Molecular cloning and tissue distribution of lipoprotein lipase full-length cDNA from Pengze crucian carp (Carassius auratus var. Pengze)

Cheng, Hanliang; Wang, Xin; Peng, Yong-xing; Meng, Xianhong; Sun, Sijia; Shi, Xiaodong

doi:10.1016/j.cbpb.2009.02.006

Cited by 26 publications

(15 citation statements)

References 22 publications

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“…Previous studies showed that LPL gene was not expressed in liver for adult mammal or birds (Sato, Seol & Kamada 2010), but expressed in embryonic liver, or re-expressed in adult liver when suffering from partial hepatectomy or treated with fibric acid derivatives (fenofibrate) in rat (Rattus norvegicus) (Burgaya, Peinado, Vilaro, Llobrea & Ramirez 1989;Peinado-Onsurbe, Staels, Deeb, Ramirez, Llobera & Auwerx 1992;. In teleost, LPL was expressed in various tissues, such as liver, muscle, heart and stomach (Lindberg & Olivecrona 2002;Saera-Vila et al 2005;Cheng et al 2009;Zheng et al 2013). In our results, LPL was detected in all the examined nine tissues and expressed highly in liver.…”

Section: Figurementioning

confidence: 99%

“…As a rate-limiting enzyme, the key function of LPL was to hydrolyse circulating chylomicrons (CM, major ingredient is triacylglycerol) and very-low-density lipoproteins (VLDL) into free fatty acids (FFAs), which as a ready substrate for b-oxidation to provide power during the process of lipid metabolism (Mead, Irvine & Ramji 2002). There was a considerable evidence showing that in fish the expression levels of LPL were tissue-specific (Cheng, Wang, Peng, Meng, Sun & Shi 2009;Cheng, Sun, Peng, Shi, Shen, Meng & Dong 2010;Li, Jiang, Qian, Xu & Liu 2013), mostly expressed in adipose tissue, heart and liver (Saera-Vila, Calduch-Giner, G omez-Requeni, M edale, Kaushik & P erez-S anchez 2005; Oku, Koizumi, Okumura, Kobayashi & Umino 2006;Zheng, Luo, Zhu, Tan, Chen, Sun & Hu 2013). Moreover, it was reported that the activity and expression of LPL can be regulated by hormones (insulin, triiodothyronine) (Oku et al 2006; Albalat, Saera-Vila, Capilla, Guti errez, P erez-S anchez & Navarro 2007), fasting (Han et al 2011;Tian et al 2013), re-feeding (Oku et al 2006) and feed compensation .…”

Section: Introductionmentioning

confidence: 99%

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Changes in activities and mRNA expression of lipoprotein lipase and fatty acid synthetase in large yellow croaker,Larimichthys crocea(Richardson), during fasting

et al. 2016

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Over‐winter fasting and man‐made food deprivation to increase meat quality are common in the process of large yellow croaker (Larimichthys crocea) aquaculture. This study aimed to determine the changes in lipoprotein lipase (LPL) and fatty acid synthetase (FAS) activities and mRNA expressions, and the relationships between these changes and fat content in large yellow croaker liver and muscle tissues during fasting. A total of 2933 bp LPL cDNA, including an open reading frame of 1533 bp encoding 510 amino acids, and a 1233 bp fragment of FAS cDNA coding 411 amino acids were cloned. Expressions of both genes were ubiquitous. During a 35‐day fasting period, the hepatosomatic index and fat content in muscle and liver were significantly decreased (P < 0.05). mRNA levels of LPL increased significantly during the fasting period except the first 3 days, and FAS mRNA levels decreased significantly in both muscle and liver (P < 0.05) although there were some fluctuations. Muscle and liver LPL activities were significantly higher following fasting for 7 days, decreased to the initial value following fasting for 14 days, and elevating significantly afterwards (P < 0.05). FAS activities in muscle and liver maintained a significantly decreasing trend during the short‐term fasting (P < 0.05) and kept obviously rising thereafter (P < 0.05). Activities and mRNA levels of both LPL and FAS were not always consistent, which implied that both pre‐translational and post‐translational regulations existed during fasting. Our results suggest that the reasonable fasting time is 21 days before harvesting when fat content decreased significantly.

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Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Changes in activities and mRNA expression of lipoprotein lipase and fatty acid synthetase in large yellow croaker,Larimichthys crocea(Richardson), during fasting

et al. 2016

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“…In the present study, the mRNA levels of lpl in liver were 38.6‐, 22.2‐, 40.7‐, 206.2‐, 39.7‐, and 741.1‐fold higher than those in spleen, muscle, intestine, brain, heart, and gill, respectively ( p <0.01). Similarly, substantial amounts of lpl transcripts have been detected in the liver of O. mykiss , Pagrus major , and C. auratus , indicating that lpl gene is mainly expressed in tissues with the high lipid demand for either oxidation or storage. On the other hand, Paredes et al pointed out that the expression pattern of lpl showed clear nocturnal rhythms and their peaks of expression were located at the end of the dark phase.…”

Section: Resultsmentioning

confidence: 99%

“…The current study extends our understanding of lipid metabolism in different tissues at the transcriptional level, which will facilitate further studies on the regulation of lipid metabolism at the molecular level for the fish species. Its production of malonyl-CoA is the committed step in the biosynthesis of long-chain fatty acids Lipogenesis [46] accb Its malonyl-CoA product inhibits the shuttle that transports long-chain acyl-CoAs from the cytosol to the mitochondria for oxidation Lipolysis [46] lpl Hydrolyze triglycerides in chylomicrons and very low-density lipoproteins for oxidation or storage Fatty acid transport [14] atgl Catalyze the initial step in triglyceride hydrolysis Lipolysis [49,50] At the end of acclimatization, fish were starved for 24 h before sampling. After being anesthetized with 3-aminobenzoic acid ethyl ester methanesulfonate (MS-222, 10 mg/L), nine fish (three fish for cDNA cloning and six fish for tissue mRNA levels analysis) were randomly selected and dissected on ice.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta

Chen

Luo

Huang

et al. 2014

Euro J Lipid Sci & Tech

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The regulation of lipid metabolism is complex, and is currently an extensive area of research. In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned from the liver of Synechogobius hasta, including g6pd, 6pgd, me, icdh, fas, accα, accβ, lpl, atgl, hsla, hslb, cpt 1a, srebp‐1, pparα, and pparγ. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of S. hasta. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at varying levels. Practical applications: Excessive fat accumulation and disordered lipid metabolism have become serious problems in the sustainable and healthy development of aquaculture. The present study facilitates studies on the regulation of lipid metabolism at the molecular level in fish. The tissue expression profiles of genes increase our understanding of their physiological roles. In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned and characterized from Synechogobius hasta, including g6pd, 6pgd, me, icdh, fas, accα, accβ, lpl, atgl, hsla, hslb, cpt 1a, srebp‐1, pparα, and pparγ. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of S. hasta. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at the varying levels.

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“…Then, cDNA was synthesized using about 3 µg of total RNA with the RevertAid™ H Minus First Strand cDNA Synthesis Kit (Fermentas, Burlington, ON, Canada). The beta-actin gene was used as the reference gene because of its validated stable expression in different tissues and conditions of crucian carp [68,69]. Since β-actin has been widely applied in gene expression as an internal control gene in crucian carp [70,71], we consider that the expression of β-actin is stable and could be used as an efficient and single reference gene in our study [72,73].…”

Section: Methodsmentioning

confidence: 99%

The Transcriptomes of the Crucian Carp Complex (Carassius auratus) Provide Insights into the Distinction between Unisexual Triploids and Sexual Diploids

Kuang

et al. 2014

IJMS

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Both sexual reproduction and unisexual reproduction are adaptive strategies for species survival and evolution. Unisexual animals have originated largely by hybridization, which tends to elevate their heterozygosity. However, the extent of genetic diversity resulting from hybridization and the genomic differences that determine the type of reproduction are poorly understood. In Carassius auratus, sexual diploids and unisexual triploids coexist. These two forms are similar morphologically but differ markedly in their modes of reproduction. Investigation of their genomic differences will be useful to study genome diversity and the development of reproductive mode. We generated transcriptomes for the unisexual and sexual populations. Genes were identified using homology searches and an ab initio method. Estimation of the synonymous substitution rate in the orthologous pairs indicated that the hybridization of gibel carp occurred 2.2 million years ago. Microsatellite genotyping in each individual from the gibel carp population indicated that most gibel carp genes were not tri-allelic. Molecular function and pathway comparisons suggested few gene expansions between them, except for the progesterone-mediated oocyte maturation pathway, which is enriched in gibel carp. Differential expression analysis identified highly expressed genes in gibel carp. The transcriptomes provide information on genetic diversity and genomic differences, which should assist future studies in functional genomics.

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Molecular cloning and tissue distribution of lipoprotein lipase full-length cDNA from Pengze crucian carp (Carassius auratus var. Pengze)

Cited by 26 publications

References 22 publications

Changes in activities and mRNA expression of lipoprotein lipase and fatty acid synthetase in large yellow croaker,Larimichthys crocea(Richardson), during fasting

Changes in activities and mRNA expression of lipoprotein lipase and fatty acid synthetase in large yellow croaker,Larimichthys crocea(Richardson), during fasting

Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta

The Transcriptomes of the Crucian Carp Complex (Carassius auratus) Provide Insights into the Distinction between Unisexual Triploids and Sexual Diploids

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