Molecular cloning and functional expression of the first two specific insect myosuppressin receptors

Egerod, Kristoffer L.; Reynisson, Eyjólfur; Hauser, Frank; Cazzamali, Giuseppe; Grimmelikhuijzen, Cornelis J.P.

doi:10.1073/pnas.1632197100

Cited by 86 publications

(93 citation statements)

References 37 publications

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“…As for BMSR, the EC 50 for BMS in the cAMP assay (0.089 nM) was Ϸ400 times lower than that in the heterologous expression system (32 nM) (12). However, this relatively high value is comparable with the EC 50 of Drosophila myosuppressin (DMS) (40 nM) that is necessary for the activation of two DMS receptors expressed in a similar heterologous system (27). Taking these relative differences into consideration, it appears that the responsiveness of BMSR to BRFa corresponds well to the effective concentrations in the cAMP and ecdysteroid assays (see Fig.…”

Section: Discussionmentioning

confidence: 92%

Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons

Yamanaka

Žitňan

Kim

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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In insects, steroid hormones named ecdysteroids elicit molting and metamorphosis. The prothoracic gland (PG) is a predominant source of ecdysteroids, where their biosynthesis (ecdysteroidogenesis) is regulated by several neuropeptides. Here, we report that FMRFamide-related peptides (FaRPs) regulate ecdysteroidogenesis through direct innervation of the PG in the silkworm Bombyx mori. We purified a previously uncharacterized Bombyx FaRP, DPSFIRFamide, and identified the corresponding Bombyx FMRFamide gene (Bommo-FMRFamide, BRFa), which encodes three additional FaRPs. All BRFa peptides suppressed ecdysteroidogenesis in the PG by reducing cAMP production by means of the receptor for Bommomyosuppressin, another FaRP we have previously shown to act as a prothoracicostatic factor. BRFa is predominantly expressed in neurosecretory cells of thoracic ganglia, and the neurons in the prothoracic ganglion innervate the PG to supply all four peptides to the gland surface. Electrophysiological recordings during development confirmed the increased firing activity of BRFa neurons in stages with low PG activity and decreased ecdysteroid levels in the hemolymph. To our knowledge, this study provides the first report of peptides controlling ecdysteroidogenesis by direct innervation.ecdysteroid ͉ FMRFamide-related peptides ͉ metamorphosis ͉ molting ͉ prothoracic gland

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Section: Discussionmentioning

confidence: 92%

Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons

Yamanaka

Žitňan

Kim

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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“…We first examined some candidate neuropeptide GPCRs based either on the features of Drosophila orthologs (21,22,23) or high expression in the PG, but none of them responded to any PTSPs in our heterologous expression system (Table 1). We then focused on a newly identified Drosophila neuropeptide GPCR, CG16752 (24).…”

Section: Identification and Functional Characterization Of Ptsp-relatedmentioning

confidence: 99%

Bombyx prothoracicostatic peptides activate the sex peptide receptor to regulate ecdysteroid biosynthesis

Yamanaka

Hua

Roller

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

114

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Insect molting and metamorphosis are induced by steroid hormones named ecdysteroids, whose production is regulated by various neuropeptides. We cloned the gene and analyzed the expression of the prothoracicostatic peptide, a unique neuropeptide shown to suppress the production of ecdysteroids in the prothoracic gland of the silkworm, Bombyx mori. We also characterized a Bombyx G proteincoupled receptor, which has previously been identified as an ortholog of the Drosophila sex peptide receptor, as a functional prothoracicostatic peptide receptor. This receptor responded specifically to the prothoracicostatic peptides when examined using a heterologous expression system. The receptor was highly expressed in the prothoracic gland on the day before each larval and pupal ecdysis, when prothoracicostatic peptides are synthesized at a high level in the epiproctodeal glands. These results suggest that the sex peptide receptor functions as a prothoracicostatic peptide receptor in Bombyx and that the peripheral neurosecretory cells as well as the central neuroendocrine system play stage-specific roles in regulating ecdysteroidogenesis.Bombyx mori | G protein-coupled receptor | neuropeptides | steroidogenesis

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“…These data are consistent with the conclusion that DMS acts through two different mechanisms to regulate the fast phase and the slow phase of the cardiac cycle. The D. melanogaster genome database contains two genes encoding predicted myosuppressin receptors; when the genes were expressed the proteins bound DMS [12]. The presence of two myosuppressin receptors in fruitfly is consistent with, but indirect support of the conclusion DMS acts through two different mechanisms in blowfly.…”

Section: Discussionmentioning

confidence: 74%

“…However, there are several experimental parameters that should be considered as a possible basis for the difference; the animals and tissues examined, blowfly heart versus cockroach gut, and types of assay, in vivo versus in vitro, and heart contractions versus stimulation of enzyme release. The in vitro ligand-receptor binding conducted on the expressed myosuppressin proteins did not specifically address the requirement for a C-terminal RFamide [12]. The molecular explanation for this difference in structure requirement awaits further investigations including delineating ligand-receptor binding studies and tissue specificity of the myosuppressin receptors Our immunohistochemical data show DMS may be delivered to the heart and aorta by anterior and posterior dorsal vessel.…”

Section: Discussionmentioning

confidence: 88%

Evidence dromyosuppressin acts at posterior and anterior pacemakers to decrease the fast and the slow cardiac activity in the blowfly Protophormia terraenovae

et al. 2007

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The molecular complexity of the simple blowfly heart makes it an attractive preparation to delineate cardiovascular mechanisms. Blowfly cardiac activity consists of a fast, high frequency signal phase alternating with a slow, low frequency signal phase triggered by pacemakers located in the posterior abdominal heart and anterior thoracocephalic aorta, respectively. Mechanisms underlying FMRFamide-related peptides (FaRPs) effects on heart contractions are not well understood. Here, we report antisera generated to a FaRP, dromyosuppressin (DMS, TDVDHVFLRFamide), recognized neuronal processes that innervated the blowfly Protophormia terraenovae heart and aorta. Dromyosuppressin caused a reversible cardiac arrest. High and low frequency signals were abolished after which they resumed; however, the concentration dependent resumption of the fast phase differed from the slow phase. Dromyosuppressin decreased the frequency of cardiac activity in a dose dependent manner with threshold values between 5 fM and 0.5 fM (fast phase) and 0.5 fM and 0.1 fM (slow phase). Dromyosuppressin structure-activity relationship (SAR) for the decrease of the fast phase frequency was not the same as the SAR for the decrease of the slow phase frequency. The alanyl-substituted analog TDVDHVFLAFamide ([Ala 9 ] DMS) was inactive on the fast phase, but active on the slow phase, a novel finding. FaRPs including myosuppressins are reported to require the C-terminal RFamide for activity. Our data are consistent with the conclusions DMS acts on posterior and anterior cardiac tissue to play a role in regulating the fast and slow phases of cardiac activity, respectively, and ligand-receptor binding requirements of the abdominal and thoracocephalic pacemakers are different.

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Molecular cloning and functional expression of the first two specific insect myosuppressin receptors

Cited by 86 publications

References 37 publications

Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons

Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons

Bombyx prothoracicostatic peptides activate the sex peptide receptor to regulate ecdysteroid biosynthesis

Evidence dromyosuppressin acts at posterior and anterior pacemakers to decrease the fast and the slow cardiac activity in the blowfly Protophormia terraenovae

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