Molecular Cloning and Induction of Bovine Prostaglandin E Synthase by Gonadotropins in Ovarian Follicles Prior to Ovulationin Vivo

Bouchard, Nadine; Goff, A.K.; Lussier, Jacques G.; Sirois, Jean

doi:10.1074/jbc.m103709200

Cited by 93 publications

(68 citation statements)

References 61 publications

(91 reference statements)

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“…In primates, mural granulosa and theca cells of periovulatory follicles express the three forms of PTGES (Duffy et al 2005), of which only PTGS1 exhibits a gonadotropinstimulated expression. The gonadotropin-dependent induction of PTGS1 has also been observed in bovine and mouse mural granulosa cells (Filion et al 2001, Sun et al 2006. However, PTGES have never been studied in the COC at any stage of differentiation, despite the proven importance of PGE 2 to the developmental competence of oocytes in mammals.…”

Section: Introductionmentioning

confidence: 96%

Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus–oocyte complexes during in vitro maturation and fertilization

Nuttinck¹,

Guienne²,

Clément³

et al. 2008

Reproduction

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Prostaglandin E 2 (PGE 2 ) and progesterone appear to be critical mediators of cumulus expansion and the resumption of oocyte meiosis. The aim of this study was to identify the types of prostaglandin E synthase (PTGES) expressed in the bovine cumulus-oocyte complex (COC), to characterize their temporal expression during the periconceptional interval using an in vitro model of maturation (IVM) and fertilization (IVF), and to compare their expression with the level of steroidogenic gene expression. Real-time RT-PCR analysis revealed that enzymes related to the PGE 2 biosynthesis pathway were mainly expressed during IVM. Transcripts encoding PTGES1-3 were detected in bovine COCs. Only the expression of PTGES1 significantly increased during IVM whereas that of PTGES2 and PTGES3 remained unchanged. The induction of PTGES1 expression paralleled the induction of prostaglandin G/H synthase-2 (PTGS2) expression and the amounts of PGE 2 secreted by maturing COCs. Concomitantly, cholesterol side chain cleavage cytochrome P450 expression was significantly upregulated in maturing COCs and the high level of expression persisted in fertilized COCs. The expression of the StAR protein remained constant during IVM and then decreased significantly during IVF. Expression of the progesterone catabolic-related enzyme, 20a-hydroxysteroid dehydrogenase significantly decreased throughout the periconceptional interval. This was associated with a rising level of progesterone released by COCs in the culture media. In conclusion, our results suggest that the periconceptional differentiation of the bovine COC includes the transient induction of PGE 2 biosynthetic activity via the PTGS2/PTGES1 pathway during the maturation period and the increasing ability to produce progesterone from the immature to the fertilized stages. Reproduction (2008) 135 593-603

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Section: Introductionmentioning

confidence: 96%

Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus–oocyte complexes during in vitro maturation and fertilization

Nuttinck¹,

Guienne²,

Clément³

et al. 2008

Reproduction

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“…These observations imply a crucial role for COX-2-derived PGE 2 in the female reproductive process. Even though induction of mPGES-1 expression has been observed during the period of pregnancy (21), no abnormality has so far been found in the reproductive system of mPGES-1-deficient mice (23). Moreover, although mice deficient in both COX-1 and COX-2 (41) or the PGE receptor EP4 (42) frequently undergo neonatal death due to failure of closure of the ductus arteriosus, mPGES-1-null mice are all viable after birth (23), indicative of no gross abnormality in this developmental process.…”

Section: Mpges-2 and Pge 2 Productionmentioning

confidence: 99%

“…Among them, a perinuclear membrane-bound form of PGES belonging to the MAPEG (for membrane-associated proteins involved in eicosanoid and glutathione metabolism) family, which we herein call mPGES-1, has been well characterized by several recent studies (14,(17)(18)(19)(20)(21)(22)(23)(24)(25). The expression of mPGES-1 is up-regulated by proinflammatory stimuli and down-regulated by anti-inflammatory glucocorticoids, often in accordance with that of COX-2 (14,(17)(18)(19)(20)(21)(22)(23)(24)(25). mPGES-1 displays functional coupling with COX-2 in marked preference to COX-1, even though COX-1/mPGES-1 coupling can occur if a large amount of AA is supplied (14).…”

mentioning

confidence: 99%

Cellular Prostaglandin E2 Production by Membrane-bound Prostaglandin E Synthase-2 via Both Cyclooxygenases-1 and -2

Murakami

Nakashima

Kamei

et al. 2003

Journal of Biological Chemistry

313

308

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Current evidence suggests that two forms of prostaglandin (PG) E synthase (PGES), cytosolic PGES and membrane-bound PGES (mPGES) -1, preferentially lie downstream of cyclooxygenase (COX) -1 and -2, respectively, in the PGE 2 biosynthetic pathway. In this study, we examined the expression and functional aspects of the third PGES enzyme, mPGES-2, in mammalian cells and tissues. mPGES-2 was synthesized as a Golgi membrane-associated protein, and spontaneous cleavage of the N-terminal hydrophobic domain led to the formation of a truncated mature protein that was distributed in the cytosol with a trend to be enriched in the perinuclear region. In several cell lines, mPGES-2 promoted PGE 2 production via both COX-1 and COX-2 in the immediate and delayed responses with modest COX-2 preference. In contrast to the marked inducibility of mPGES-1, mPGES-2 was constitutively expressed in various cells and tissues and was not increased appreciably during tissue inflammation or damage. Interestingly, a considerable elevation of mPGES-2 expression was observed in human colorectal cancer. Collectively, mPGES-2 is a unique PGES that can be coupled with both COXs and may play a role in the production of the PGE 2 involved in both tissue homeostasis and disease.Biosynthesis of prostaglandin (PG) 1 E 2 , which is produced by a variety of cells and tissues and exhibits diverse bioactivities, is mediated by three enzymatic reactions involving phospholipase A 2 (PLA 2 ), cyclooxygenase (COX), and PGE synthase (PGES). In this biosynthetic pathway, arachidonic acid (AA) released from membrane phospholipids by cytosolic or secretory PLA 2 s is converted to PGH 2 by COX-1 or COX-2 and is then isomerized to PGE 2 by terminal PGES enzymes.The constitutive COX-1 mainly promotes immediate PG production elicited by agonists promptly mobilizing intracellular Ca 2ϩ , a situation in which a burst release of AA occurs (1-5).The inducible COX-2 is essential for delayed PG generation induced by proinflammatory stimuli, during which AA is gradually supplied over long periods, and also promotes immediate PG production if it already exists in cells primed by particular stimuli (1-5). Current studies employing isozyme-specific inhibitors and knockout mice have revealed that the two COXs play distinct roles in vivo (6 -10), and segregated utilization of these enzymes at the cellular level has been explained not only by their distinct expression profiles but also by subtle differences in their AA requirement, hydroperoxide sensitivity, and subcellular localization (4, 11-13). In addition, selective coupling with various terminal PG synthases has also been shown to influence crucially the utilization of the two COX isoforms during the different phases of cell activation (14 -16). PGES enzymes, which lie downstream of COXs, occur in multiple forms in mammalian cells (1). Among them, a perinuclear membrane-bound form of PGES belonging to the MAPEG (for membrane-associated proteins involved in eicosanoid and glutathione metabolism) family, which we herein cal...

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“…Only two of the identified genes, PTGES and PSAP, have previously been associated with ovarian function, specifically luteinisation of granulosa cells (Filion et al 2001, McRae et al 2005.…”

Section: Sage Analysis Of Differentially Expressed Transcripts In Thementioning

confidence: 99%

Differentiation of the bovine dominant follicle from the cohort upregulates mRNA expression for new tissue development genes

Mihm¹,

Baker²,

Fleming³

et al. 2008

Reproduction

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This study was designed to identify genes that regulate the transition from FSH-to LH-dependent development in the bovine dominant follicle (DF). Serial analysis of gene expression (SAGE) was used to compare the transcriptome of granulosa cells isolated from the most oestrogenic growing cohort follicle (COH), the newly selected DF and its largest subordinate follicle (SF) which is destined for atresia. Follicle diameter, follicular fluid oestradiol (E) and E:progesterone ratio confirmed follicle identity. Results show that there are 93 transcript species differentially expressed in DF granulosa cells, but only 8 of these encode proteins known to be involved in DF development. Most characterised transcripts upregulated in the DF are from tissue development genes that regulate cell differentiation, proliferation, apoptosis, signalling and tissue remodelling. Semiquantitative real-time PCR analysis confirmed seven genes with upregulated (P%0.05) mRNA expression in DF compared with both COH and SF granulosa cells. Thus, the new genes identified by SAGE and real-time PCR, which show enhanced mRNA expression in the DF, may regulate proliferation (cyclin D2; CCND2), prevention of apoptosis or DNA damage (growth arrest and DNA damage-inducible, b; GADD45B), RNA synthesis (splicing factor, arginine/serine rich 9; SFRS9) and unknown processes associated with enhanced steroidogenesis (ovary-specific acidic protein; DQ004742) in granulosa cells of DF at the onset of LH-dependent development. Further studies are required to show whether the expression of identified genes is dysregulated when abnormalities occur during DF selection or subsequent development.

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Molecular Cloning and Induction of Bovine Prostaglandin E Synthase by Gonadotropins in Ovarian Follicles Prior to Ovulationin Vivo

Cited by 93 publications

References 61 publications

Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus–oocyte complexes during in vitro maturation and fertilization

Expression of genes involved in prostaglandin E2 and progesterone production in bovine cumulus–oocyte complexes during in vitro maturation and fertilization

Cellular Prostaglandin E2 Production by Membrane-bound Prostaglandin E Synthase-2 via Both Cyclooxygenases-1 and -2

Differentiation of the bovine dominant follicle from the cohort upregulates mRNA expression for new tissue development genes

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