2006
DOI: 10.1111/j.1365-2583.2006.00676.x
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Molecular cloning and heterologous expression of an α‐adrenergic‐like octopamine receptor from the silkworm Bombyx mori

Abstract: A cDNA encoding an octopamine (OA) receptor (BmOAR1) was isolated from the nerve tissue of silkworm (Bombyx mori) larvae. Comparison of amino acid sequences showed that BmOAR1 is highly identical to OA receptors isolated from Periplaneta americana (Pa oa(1)), Apis mellifera (AmOA1), and Drosophila melanogaster (OAMB or DmOA1A). BmOAR1 was stably expressed in HEK-293 cells. OA above 1 microM led to an increase in intracellular cyclic AMP concentration ([cAMP](i)). The synthetic OA-receptor agonist demethylchlor… Show more

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Cited by 57 publications
(61 citation statements)
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“…octopamine, inhibits adenylyl cyclase activity via coupling to inhibitory G proteins, inducing a decrease in intracellular cAMP levels ( Figure 7). 134,139,141,145,148 However, in other preparations, octopamine is more or equally as effective as tyramine in increasing intracellular Ca 2+ release. 135,136 These findings suggest that cloned receptors when expressed in different cell lines may be coupled with multiple effector pathways involving different G proteins.…”
Section: Structural and Function Of Octopaminergic And Tyraminergic Rmentioning
confidence: 99%
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“…octopamine, inhibits adenylyl cyclase activity via coupling to inhibitory G proteins, inducing a decrease in intracellular cAMP levels ( Figure 7). 134,139,141,145,148 However, in other preparations, octopamine is more or equally as effective as tyramine in increasing intracellular Ca 2+ release. 135,136 These findings suggest that cloned receptors when expressed in different cell lines may be coupled with multiple effector pathways involving different G proteins.…”
Section: Structural and Function Of Octopaminergic And Tyraminergic Rmentioning
confidence: 99%
“…These receptors exert a higher affinity for OCT than tyramine and are coupled with an increase in intracellular Ca 2+ concentration as well as a small increase in intracellular cAMP levels. 137,138,141,143,145 The OCTβ-R class shows sequence similarities with vertebrate β-adrenergic receptors, and activation of receptors in response to OCT specifically results in increased intracellular cAMP levels. 144,149 The OCTβ-R class is subdivided into several subclasses, which are pharmacologically different from each other.…”
mentioning
confidence: 99%
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“…8) A cDNA fragment was amplified by RT-PCR using primers designed based on the known sequences of Drosophila and Limnaea OA receptors, and 3Ј and 5Ј end sequences were obtained by RACE. The obtained full-length cDNA was found to encode a protein that contains seven transmembrane domains (TMs) and conserved amino acids characteristic of biogenic amine receptors.…”
Section: Octopamine Receptormentioning
confidence: 99%