Molecular cloning and transcriptional activation of lysozyme‐encoding cDNAs in the mosquito <i>Aedes aegypti</i>

Bedoya, R. J. Ursic; Mitzey, Ann M.; Obraztsova, M.; Lowenberger, Carl

doi:10.1111/j.1365-2583.2004.00534.x

Cited by 40 publications

(20 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lysozymes, present in many organisms, cleave the β-1, 4-glycosidic linkage between N-acetylmuramic acid and N-acetylglucosamine found in certain bacterial cell walls [42]. Their functions have been widely described in species that ingest or harbour bacteria throughout their life cycles, such as M. domestica and D. melanogaster , and also characterized as digestive enzymes [42]–[45].…”

Section: Resultsmentioning

confidence: 99%

“…Their functions have been widely described in species that ingest or harbour bacteria throughout their life cycles, such as M. domestica and D. melanogaster , and also characterized as digestive enzymes [42]–[45]. Other reports have suggested a major role of lysozymes in immune responses to pathogens [42], [46]. We found that two of the four lysozyme unigenes up-regulated and two other down-regulated post-infection, suggesting Musca lysozymes may function as defense molecules as well as digestive enzymes.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptional Response of Musca domestica Larvae to Bacterial Infection

Tang

Yang

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

The house fly Musca domestica, a cosmopolitan dipteran insect, is a significant vector for human and animal bacterial pathogens, but little is known about its immune response to these pathogens. To address this issue, we inoculated the larvae with a mixture of Escherichia coli and Staphylococcus aureus and profiled the transcriptome 6, 24, and 48 h thereafter. Many genes known to controlling innate immunity in insects were induced following infection, including genes encoding pattern recognition proteins (PGRPs), various components of the Toll and IMD signaling pathways and of the proPO-activating and redox systems, and multiple antimicrobial peptides. Interestingly, we also uncovered a large set of novel immune response genes including two broad-spectrum antimicrobial peptides (muscin and domesticin), which might have evolved to adapt to house-fly's unique ecological environments. Finally, genes mediating oxidative phosphorylation were repressed at 48 h post-infection, suggesting disruption of energy homeostasis and mitochondrial function at the late stages of infection. Collectively, our data reveal dynamic changes in gene expression following bacterial infection in the house fly, paving the way for future in-depth analysis of M. domestica's immune system.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Transcriptional Response of Musca domestica Larvae to Bacterial Infection

Tang

Yang

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…A number of studies have shown an increased lysozyme expression in the mosquitoes after challenge with bacteria (Fujimoto et al, 2001; Lee and Brey, 1995; Sun et al, 1991; Gao and Fallon, 2000; Hernandez et al, 2003; Bedoya et al, 2005; Kajla et al, 2010). In blood fed mosquitoes the bacterial population increases significantly within the midgut (Beier, 1998, DeMaio et al, 1996; Pumpuni et al, 1993, 1996; Bedoya et al, 2005). Recently Dong et al (2009) showed that the presence of gut microbiota indirectly impacted the susceptibility of the mosquitoes to become infected with malaria parasites apparently via modulation of the basal immune response.…”

Section: Discussionmentioning

confidence: 99%

“…Because mosquito lysozymes belong to an expanded gene family (Li et al, 2005; Bedoya et al, 2005), we are also interested in investigating the biological functions of each member of the group. As a first step towards that goal, we analyzed the expression profiles for AdLys c-1 across stages and tissues of An.…”

Section: Discussionmentioning

confidence: 99%

Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development

Lapcharoen

Komalamisra

Rongsriyam

et al. 2012

Developmental & Comparative Immunology

View full text Add to dashboard Cite

A cDNA encoding a lysozyme was obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) from females of the malaria vector Anopheles dirus A (Diptera: Culicidae). The 623 bp lysozyme (AdLys c-1) cDNA encodes the 120 amino acid mature protein with a predicted molecular mass of 13.4 kDa and theoretical pI of 8.45. Six cysteine residues and a potential calcium binding motif that are present in AdLys c-1 are highly conserved relative to those of c-type lysozymes found in other insects. RT-PCR analysis of the AdLys c-1 transcript revealed its presence at high levels in the salivary glands both in larval and adult stages and in the larval caecum. dsRNA mediated gene knockdown experiments were conducted to examine the potential role of this lysozyme during Plasmodium berghei infection. Silencing of AdLys c-1 resulted in a significant reduction in the number of oocysts as compared to control dsGFP injected mosquitoes.

show abstract

“…C- and i-type lysozymes have been reported in several insect orders, including Diptera, Lepidoptera, Orthoptera, Isoptera, and Hemiptera 12-14. For example, thirteen, eight, and six c-type lysozyme genes are identified in the genome of Drosophila melanogaster 15, Anopheles gambiae 16, and Aedes agypti 17, respectively. Five of eighteen lysozyme genes in D. melanogaster encode i-type lysozymes 15.…”

Section: Introductionmentioning

confidence: 99%

Identification and Expression Profile Analysis of Antimicrobial Peptide/Protein in Asian Corn Borer, Ostrinia furnacalis (Guenée)

Zhang¹,

Zhou²,

Cheng³

et al. 2013

Int. J. Biol. Sci.

View full text Add to dashboard Cite

Antimicrobial peptides/proteins (AMPs) are a group of immune proteins that exhibit strong antibiotic properties against numerous infectious bacterial strains. They are evolutionarily conserved and present in every kingdom and phylum, ranging from prokaryotes to humans. We analyzed the transciptome from the larvae of Asian corn borer, Ostrinia furnacalis (Guenée), and identified several putative AMP transcripts, OfgLys5, OfgLys6, OfgLys10, OfgAtt, and OfgIID. OfgLys5, OfgLys6, and OfgLys10 are all highly homologous with c-type lysozymes, and OfgAtt shows significant identities with Lepidoptera attacin. The amino acid sequence of OfgLys5 and OfgLys6 possessed all conserved features critical for fundamental structure and function of c-type lysozyme, including the two catalytic sites, Glu32 and Asp50. OfgAtt is a typical glycine-rich protein. The antimicrobial activity of O. furnacalis hemolymph increased significantly after injection with Escherichia coli, Micrococcus luteus, or Beauveria bassiana. OfgAtt, IDD, and Lys6 are expressed at low level prior to the challenge, but strongly induced against Gram-positive and negative bacteria, and fungi. Under the same inducement conditions, the transcripts of these three genes elevated most when fifth instar larvae were injected. Therefore, O. furnacalis larvae are induced to produce antimicrobial materials in the hemolymph after the infection, and increase of lysozyme and attacin may contribute to the antimicrobial activity.

show abstract

Molecular cloning and transcriptional activation of lysozyme‐encoding cDNAs in the mosquito Aedes aegypti

Cited by 40 publications

References 19 publications

Transcriptional Response of Musca domestica Larvae to Bacterial Infection

Transcriptional Response of Musca domestica Larvae to Bacterial Infection

Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development

Identification and Expression Profile Analysis of Antimicrobial Peptide/Protein in Asian Corn Borer, Ostrinia furnacalis (Guenée)

Contact Info

Product

Resources

About