). BLAST analysis showed that AcaHSP60 was highly similar with other HSP60s, and three conserved amino acid blocks and characteristic motifs or domains defined as HSP60 protein family signatures. Genomic DNA analysis showed that AcaHSP60 had ten exons in the coding region (from 94 to 336 bp). Changes in AcaHSP60 gene expression profiles in albino C. argus experimentally exposed to different temperature stress (8.5, 26, and 37 °C) was investigated. Quantitative real-time PCR and western blot analysis revealed that tissue-specific AcaHSP60 expressions were in the spleen, muscle, liver, kidney, heart and brain. Expression was highly significantly stimulated after heat shock (37 °C), but showed no significant differences after cold treatment (8.5 °C) except in the brain. In summary, these results showed that AcaHSP60 was significantly tissue specific and indicate that AcaHSP60 expression might be sensitive to thermal resistance in albino C. argus.

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“…The other two novel genes belong to the Hsp60 and Hsp70 families. Hsp60 is a protein involved in the stress response in the mitochondria; one study describes its interaction with Hsp10 in other mollusks 48 . GRP78 is a chaperone that belongs to the Hsp70 family; it participates in the unfolded protein response (UBR) in the endoplasmic reticulum and forces the unfolded proteins to refold or degrade using cellular degradation mechanisms 38 .…”

Section: Discussionmentioning

confidence: 99%

Endosulfan exposure alters transcription of genes involved in the detoxification and stress responses in Physella acuta

Alonso-Trujillo

Muñiz-González

Martínez-Guitarte

2020

Sci Rep

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Endosulfan is a persistent pesticide that has been in use for more than five decades. During this time, it has contaminated soil, air, and water reservoirs worldwide. It is extremely toxic and harmful to beneficial non-target invertebrates, aquatic life, and even humans upon consumption, which is one of the many dangers of this pesticide since it biomagnifies in the food chain. The effects of three endosulfan concentrations (1, 10, and 100 µg/L) on the freshwater snail Physella acuta, an invasive cosmopolitan species, were examined over a week-long exposure period. Alterations in the expression of ten genes related to stress and xenobiotic detoxification were measured against the endogenous controls rpL10 and GAPDH by Real-Time polymerase chain reaction. Four genes are described here for the first time in this species, namely Hsp60, Grp78, GSTk1, and GSTm1. The rest of genes were Hsp90, sHsp16.6, cyp2u1, cyp3a7, cyp4f22, and MRP1. cyp2u1, sHsp16.6, and Grp78 expression were all altered by endosulfan. These results suggest a low pesticide concentration activates the acute response in P. acuta by affecting detoxification and stress responses and alter endoplasmic reticulum function and lipid metabolism. Furthermore, the newly identified genes extend the number of processes and cellular locations that can be analyzed in this organism. Endosulfan, also known as Thiodan or Thionex, is a polychlorinated compound used as a pesticide or acaricide. It has been in use since the 1950s in China, the European Union, Australia, Mexico, the United States, and India 1 for crops including maize, soybeans, tomatoes, and cotton 2. It is particularly effective in removing aphids, fruit worms, beetles, leafhoppers, moth larvae, and whiteflies 3. However, its highly toxic properties can potentially harm humans and wildlife 4,5. Further, due to its semi-volatility, endosulfan is often found thousands of kilometers away from its intended area of action in soil, water reservoirs, sediments, and even Arctic and Antarctic lakes. These findings prove its persistent character; indeed, it is listed as a persistent organic pollutant in the atmosphere, soil, and water by the Stockholm Convention 6. For this reason, it has been banned in over 80 nations. However, one of the main problems with pesticides as endosulfan is the fact that they are persistent and can be dispersed from air to water bodies. The environmental presence of endosulfan has been evaluated by Mohamed et al., 2019 7 showing values from 0.036 μg/L to 62.3 μg/L in water bodies. Indeed, it is an extremely toxic and harmful compound to beneficial non-target invertebrates living in aquatic media 8-10. Endosulfan is commercially available as a mixture of its isomers, α and β, but higher environmental concentrations correspond to α-endosulfan, since β-endosulfan is naturally converted into α-endosulfan in a slow way due to its higher stability 3. Although they share similar chemical and physical properties, under aerobic conditions the β-endosulfan half-life is approximately 28 days, ...

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Molecular cloning, characterization and expression analysis of HSP60, HSP70 and HSP90 in the golden apple snail, Pomacea canaliculata

Cited by 50 publications

References 49 publications

Molecular characteristics of a novel HSP60 gene and its differential expression in Manila clams (Ruditapes philippinarum) under thermal and hypotonic stress

Molecular characteristics of a novel HSP60 gene and its differential expression in Manila clams (Ruditapes philippinarum) under thermal and hypotonic stress

HSP60 expression profile under different extreme temperature stress in albino northern snakehead, Channa argus

Endosulfan exposure alters transcription of genes involved in the detoxification and stress responses in Physella acuta

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