Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic bovine viral diarrhea virus strain SD-1

Deng, Ruitang; Brock, Kenny V.

doi:10.1016/0042-6822(92)90262-n

Cited by 202 publications

(151 citation statements)

References 46 publications

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“…They occur worldwide and are the causative agents of economically important animal diseases such as classical swine fever, bovine viral diarrhoea and border disease of sheep. Like other members of the family, pestiviruses are plus-stranded RNA viruses whose genome comprises one long open reading frame (Collett et al, 1988 ;Meyers et al, 1989 ;Moormann et al, 1990 ;Deng et al, 1992). Translation into one hypothetical polyprotein is accompanied by proteolytic processing through host cell and virus-encoded proteases.…”

Section: Introductionmentioning

confidence: 99%

Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles.

Weiland

Unger

et al. 1999

Journal of General Virology

100

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The glycoproteins E rns of classical swine fever virus (CSFV) and E rns and E2 of bovine viral diarrhoea virus (BVDV) are shown to be located at the surface of infected cells by the use of indirect immunofluorescence and by cytofluorometric analysis. The positive immunostaining of the cell surface was further analysed by immunogold electron microscopy and it could be shown that only extracellular virions were labelled. Gold granules were not seen at the cellular plasma membrane. In contrast to BVDV E2, the CSFV E2 of virions sticking to the plasma membrane was not accessible to the respective monoclonal antibodies. However, CSFV particles isolated from culture supernatant were able to bind both monoclonal anti-E rns and anti-E2 antibodies. For CSFV and BVDV, binding of anti-E rns antibodies to the virions was more pronounced than that of anti-E2. This finding was unexpected since E2 is considered to be the immunodominant glycoprotein.

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Section: Introductionmentioning

confidence: 99%

Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles.

Weiland

Unger

et al. 1999

Journal of General Virology

100

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“…BVDV is an enveloped virus with a positive-stranded RNA genome of about 12.5 kb, which comprises a single open reading frame (ORF) flanked at the 5' and 3' ends by untranslated regions (UTRs) (Collett et al, 1988;Deng and Brock, 1992). The ORF encodes a polyprotein composed of approximately 4000 amino acids that is co-and posttranslationally processed by both viral and cellular proteases into 11 or 12 mature viral proteins: NH2-N pro , C, E rns , E1, E2, p7, NS2-3, NS4A, NS4B, NS5A, NS5B-COOH (Rice et al, 1996;Thiel et al, 1996).…”

mentioning

confidence: 99%

Cytopathogenicity markers in the genome of Hungarian cytopathic isolates of bovine viral diarrhoea virus

Bálint¹,

Baule

Kecskeméti

et al. 2005

Acta Veterinaria Hungarica

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Since genetic recombination is a major factor in the evolution of the cytopathogenic (cp) bovine viral diarrhoea virus (BVDV) biotypes, in this study the cytopathogenicity markers were investigated in the genomes of two cp BVDV strains recently isolated from mucosal disease (MD) cases in Hungary. In the genome of strain H4956, a Jiv-like insertion was found similar to those described in reference strain NADL and in other BVDV 1, BVDV 2 and border disease virus (BDV) strains. The 133 amino acid Jiv-like sequence is inserted at nucleotide position 4984 (amino acid position 1533), 9 nucleotides upstream of that of strain NADL. The insertion showed 96% amino acid sequence identity with the cellular Jiv protein. In the genome of cp BVDV strain H115/PCR, an ubiquitin-containing duplication was found. The duplicated sequence started at nucleotide position 7978 (amino acid 2531) in the NS4B gene. The duplication contained a complete ubiquitin monomer of 76 amino acids and the complete NS3 gene starting at nucleotide position 5153 (amino acid 1589), which corresponds to the first Nterminal amino acid of NS3. The duplication was located further downstream of the known ubiquitin-containing genomic regions of cp BVDV strains, and it consisted of the shortest inserted nucleotide sequence. The insertions and duplication of strains H4956 and H115/PCR further confirmed that recombinations occurring at positions A and B are the most common mechanisms leading to the development of BVDV cytopathogenicity.

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“…BVDV is an enveloped virus with an uncapped and non-polyadenylated positive-stranded RNA genome of about 12.5 kb in size. The genome comprises a single open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) [11,12,14]. The 5'UTR function is a so-called internal ribosome entry site (IRES) that promotes cap-independent translation initiation [30,31].…”

Section: Introductionmentioning

confidence: 99%

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

Bálint¹,

Baule

Pálfi³

et al. 2005

Vet. Res.

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-A live bovine viral diarrhea (BVDV) vaccine, marketed as a derivate of the Oregon C24V strain, was used between the end of the 1960s and the beginning of the 1990s in Central Europe. Since laboratory investigations of mucosal disease cases in vaccinated animals suggested recombinations between the vaccine and wild type variants of BVDV, and recombinational nucleotide sequences seemed distinct from BVDV Oregon C24V, the aim of the present retrospective study was to analyze the genomes of pre-registration (termed here BVDV-Xpre) and of marketed (BVDV-X) batches of the vaccine. The results of the complete genome analysis of BVDV-Xpre confirmed that the original virus strain used at the start of the vaccine production was Oregon C24V. Surprisingly, the analysis of the complete nucleotide sequence of the BVDV-X marketed vaccine revealed that this strain belongs to the BVDV 1b subgroup, with a 93.7% nucleotide sequence homology to BVDV reference strain Osloss. The homology to BVDV Oregon C24V was significantly lower (77.4%), and a thorough sequence scanning showed that the genome of BVDV-X had not derived from Oregon C24V. These data indicate the very likely scenario that a strain different to Oregon C24V was picked up during the in vitro or in vivo passages for vaccine development. Despite of the virus-switch, the BVDV-X vaccine continuously maintained its innocuity and efficacy, as proven by the regular quality testing data, and the presence of the foreign virus remained unnoticed over many years. The results of this work emphasize that the contamination of commercially available live vaccines with exogenous BVDV strains is a real risk factor, and a unequivocal analysis, including molecular methods, is needed to verify their authenticity. bovine viral diarrhea virus / vaccine / strain switch / control

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Molecular cloning and nucleotide sequence of a pestivirus genome, noncytopathic bovine viral diarrhea virus strain SD-1

Cited by 202 publications

References 46 publications

Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles.

Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles.

Cytopathogenicity markers in the genome of Hungarian cytopathic isolates of bovine viral diarrhoea virus

Retrospective genome analysis of a live vaccine strain of bovine viral diarrhea virus

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