Molecular cloning and quantitative expression of sexually dimorphic markers Dmrt1 and Foxl2 during female-to-male sex change in Epinephelus merra

Alam, M. A.; Kobayashi, Yasuhisa; Horiguchi, Ryo; Hirai, Toshiaki; Nakamura, Masaru

doi:10.1016/j.ygcen.2008.03.018

Cited by 100 publications

(55 citation statements)

References 46 publications

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“…On the contrary, dimorphic expression pattern of male and female sexspecific genes were observed in completely developed testis and ovary. Such dimorphic expression pattern was also reported during gonadal sex differentiation and/ or hormone-induced sex reversal (Alam et al 2008, Ijiri et al 2008. By contrast, in rainbow trout oestrogen treatment up-regulates female specific genes but does not suppress all male specific genes during male-to-female gonadal trans-differentiation (Vizziano-Cantonnet et al 2008).…”

Section: Discussionsupporting

confidence: 56%

Identification of multiple dmrt1s in catfish: localization, dimorphic expression pattern, changes during testicular cycle and after methyltestosterone treatment

Kavarthapu

Senthilkumaran²

2009

Journal of Molecular Endocrinology

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The double sex and mab-3 related (DM) transcription factor 1 (dmrt1) plays an important role in testicular differentiation. Here, we report cloning of multiple dmrt1s, a full-length and two alternative spliced forms from adult catfish (Clarias gariepinus) testis, which encode predicted proteins of 287 (dmrt1a), 253 (dmrt1b) and 233 (dmrt1c) amino acid residues respectively. Interestingly, dmrt1c lacks the majority of the DM domain. Multiple dmrt1s (dmrt1a and dmrt1c) were obtained from Clarias batrachus as well. Tissue distribution (transcript and protein) of catfish dmrt1 revealed exclusive expression in testis. Semi-quantitative RT-PCR revealed the presence of multiple dmrt1s with high levels of dmrt1a in adult testis but not in ovary. Real-time RT-PCR analysis during testicular cycle showed higher levels of dmrt1 transcripts in preparatory and pre-spawning when compared with spawning and post-spawning phases. Immunocytochemical and immunofluorescence localization revealed the presence of catfish Dmrt1 protein in spermatogonia and spermatocytes, which indicates plausible role in spermatogenesis. Histological analysis indicated initiation of gonadal sex differentiation in catfish around 40-50 days after hatching. The potential role for dmrt1 in testicular differentiation is evident from its stage-dependent elevated expression in developing testis. Furthermore, dimorphic expressions of dmrt1s were evident at different stages of gonadal development or recrudescence in catfish. Treatment of methyl testosterone (MT) during early stages of gonadal sex differentiation resulted in adult males. Interestingly, we also obtained MT-treated fishes having ova-testis gonads. Analysis of dmrt1, sox9a, foxl2 and cyp19a1 expression patterns in MT-treated gonads revealed tissue-specific pattern. These results together suggest that multiple dmrt1s are testis-specific markers in catfish.

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Section: Discussionsupporting

confidence: 56%

Identification of multiple dmrt1s in catfish: localization, dimorphic expression pattern, changes during testicular cycle and after methyltestosterone treatment

Kavarthapu

Senthilkumaran²

2009

Journal of Molecular Endocrinology

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“…Moreover, its expression in the ovary is higher than in the testis indicates the sexual dimorphism. The sexual dimorphism of foxl2 indicates the role of Foxl2 in sex differentiation in G. rarus as other animals (Baron et al, 2004;Uda et al, 2004;Wang et al, 2004;Ottolenghi et al, 2005Ottolenghi et al, , 2007Alam et al, 2008). G. rarus foxl2 is different with that of medaka and mouse, in which foxl2 is only expressed in the ovary (Pisarska et al, 2004;Nakamoto et al, 2006).…”

Section: Discussionmentioning

confidence: 98%

Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus

Jiang

Yang

Zhao

et al. 2011

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…In contrast, suppression of DMRT1 transcripts in the gonads was observed in estrogen (E2)-treated male embryos of zebrafish (Schulz et al 2007) and tilapia ). In addition, changes were noticed in DMRT1 expression in concordance with the phases of testicular differentiation in several fishes that change their sexes naturally (Alam et al 2008;He et al 2003;Liarte et al 2007). These findings suggest that DMRT1 is involved not only in early testicular differentiation, but also in gonadal sex reversal/change in teleosts.…”

Section: Introductionmentioning

confidence: 91%

Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse

et al. 2014

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Doublesex-mab 3-related transcription factor 1 (DMRT1) has been identified as the first conserved gene involved in the testicular differentiation of vertebrates. However, the precise role of DMRT1 in spermatogenesis has not been made clear. In this study, immunohistochemical method was used to observe DMRT1 protein localization in order to resolve cellular profile of DMRT1 in the adult testis of three-spot wrasse. DMRT1 protein was clearly and specifically localized in the Sertoli cells of all spermatogenic cells and epithelial cells comprising the efferent duct, but not in the germ cells. In addition, adult males were treated with aromatase inhibitor (AI) for investigating the role of estrogen on the transcription of DMRT1. AI treatment caused an increase in the levels of DMRT1 transcripts in the efferent duct region, concomitant with a decrease in spermatogonia and spermatocytes.Keywords DMRT1 Á Spermatogenesis Á Efferent duct Á Wrasse Á Aromatase inhibitor Á Sertoli cell Abbreviations DMRT1 Doublesex-mab 3-related transcription factor 1 E2

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Molecular cloning and quantitative expression of sexually dimorphic markers Dmrt1 and Foxl2 during female-to-male sex change in Epinephelus merra

Cited by 100 publications

References 46 publications

Identification of multiple dmrt1s in catfish: localization, dimorphic expression pattern, changes during testicular cycle and after methyltestosterone treatment

Identification of multiple dmrt1s in catfish: localization, dimorphic expression pattern, changes during testicular cycle and after methyltestosterone treatment

Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus

Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse

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