Molecular cloning and identification of eukaryotic translation initiation factor 1 family genes (eIF1,eIF1AandeIF1B) inLeymus chinensis(Trin.)

Abstract

Eukaryotic translation initiation factor 1 family genes (eIF1, eIF1A and eIF1B) play important roles in the 43S complex formation during the mRNA translation of protein synthesis. In particular, eIF1A is stabilizing the binding of Met-tRNA to the 40S ribosomal subunit, promoting the mRNA binding and preventing the premature association of the 40S ribosomal subunit to the 60S ribosomal subunit. eIF1 helps the mRNA scanning for the location recognition of the AUG initiation codon. However, little is known for another eIF1 family gene À eIF1B, especially in plants. In this work, we collected a high salt-tolerant grass Leymus chinensis (Trin.) as the object of our study. We cloned and sequenced the eIF1 family genes from this species. Based on the DNA sequence alignment and the analysis of existing sequences in NCBI's GenBank database, effective primer sets, specific for L. chinensis, were newly designed. Using these primer sets, the full-length cDNAs of corresponding eIF1 family genes were obtained, and we had submitted these sequencing results to NCBI's GenBank database. The basic local alignment search tool result showed that our sequences were highly identical to relevant existing eIF1 family genes in NCBI's GenBank database. Combined with existing sequence sources, we also constructed phylogenetic trees to further understand the relationship between eIF1 family genes of L. chinensis and other species. This work would provide more eIF1 sequence sources and will lay the foundations for studying and further understanding of the eIF1 gene functions in stress tolerance mechanisms.

Novel Ser74 of NF-κB/CgIκBα Phosphorylated by MAPK/ERK Regulates Temperature Adaptation in Oysters

“…Ribosomal proteins have emerged as significant markers for physiological status evaluation under stress conditions in marine organisms (Jiao, Dai et al, 2021, Vieira, Braga et al, 2018. Eif1b participates in the assembly of the 43S complex during the mRNA translation of protein synthesis (Sun, Gong et al, 2015), remodeling the protein landscape to enhance stress survival during heat stress (Neef & Thiele, 2009), which is consistent with our previous findings (Wang et al, 2023a). Furthermore, Gst, a well-known antioxidant gene, shows increased expression and enzyme activity in marine organisms under high-temperature stress, aiding in combating oxidative stress induced by elevated temperatures (Guerriero, Bassem et al, 2018, Missionário, Travesso et al, 2023, Teixeira, Diniz et al, 2013.…”

Effects of Alkalinity Exposure on Antioxidant Status, Metabolic Function, and Immune Response in the Hepatopancreas of Macrobrachium nipponense

“…Eukaryotic translation initiation factor 2 (eIF2) is a key protein involved in translation initiation of eukaryotic cells. It plays essential roles in the conversion of eIF2-GDP (inactive state of eIF2) into eIF2-GTP (active state of eIF2) during the process of translation initiation [68,69]. Ribosomes regulate the process of RNA translation into protein and can obtain the genetic information from messenger RNA and convert it into amino acid sequences to synthesize proteins [70,71].…”