Molecular cloning, characterization and expression analysis of a C-type lectin (AJCTL) from the sea cucumber Apostichopus japonicus

Han, Lulu; Yuan, Zhe; Dahms‬, Hans-Uwe; Li, Qiuying; Zhang, Qiao-Zhi; Wu, Rongjie; Tan, Jing-He; Zou, Xiangyang; Lin, Hui

doi:10.1016/j.imlet.2011.12.004

Cited by 22 publications

(11 citation statements)

References 36 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The wide distribution of AjCTL-1 in sea cucumber was consistent with previous finding about CTLs in other marine invertebrates [45,54], and indicative of its critical role in the immune response against invading microorganisms. Despite the wide distribution in different tissues, the mRNA expression level of AjCTL-1 was highest in gonad, while another sea cucumber CTL, AJCTL, showed the highest expression level in the longitudinal muscle [38]. The different expression profiles of AjCTL-1 and AJCTL in tissues indicate these two CTLs may have different functions in the innate immunity of A. japonicus.…”

Section: Discussionmentioning

confidence: 92%

“…A growing number of CTLs have recently been discovered from marine invertebrates, especially crustaceans and molluscs, including those from F. chinensis [16,21,46,47], L. vannamei [15,19,45,48], Penaeus monodon [49,50], C. farreri [18,23,24,26], A. irradians [22,25] and Crassostrea gigas [51], Crassostrea virginica [52], and Haliotis discus discus [17]. Relatively little information is available on CTLs in echinoderms, and a handful of sea cucumber CTLs have been reported, such as MBL-C (Cucumaria japonica) [53], SJL-I (S. japonicus) [35,36], MBL-AJ and AJCTL (A. japonicus) [37,38]. Although their mRNA expression patterns and biological characteristics have been described, the function of sea cucumber CTLs remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…The third group without any treatment served as the blank control [38]. At 3, 6, 12, 24 and 48 h post-challenge (hpc), four sea cucumbers were taken randomly from each group and sacrificed to harvest the coelomocytes.…”

Section: Mrna Expression Analysis After Bacterial Challengementioning

confidence: 99%

“…Later, MBL-AJ was isolated from the coelomic plasma of A. japonicus [37]. More recently, AJCTL has also been identified from A. japonicus and its expression profile was characterized [37,38]. Most of these reports have focused on the purification of CTLs, characterization of amino acid sequences and their expression pattern in echinoderms.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

View full text Add to dashboard Cite

a b s t r a c t C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other Ctype lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca 2þ -binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different upregulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca 2þ -dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes.

show abstract

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Mrna Expression Analysis After Bacterial Challengementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

View full text Add to dashboard Cite

show abstract

“…Vibrio, Pseudomonas and even spherical virus had been isolated or identified from different sources of diseased sea cucumber [2,5e7]. Regarding to immune defense of sea cucumber, various effectors like lectin [8], lysozyme [9,10] and complement component 3 [11] had been isolated and characterized at mRNA and protein levels. However, interpretation their interaction between the host and pathogen was not concerned to our knowledge.…”

Section: Introductionmentioning

confidence: 99%

Characterization of skin ulceration syndrome associated microRNAs in sea cucumber Apostichopus japonicus by deep sequencing

Feng

Qiu

et al. 2012

Fish & Shellfish Immunology

107

View full text Add to dashboard Cite

Identification and characterization of two novel C‐type lectins from the larvae of housefly, Musca domestica L.

Zhou

Fang

Zheng

et al. 2018

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Lectins and antimicrobial peptides (AMPs) are widely distributed in various insects and play crucial roles in primary host defense against pathogenic microorganisms. Two AMPs (cecropin and attacin) have been identified and characterized in the larvae of housefly. In this study, two novel C-type lectins (CTLs) were obtained from Musca domestica, while their agglutinating and antiviral properties were evaluated. Real-time PCR analysis showed that the mRNA levels of four immune genes (MdCTL1, MdCTL2, Cecropin, and Attacin) from M. domestica were significantly upregulated after injection with killed Gram-negative Escherichia coli. Moreover, purified MdCTL1-2 proteins can agglutinate E. coli and Staphylococcus aureus in the presence of calcium ions, suggesting their immune function is Ca dependent. Sequence analysis indicated that typical WND and QPD motifs were found in the Ca -binding site 2 of carbohydrate recognition domain from MdCTL1-2, which was consistent with their agglutinating activities. Subsequently, antiviral experiments indicated that MdCTL1-2 proteins could significantly reduce the infection rate of Spodoptera frugiperda 9 cells by the baculovirus Autographa californica multicapsid nucleopolyhedrovirus, indicating they might play important roles in insect innate immunity against microbial pathogens. In addition, MdCTL1-2 proteins could effectively inhibit the replication of influenza H N virus, which was similar to the effect of ribavirin. These results suggested that two novel CTLs could be considered a promising drug candidate for the treatment of influenza. Moreover, it is believed that the discovery of the CTLs with antiviral effects in M. domestica will improve our understanding of the molecular mechanism of insect immune response against viruses.

show abstract

Molecular cloning, characterization and expression analysis of a C-type lectin (AJCTL) from the sea cucumber Apostichopus japonicus

Cited by 22 publications

References 36 publications

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Characterization of skin ulceration syndrome associated microRNAs in sea cucumber Apostichopus japonicus by deep sequencing

Identification and characterization of two novel C‐type lectins from the larvae of housefly, Musca domestica L.

Contact Info

Product

Resources

About