Molecular cloning and sequencing of 18S rDNA gene fragments from six different ant species

Baur, Axel; Buschinger, Alfred; Zimmermann, Friedrich K.

doi:10.1007/bf01242369

Cited by 20 publications

(16 citation statements)

References 17 publications

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“…The standard nucleic acid techniques of Sambrook et al (1989) were followed. The primers for PCR were designed according to the 18S rDNA sequences of the ant Harpagoxenus sublaevis established by Baur et al (1993) and by comparison of the 5.8S rDNA sequences from man (Nazar et al, 1976; GenBank Accession J01866), yeast Saccharomyces cerevisiae (Rubin, 1973; GenBank Accession K01048), Drosophila melanogaster (Pavlakis et al, 1979; GenBank Accession K01289) and Bombyx rnori (Fujiwara et al, 1982; GenBank Accession X01527).…”

Section: Nucleic Acid Techniquesmentioning

confidence: 99%

“…High molecular DNA was prepard from pupae and adults as previously described (Baur et al, 1993). The standard nucleic acid techniques of Sambrook et al (1989) were followed.…”

Section: Nucleic Acid Techniquesmentioning

confidence: 99%

“…In a series of publications we describe the application of molecular genetic techniques to the solution of these problems. Baur et al (1993) reported the sequences of the 18S rRNA genes of a number of formicine and myrmicine ants which showed high degrees of identity but differed considerably from those of other insect orders.…”

Section: Introductionmentioning

confidence: 97%

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Sequence comparisons of the internal transcribed spacer region of ribosomal genes support close relationships between parasitic ants and their respective host species (Hymenoptera: Formicidae)

et al. 1996

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SummaryA fragment of the internal transcribed spacer (ITS-l) adjacent to the 5.8S rRNA gene of 20 myrmicine ant species was sequenced. Sequence comparisons were carried out between 11 species of the tribe Leptothoracini, five species of the tribe Tetramoriini, three species of the tribe Solenopsidini and one species of the tribe Myrmicini. Additionally, the formicine ant Camponotus ligniperda (tribe Camponotini) was analyzed as an outgroup species. Among all investigated species, the fragment had a variable length of =230-380 bp with only a few conserved sequence elements. The sequences of this fragment were perfectly identical within four palearctic populations of Leptothorax acervorum indicating that intraspecifie variation is rather low. Within the species of Tetramoriini (including Anergates atratulus) 94 A. atratulus has as yet been uncertain, however, sequence comparison of the ITS-1 fragment leads to the conclusion that A. atratulus rather belongs to the tribe Tetramoriini than to the Solenopsidini.

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Section: Nucleic Acid Techniquesmentioning

confidence: 99%

“…High molecular DNA was prepard from pupae and adults as previously described (Baur et al, 1993). The standard nucleic acid techniques of Sambrook et al (1989) were followed.…”

Section: Nucleic Acid Techniquesmentioning

confidence: 99%

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Sequence comparisons of the internal transcribed spacer region of ribosomal genes support close relationships between parasitic ants and their respective host species (Hymenoptera: Formicidae)

et al. 1996

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“…These results form an excellent framework for the study of dolichoderine behavior and systematics, but require testing and extension, especially given that several nodes had very low confidence. Molecular phylogenetic studies of ants are relatively rare so far (Ayala et al, 1996;Baur et al, 1993Baur et al, , 1995Baur et al, , 1996Crozier et al, 1995;Wetterer et al, 1998), although ant sequences have been included in studies of families and orders and, in combination with fossil dating, have yielded an estimate of the time of origin of the group as falling into the Jurassic . The only molecular phylogenetic study of dolichoderine ants was restricted to species of Azteca (Ayala et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

A Molecular Framework for the Phylogeny of the Ant Subfamily Dolichoderinae

Chiotis

Jermiin

Crozier

2000

Molecular Phylogenetics and Evolution

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“…A-and B-chain sequences of rVAA were obtained by PCR from high-molecular, genomic DNA isolated from mistletoe plants according to the method described by Baur et al [14]. The first amplification of a VAA coding sequence was accomplished using oligonucleotides derived from the known amino acid sequence of the N-terminus of the VAA-I A-chain [10,15] and an oligonucleotide derived by homology analysis of known RIP I and A-chains of RIP II molecules.…”

Section: Recombinant Lectinmentioning

confidence: 99%

Effect of a Recombinant Lectin, <i>Viscum album</i> Agglutinin on the Secretion of Interleukin-12 in Cultured Human Peripheral Blood Mononuclear Cells and on NK-Cell-Mediated Cytotoxicity of Rat Splenocytes in vitro and in vivo

Hajtò

Hostanska

Weber³

et al. 1998

Nat Immun

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A plant lectin, Viscum album agglutinin-I (VAA-I) has been shown to increase the number and cytotoxic activity of natural killer (NK) cells in animal models, but the mechanisms underlying these effects are poorly understood. We investigated the effects of the recombinant form of this lectin (rVAA) on secretion of interleukin (IL)-12 and on NK-mediated cytotoxicity against K562 target cells in cultures of human peripheral blood mononuclear cells (PBMC) as well as against YAC-1 target cells in cultured rat spleen cells. In 24-hour cultures of PBMC, 10 ng/ml plant VAA-I and 50 ng/ml rVAA induced significant increases in the secretion of total IL-12. Its biologically active heterodimeric form, p70, was also significantly induced by rVAA. Preincubation of PBMC or splenocytes for 48 h with rVAA in concentrations ranging between 10 pg/ml and 100 pg/ml resulted in moderate enhancements of NK-mediated cytotoxicity. However, coincubation of a low dose of rVAA (100 pg/ml) together with IL-2 and IL-12 (60 U/ml and 2 U/ml, respectively) led to additive stimulation of NK activity. In in vivo experiments, rVAA showed an enhancing effect on NK activity with a bell-shaped curve of efficacy. Forty-eight hours after a single intravenous injection of its most effective doses, 0.5 and 1 ng/kg, into Wistar rats, the NK cytotoxicity of splenocytes against YAC-1 targets doubled, and the frequency of large granular lymphocytes in peripheral blood showed 2.1- and 3-fold increases as compared to control animals. Twenty-four hours following these low lectin doses, the number of large granular lymphocytes was also significantly elevated. After 48 h, 0.5 ng/kg rVAA induced a significant augmentation in the percentage of peripheral Mac-1+ mononuclear cells, including activated monocytes and NK cells. The present results suggest that rVAA augments the secretion of an active form of IL-12 and potentiates the cytokine-induced NK activation. These effects of rVAA may be related to its stimulatory effects on MHC-unrestricted cytotoxicity in vivo.

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Molecular cloning and sequencing of 18S rDNA gene fragments from six different ant species

Cited by 20 publications

References 17 publications

Sequence comparisons of the internal transcribed spacer region of ribosomal genes support close relationships between parasitic ants and their respective host species (Hymenoptera: Formicidae)

Sequence comparisons of the internal transcribed spacer region of ribosomal genes support close relationships between parasitic ants and their respective host species (Hymenoptera: Formicidae)

A Molecular Framework for the Phylogeny of the Ant Subfamily Dolichoderinae

Effect of a Recombinant Lectin, <i>Viscum album</i> Agglutinin on the Secretion of Interleukin-12 in Cultured Human Peripheral Blood Mononuclear Cells and on NK-Cell-Mediated Cytotoxicity of Rat Splenocytes in vitro and in vivo

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