Molecular cloning and functional expression of allergenic sarcoplasmic calcium‐binding proteins from <i>Penaeus</i> shrimps

Mita, Hajime; Koketsu, Aiko; Ishizaki, Shoichiro; Shiomi, Kazuro

doi:10.1002/jsfa.5961

Cited by 23 publications

(16 citation statements)

References 25 publications

(49 reference statements)

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“…Two low molecular weight proteins, SCBP and MLC, are considered crustacean-specific minor allergens [22,23]. Our results showed an IgE binding to SCBP and MLC only in patients with a true shrimp allergy, even if the frequency of sensitization was very low.…”

Section: Discussionmentioning

confidence: 58%

Mite-Induced Asthma and IgE Levels to Shrimp, Mite, Tropomyosin, Arginine Kinase, and Der p 10 Are the Most Relevant Risk Factors for Challenge-Proven Shrimp Allergy

Farioli

Losappio²,

Giuffrida

et al. 2017

Int Arch Allergy Immunol

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Background: Shrimp sensitization is common in the general population, but the presence of symptoms is only moderately related to sensitization. A point still at issue is which in vivo and/or in vitro tests (food challenge, component-resolved diagnosis, house dust mite [HDM] sensitization) can help in distinguishing shrimp-allergic subjects from subjects that are sensitized but tolerant. Methods: The aim of this study was to evaluate the role of IgE to the different shrimp and mite allergens in distinguishing shrimp challenge-positive from challenge-negative patients. Subjects with suspected hypersensitivity reactions to shrimp, positive skin prick tests (SPTs), and/or anti-shrimp IgE were submitted to open and double-blind placebo-controlled food challenges (DBPCFC). Specific IgE to shrimp, mites, and the recombinants rPen a 1, rDer p 1, 2, and 10 were tested using ImmunoCAP-FEIA. IgE immunoblotting was performed to identify the patients' allergenic profiles. Results: In total, 13 out of 51 (25.5%) patients with reported reactions to shrimp were truly shrimpallergic (7 DBPCFC positive and 6 with documented severe reactions). These patients had significantly higher skin test wheal diametersthan nonallergic patients,as well as higher levels of IgE to rPen a 1 and rDer p 10. HDM-induced asthma and thesimultaneous presence of anti-nDer p 1, 2, and 10 IgE levels increased the risk of true shrimp allergy. Conclusion: Food challenge tests are mandatory for the diagnosis of shrimp allergy. Tropomyosin is associated with clinical reactivity. HDM-induced asthma and anti-mite IgE are risk factors for shrimp allergy.

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Section: Discussionmentioning

confidence: 58%

Mite-Induced Asthma and IgE Levels to Shrimp, Mite, Tropomyosin, Arginine Kinase, and Der p 10 Are the Most Relevant Risk Factors for Challenge-Proven Shrimp Allergy

Farioli

Losappio²,

Giuffrida

et al. 2017

Int Arch Allergy Immunol

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“…The black-tiger-shrimp rSCP has been shown to have the same IgE reactivity as the natural counterpart (unfortunately the pacific-white-shrimp rSCP was not assessed). 42 Although SCP and MLC have been reported to be particularly important in children, 13,15,29,43 no strong trend was observed. Differences previously observed might be due to the fact that subjects were not challenged and more adults with negative challenges might have been included.…”

Section: Discussionmentioning

confidence: 97%

Molecular Diagnosis of Shrimp Allergy: Efficiency of Several Allergens to Predict Clinical Reactivity

Pascal

Grishina

Yang

et al. 2015

The Journal of Allergy and Clinical Immunology: In Practice

108

137

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“…Sarcoplasmic Calcium-binding Protein (SCP), was located first in Penaeus monodon (Pen m 4) [44]. Is highly resistant and stable [45].It has high sequence homology with crustacean but low with mollusks [46,47].…”

Section: Sarcoplasmic Calcium-binding Proteinmentioning

confidence: 99%

Shellfish Allergy: Unmet Needs in Diagnosis and Treatment

Gelis¹,

Rueda²,

Valero³

et al. 2020

J Investig Allergol Clin Immunol

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Molecular cloning and functional expression of allergenic sarcoplasmic calcium‐binding proteins from Penaeus shrimps

Abstract: Our results provide further evidence for the high sequence identity among crustacean SCPs. In addition, rSCP will be a useful tool in studying crustacean allergens and also in the diagnosis of crustacean allergy.

Cited by 23 publications

References 25 publications

Mite-Induced Asthma and IgE Levels to Shrimp, Mite, Tropomyosin, Arginine Kinase, and Der p 10 Are the Most Relevant Risk Factors for Challenge-Proven Shrimp Allergy

Mite-Induced Asthma and IgE Levels to Shrimp, Mite, Tropomyosin, Arginine Kinase, and Der p 10 Are the Most Relevant Risk Factors for Challenge-Proven Shrimp Allergy

Molecular Diagnosis of Shrimp Allergy: Efficiency of Several Allergens to Predict Clinical Reactivity

Shellfish Allergy: Unmet Needs in Diagnosis and Treatment

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