Molecular cloning and functional characterization of CvLCYE, a key enzyme in lutein synthesis pathway in Chlorella vulgaris

Lou, Sulin; Lin, Xin; Liu, Chenglong; Anwar, Muhammad; Li, Hui; Hu, Zhangli

doi:10.1016/j.algal.2021.102246

Cited by 16 publications

(15 citation statements)

References 59 publications

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“…Phycobilin pigments are found in cyanobacteria and in the chloroplasts of red algae ( 52 , 65 ). Liutein has a strong antioxidant effect ( 66 ). The main colored compounds in microalgae are fucoxanthin, lutein and β-carotene, and they also are described as good antioxidants ( 58 , 59 , 67 – 69 ).…”

Section: Resultsmentioning

confidence: 99%

Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts

et al. 2022

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Algal biomass (AB) is prospective source of valuable compounds, however, Baltic Sea macroalgae have some challenges, because of their high microbial and chemical contamination. These problems can be solved, by using appropriate technologies for AG pre-treatment. The aim of this study was to evaluate the influence of two pre-treatments, solid-state fermentation with the Lactiplantibacillus plantarum LUHS135 and ultrasonication, on the antioxidant and antimicrobial characteristics of macro- (Cladophora rupestris, Cladophora glomerata, Furcellaria lumbricalis, Ulva intestinalis) and Spirulina (Arthrospira platensis) extracts. Also, combinations of extracts and LUHS135 were developed and their characteristics were evaluated. The total phenolic compound content was determined from the calibration curve and expressed in mg of gallic acid equivalents; antioxidant activity was measured by a Trolox equivalent antioxidant capacity assay using the DPPH• (1,1-diphenyl-2-picrylhydrazyl), ABTS•+ 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), FRAP (Ferric Reducing Ability of Plasma) discoloration methods. Antimicrobial activity was measured by using agar well diffusion assay and in a liquid medium. The highest DPPH• and ABTS•+ was shown by C.rupestris and F.lumbricalis extract × LUHS135 combinations, the highest FRAP - by non-pretreated C.rupestris and F.lumbricalis extract × LUHS135 combinations. Ultrasonicated samples inhibited four out of seven tested pathogens. Finally, the tested pre-treatments showed good perspectives and can be recommended for AB valorization.

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Section: Resultsmentioning

confidence: 99%

Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts

et al. 2022

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“…Elucidation of carotenoids production in bacteria, algae, protist (Patel et al, 2022) and higher plants has progressed significantly, and it is expected that primary carotenoids biosynthesis in these species follows a similar process. Figure 1 depicts the lutein synthesis route in green algae (Chlorophyta), which is subdivided into the different stages (Huang et al, 2021;Kato et al, 2021;Lou et al, 2021;Xie et al, 2021;Song et al, 2022;Velmurugan and Kodiveri Muthukaliannan, 2022). The major metabolic pathway includes the synthesis of isopentenyl diphosphate (IPP), geranylgeranyl pyrophosphate, phytoene, and desaturation of phytoene into lycopene, followed by cyclisation and hydroxylation of lycopene to lutein.…”

Section: Biosynthesis Of Lutein In Microalgae By Native Pathwaymentioning

confidence: 99%

“…Some examples of genetically modified microalgal strains for overproduction of lutein are listed in Table 1. In a study, the lutein content in C. reinhardtii was boosted by improving the biomass Biosynthetic pathway of lutein in microalgae; source references (Huang et al, 2021;Kato et al, 2021;Lou et al, 2021;Xie et al, 2021;Song et al, 2022;Velmurugan and Kodiveri Muthukaliannan, 2022).…”

Section: Genetically Modified Microalgae For Enhanced Production Of L...mentioning

confidence: 99%

Microalgal lutein biosynthesis: Recent trends and challenges to enhance the lutein content in microalgal cell factories

2022

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Photosynthetic organisms such as eukaryotic microalgae and prokaryotic cyanobacteria synthesize a wide range of valuable chemicals. They are predicted to become efficient and renewable sources for valuable products in the future due to their high biomass synthesis using CO2 and solar energy. Microalgae are producers of several carotenoids including lutein, which is a xanthophyll carotenoid with several health advantages, including the prevention of age-related macular degeneration. Currently, it is extracted on commercial scale from marigold flower petals, however, production from plant sources is highly affected by seasonal variations, requires arable land, and has high production cost. Microalgae, on the other hand, are an ideal alternative for lutein synthesis due to their rapid growth and high biomass and lutein yield. It is, however, necessary to further improve lutein productivity, for a successful transition to commercial production. This article describes lutein biosynthesis in microalgae by using their native biochemical pathways, as well as possible target genes for genetic engineering to enhance lutein production. Understanding the processes behind lipid droplet synthesis in chloroplasts, as well as carotenoid transport across chloroplast membranes and carotenoid esterification, might lead to novel ways to boost lutein levels in microalgae.

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“…21 The lutein content in transgenic Chlorella vulgaris expressing lycopene ε-cyclase increased by about 2.3 times compared that of wild type strain. 22 The modified Synechocystis sp. PCC 6803 can accumulate a large amount of lutein by changing the αbranched pathway.…”

Section: Sources and Structure Of Luteinmentioning

confidence: 99%

“…After the major limiting steps of asymmetric ε- and β-cyclization of lycopene were overcome, pathway extension with the proper combination of carotenoid hydroxylases enabled lutein (438 μg/g of dry cells) biosynthesis in Saccharomyces cerevisiae . The lutein content in transgenic Chlorella vulgaris expressing lycopene ε-cyclase increased by about 2.3 times compared that of wild type strain . The modified Synechocystis sp.…”

Section: Sources and Structure Of Luteinmentioning

confidence: 99%

Advances in Research on Natural Distribution, Biosynthesis, Detection, Bioactivity, and Application of Lutein

Zhao

Ci²,

Pang

et al. 2022

ACS Agric. Sci. Technol.

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Lutein, [(3R,3′R,6′R)-β,ε-carotene-3,3′-diol], is a C40 hydroxyl carotenoid. Its chemical structure is based on an unsaturated polyene chain skeleton, and there are one ε-ring and one β-ring on both sides of the skeleton, with a hydroxyl group on the 3′ site of each ring. Lutein is one of the important lipophilic isoprenes from mainly plants and algae. The wide range of sources makes the study of lutein quite comprehensive. The biosynthetic pathway and biochemical activities of lutein have been extensively studied and revealed. Lutein has been extensively studied for its important bioactivity, including for use in functional foods and medicines for brain health, eye-related diseases, skin health, and cancers. This review of lutein summarizes the major studies on bioactivity, metabolism, stability, digestive absorption, lutein delivery systems, and biotechnology.

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Molecular cloning and functional characterization of CvLCYE, a key enzyme in lutein synthesis pathway in Chlorella vulgaris

Cited by 16 publications

References 59 publications

Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts

Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts

Microalgal lutein biosynthesis: Recent trends and challenges to enhance the lutein content in microalgal cell factories

Advances in Research on Natural Distribution, Biosynthesis, Detection, Bioactivity, and Application of Lutein

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