Molecular cloning and pharmacological characterization of a Bombyx mori tyramine receptor selectively coupled to intracellular calcium mobilization

Huang, Jia; Ohta, Hiroto; Inoue, Noriko; Takao, Haruka; Kita, Tomo; Ozoe, Fumiyo; Ozoe, Yoshihisa

doi:10.1016/j.ibmb.2009.10.001

Cited by 49 publications

(54 citation statements)

References 36 publications

(47 reference statements)

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“…117 The TYR2-R class is specifically activated by tyramine, but not by other biogenic amines. 150,151 The TYR2-R class is selectively coupled with activation of phospholipase C via the G q family of G proteins and induces intracellular Ca 2+ mobilization (Figure 7), but shows no effect on intracellular cAMP concentration. 150,151 Expression of the TYR2-R class predominantly in the nervous tissue of insects suggests that tyramine may act as a neurotransmitter and neuromodulator, and that these effects may be mediated by binding to the TYR2 class of receptors.…”

Section: Structural and Function Of Octopaminergic And Tyraminergic Rmentioning

confidence: 99%

“…150 Huang et al cloned a cDNA from the nerve tissue of the Bombyx mori silk worm and expressed it in HEK-293 cells. 151 This gene encodes a receptor protein (BmTAR2), which has considerably higher affinity for tyramine than other biogenic amines. BmTAR2 shows a tyramine-induced dose-dependent increase in intracellular Ca 2+ levels (EC 50 11.6 nM), whereas OCT and dopamine increase intracellular Ca 2+ levels only at high concentrations (.100 mM).…”

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confidence: 99%

“…The selective coupling to intracellular Ca 2+ mobilization but no effect on intracellular cAMP concentration suggests that BmTAR2 may also belong to a new family of tyraminergic receptors. 151 These findings favor a revision in the new receptor classification of Evans and Maqueira ( Figure 4B), 149 by adding another subclass in the tyraminergic class of receptors as shown in Figure 4C. 150,151 Furthermore, the author has created a phylogenetic tree based on the comparison of 25 complete nucleotide sequences of insect octopaminergic and tyraminergic receptor genes by using the "Muscle" sequence alignment program.…”

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confidence: 99%

“…151 These findings favor a revision in the new receptor classification of Evans and Maqueira ( Figure 4B), 149 by adding another subclass in the tyraminergic class of receptors as shown in Figure 4C. 150,151 Furthermore, the author has created a phylogenetic tree based on the comparison of 25 complete nucleotide sequences of insect octopaminergic and tyraminergic receptor genes by using the "Muscle" sequence alignment program. [153][154][155] Based on nucleotide sequence homology in the phylogenetic tree, insect octopaminergic receptor sequences from the moth M. sexta (MsOA), 146 D. melanogaster (OAMB) 137 and splice variants (DmOA1A, and DmOA1B), 143 A. mellifera (AmOA1), 138 P. americana (PaOA1), 142 B. mori (BmOAR1), 145 and locust Schistocerca gregaria (SgOctαR) 26 cluster together in the Octα-R class, whereas S. gregaria (SgOctβR), 26 B. mori (BmOAR2), 152 and D. melanogaster (DmOctβ1-R, DmOctβ2-R, DmOctβ3-R) 144 fall into the Octβ-R class ( Figure 5).…”

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confidence: 99%

“…Tyraminergic receptor sequences from A. mellifera (Amtyr1), 148 B. mori (BmTAR1), 140 (HvTA), 134 Agrotis ipsilon (Aityr/OCT), 141 Locusta migratoria (Lmtyr1), 139 and the cattle tick Boophilus microplus (BmiTA) 147 fall into the Tyr1-R subclass. However, B. mori (BmTAR2), 151 D. melanogaster (DmTA1 or Tyr-Dro), 132 D. melanogaster (DmTA2, DmCG7431, DmCG16766), 150 A. mellifera (Amtyr-ike), 150 and A. gambiae genome sequences (GPRNNA4) 150 cluster together in the TYR2-R subclass ( Figure 5). This phylogenetic tree further supports applicability of the new Evans and Maqueira classification scheme, except that there are two subclasses in the tyraminergic receptor class.…”

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confidence: 99%

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Review of octopamine in insect nervous systems

Farooqui¹

2012

OAIP

145

155

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Octopamine (OCT) belongs to a group of compounds known as biogenic amines. OCT, a monohydroxylic analog of norepinephrine, is found in both vertebrate and invertebrate nervous systems. OCT is present in relatively high concentrations in the neuronal and nonneuronal tissues of most invertebrate species studied. However, OCT occurs as a trace amine in vertebrates where its physiological significance remains uncertain. OCT acts as a neurotransmitter, neuromodulator, and neurohormone in insect nervous systems where it prominently influences multiple physiological events. In the peripheral nervous system, OCT modulates the activity of flight muscles, peripheral organs, and most sense organs. In the central nervous system, OCT is essential for the regulation of motivation, desensitization of sensory inputs, arousal, initiation, and maintenance of various rhythmic behaviors, hygiene behavior, and complex social behaviors, including establishment of labor, as well as learning and memory. As a neurotransmitter, OCT regulates endocrine gland activity and controls the emission of light in the firefly lantern. As a neurohormone, OCT is released into hemolymph, transported to target tissues, and induces mobilization of lipids and carbohydrates, preparing insects for a period of extended activity or assisting recovery from a period of increased energy demand. OCT modulates hemocytic nodulation in nonimmune larvae and enhances phagocytosis as a neurohormone. OCT exerts its effects by binding to specific receptors belonging to the superfamily of G protein-coupled receptors and shares the structural motif of seven transmembrane domains. Activation of octopaminergic receptor types is coupled with different second messenger pathways depending on the species, tissue source, receptor type, and cell line used for expression of the cloned receptor. OCT-mediated generation of second messengers is associated with changes in cellular response, affecting insect behaviors. This review describes the roles of OCT in insect nervous systems at the behavioral and molecular levels.

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Section: Structural and Function Of Octopaminergic And Tyraminergic Rmentioning

confidence: 99%

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confidence: 99%

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Review of octopamine in insect nervous systems

Farooqui¹

2012

OAIP

145

155

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Characterization and functional analysis of a β‐adrenergic‐like octopamine receptor from the oriental armyworm (Mythimna separata Walker)

Guo

Qiu

Chen

et al. 2021

Arch Insect Biochem Physiol

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The β‐adrenergic‐like octopamine receptor (OA2B2), which binds the biogenic amine octopamine, belongs to the class of G‐protein coupled receptors and significantly regulates many physiological and behavioral processes in insects. In this study, the putative open reading frame sequence of the MsOA2B2 gene in Mythimna separata was cloned, the full‐length complementary DNA was 1191 bp and it encoded a 396‐amino acid protein (GenBank accession number MN822800). Orthologous sequence alignment, phylogenetic tree analysis, and protein sequence analysis all showed that the cloned receptor belongs to the OA2B2 protein family. Real‐time quantitative polymerase chain reaction of spatial and temporal expression analysis revealed that the MsOAB2 gene was expressed in all developmental stages of M. separata and was most abundant in egg stages and second and fourth instars compared with other developmental stages, while the expression level during the pupal stage was much lower than that at the other stages. Further analysis with sixth instar M. separata larvae showed that the MsOA2B2 gene was expressed 1.81 times higher in the head than in integument and gut tissues. Dietary ingestion of dsMsOA2B2 significantly reduced the messenger RNA level of MsOA2B2 and decreased mortality following amitraz treatment. This study provides both a pharmacological characterization and the gene expression patterns of OA2B2 in M. separata, facilitating further research for insecticides using MsOA2B2 as a target.

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Neuronal distribution of tyramine and the tyramine receptor AmTAR1 in the honeybee brain

Thamm

Scholl

Reim

et al. 2017

J of Comparative Neurology

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Tyramine is an important neurotransmitter, neuromodulator, and neurohormone in insects. In honeybees, it is assumed to have functions in modulating sensory responsiveness and controlling motor behavior. Tyramine can bind to two characterized receptors in honeybees, both of which are coupled to intracellular cAMP pathways. How tyramine acts on neuronal, cellular and circuit levels is unclear. We investigated the spatial brain expression of the tyramine receptor AmTAR1 using a specific antibody. This antibody detects a membrane protein of the expected molecular weight in western blot analysis. In honeybee brains, it labels different structures which process sensory information. Labeling along the antennal nerve, in projections of the dorsal lobe and in the gnathal ganglion suggest that tyramine receptors are involved in modulating gustatory and tactile perception. Furthermore, the ellipsoid body of the central complex and giant synapses in the lateral complex show AmTAR1-like immunoreactivity (AmTAR1-IR), suggesting a role of this receptor in modulating sky-compass information and/or higher sensor-motor control. Additionally, intense signals derive from the mushroom bodies, higher-order integration centers for olfactory, visual, gustatory and tactile information. To investigate whether AmTAR1-expressing brain structures are in vicinity to tyramine releasing sites, a specific tyramine antibody was applied. Tyramine-like labeling was observed in AmTAR1-IR positive structures, although it was sometimes weak and we did not always find a direct match of ligand and receptor. Moreover, tyramine-like immunoreactivity was also found in brain regions without AmTAR1-IR (optic lobes, antennal lobes), indicating that other tyramine-specific receptors may be expressed there.

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Molecular cloning and pharmacological characterization of a Bombyx mori tyramine receptor selectively coupled to intracellular calcium mobilization

Cited by 49 publications

References 36 publications

Review of octopamine in insect nervous systems

Review of octopamine in insect nervous systems

Characterization and functional analysis of a β‐adrenergic‐like octopamine receptor from the oriental armyworm (Mythimna separata Walker)

Neuronal distribution of tyramine and the tyramine receptor AmTAR1 in the honeybee brain

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