Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus

Bobanović, Laura K.; Laine, Mika; Petersen, Carl C. H.; Bennett, Deborah L.; Berridge, Michael J.; Lipp, Peter; Ripley, Scott J.; Bootman, Martin D.

doi:10.1042/bj3400593

Cited by 26 publications

(17 citation statements)

References 41 publications

(26 reference statements)

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“…In turn, emptying of Ca 2+ stores has been proposed as a trigger for transient receptor potential cation channel (TRPC) activation leading to Ca 2+ influx (37). Xenopus TRPC1 (xTRPC1) has been cloned (38) and is expressed in embryonic spinal neurons (39,40). Pharmacological inhibition of TRPCs or molecular knockdown of xTRPC1 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord

Belgacem

Borodinsky

2011

Proc. Natl. Acad. Sci. U.S.A.

137

198

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Evolutionarily conserved hedgehog proteins orchestrate the patterning of embryonic tissues, and dysfunctions in their signaling can lead to tumorigenesis. In vertebrates, Sonic hedgehog (Shh) is essential for nervous system development, but the mechanisms underlying its action remain unclear. Early electrical activity is another developmental cue important for proliferation, migration, and differentiation of neurons. Here we demonstrate the interplay between Shh signaling and Ca 2+ dynamics in the developing spinal cord. Ca 2+ imaging of embryonic spinal cells shows that Shh acutely increases Ca 2+ spike activity through activation of the Shh coreceptor Smoothened (Smo) in neurons. Smo recruits a heterotrimeric GTP-binding protein-dependent pathway and engages both intracellular Ca 2+ stores and Ca 2+ influx. The dynamics of this signaling are manifested in synchronous Ca 2+ spikes and inositol triphosphate transients apparent at the neuronal primary cilium. Interaction of Shh and electrical activity modulates neurotransmitter phenotype expression in spinal neurons. These results indicate that electrical activity and second-messenger signaling mediate Shh action in embryonic spinal neurons.

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Section: Resultsmentioning

confidence: 99%

Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord

Belgacem

Borodinsky

2011

Proc. Natl. Acad. Sci. U.S.A.

137

198

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“…We first examined the expression of XTRPC1 in Xenopus spinal neurons with previously characterized antibodies 31 . We found that XTRPC1 was expressed in the majority of Xenopus spinal neurons in the culture (Fig.…”

Section: Expression Of Xtrpc1 At Growth Cones Of Spinal Neuronsmentioning

confidence: 99%

“…For immunocytochemistry, cultured cells derived from embryos unilaterally injected at the twocell stage were processed as previously described 48,49 . Polyclonal antibodies against two different regions of XTRPC1 have been previously characterized 31 and were used at a dilution of 1:100 for immunocytochemistry of cell cultures and at a dilution of 1:1,000 for western blot. Similar results were obtained with both antibodies.…”

Section: Xenopus Embryo Injection Cell Culture Immunocytochemistry mentioning

confidence: 99%

“…Notably, TRPC5 regulates growth cone morphology and neurite extension in cultured hippocampal neurons 29,30 . XTRPC1, a homolog of mammalian TRPC1, is the only TRPC family member cloned so far from Xenopus 31,32 . In this study, with morpholinomediated gene knockdown, expression of dominant-negative channels and pharmacological inhibitors, we examined the role of XTRPC1 in Xenopus spinal neuron growth cone turning responses to gradients of specific guidance cues and inhibitory factors for axon regeneration, including netrin-1, BDNF, myelin-associated glycoprotein (MAG) and semaphorin 3A (Sema3A).…”

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XTRPC1-dependent chemotropic guidance of neuronal growth cones

et al. 2005

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Calcium arising through release from intracellular stores and from influx across the plasma membrane is essential for signalling by specific guidance cues and by factors that inhibit axon regeneration. The mediators of calcium influx in these cases are largely unknown. Transient receptor potential channels (TRPCs) belong to a superfamily of Ca 2+ -permeable, receptoroperated channels that have important roles in sensing and responding to changes in the local environment. Here we report that XTRPC1, a Xenopus homolog of mammalian TRPC1, is required for proper growth cone turning responses of Xenopus spinal neurons to microscopic gradients of netrin-1, brain-derived neurotrophic factor and myelin-associated glycoprotein, but not to semaphorin 3A. Furthermore, XTRPC1 is required for midline guidance of axons of commissural interneurons in the developing Xenopus spinal cord. Thus, members of the TRPC family may serve as a key mediator for the Ca 2+ influx that regulates axon guidance during development and inhibits axon regeneration in adulthood.Axons are guided to their targets in the developing nervous system by diffusible and bound cues that either attract or repel the growing tip of an axon, the growth cone [1][2][3][4] . Once neuronal circuits are formed, the ability of axons to regenerate after injury or disease in the mature mammalian CNS is extremely limited, largely because of the presence of inhibitory factors [5][6][7][8][9][10] . During the last decade, an array of evolutionarily conserved guidance cues and © 2005 Nature Publishing Group Correspondence should be addressed to G.L.M. (gming1@bs.jhmi.edu). Note: Supplementary information is available on the Nature Neuroscience website. COMPETING INTERESTS STATEMENTThe authors declare that they have no competing financial interests. NIH Public Access NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript their receptors have been identified and have been shown to be involved in axon guidance during development and in axon regeneration in the adult central nervous system [1][2][3]6 . How specific guidance signals are transduced from receptor activation to rearrangement of cytoskeleton within the neuronal growth cone is just beginning to be elucidated [1][2][3][4]11,12 . One of the initial signal transduction mechanisms that triggers the growth cone responses to a large number of guidance cues and inhibitory factors associated with myelin is an increase in cytosolic Ca 2+ , arising from both intracellular store release and influx across the plasma membrane 5,7,[13][14][15][16][17] . Notably, different patterns of Ca 2+ elevation trigger differential attractive and repulsive turning responses of the growth cone 13,18 . While previous studies have demonstrated the essential role of Ca 2+ signalling in axon guidance and regeneration both in vitro and in vivo 5,7,[11][12][13][15][16][17][18][19][20][21][22][23][24] , it is unknown how initial Ca 2+ mobilization from intracellular stores is coupled to sustained Ca 2+ influx from the extr...

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“…Akaike and colleagues (37) showed that degenerate hTRP-1 oligonucleotides reduced thapsigargin-stimulated currents in oocytes injected with a rat TRP4 homolog. More recently, Berridge and colleagues have cloned Xtrp1 (38), but its function remains unreported. Our preliminary experiments failed to demonstrate reduction in CD16.7-PKD1(115-226)-associated cation channel activity after coinjection of Xtrp1 antisense oligonucleotides (data not shown).…”

Section: Importance Of Selected Serine and Tyrosine Residues For Catimentioning

confidence: 99%

The Cytoplasmic C-terminal Fragment of Polycystin-1 Regulates a Ca2+-permeable Cation Channel

Vandorpe

Chernova

Jiang

et al. 2001

Journal of Biological Chemistry

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The cytoplasmic C-terminal portion of the polycystin-1 polypeptide (PKD1(1-226)) regulates several important cell signaling pathways, and its deletion suffices to cause autosomal dominant polycystic kidney disease. However, a functional link between PKD1 and the ion transport processes required to drive renal cyst enlargement has remained elusive. We report here that expression at the Xenopus oocyte surface of a transmembrane fusion protein encoding the C-terminal portion of the PKD1 cytoplasmic tail, PKD1 (115-226

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Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus

Cited by 26 publications

References 41 publications

Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord

Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord

XTRPC1-dependent chemotropic guidance of neuronal growth cones

The Cytoplasmic C-terminal Fragment of Polycystin-1 Regulates a Ca2+-permeable Cation Channel

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