Molecular cloning and functional characterization of duck mitochondrial antiviral-signaling protein (MAVS)

Li, Huilin; Zhai, Yajun; Fan, Yufang; Chen, Huanchun; Zhang, Anding; Jin, Hui; Luo, Rui

doi:10.1016/j.dci.2015.11.004

Cited by 17 publications

(9 citation statements)

References 22 publications

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“…Signaling of duck MAVS was recently shown in duck embryonic fibroblast cells [53], however, transfections were much more reproducible in DF-1 cells. Duck MAVS co-localized and co-precipitated with duck RIG-I CARD domains in DF-1 cells.…”

Section: Discussionmentioning

confidence: 94%

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Influenza PB1-F2 Inhibits Avian MAVS Signaling

Xiao

Evseev

Stevens

et al. 2020

Viruses

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RIG-I plays an essential role in the duck innate immune response to influenza infection. RIG-I engages the critical adaptor protein mitochondrial antiviral signaling (MAVS) to activate the downstream signaling pathway. The influenza A virus non-structural protein PB1-F2 interacts with MAVS in human cells to inhibit interferon production. As duck and human MAVS share only 28% amino acid similarity, it is not known whether the influenza virus can similarly inhibit MAVS signaling in avian cells. Using confocal microscopy we show that MAVS and the constitutively active N-terminal end of duck RIG-I (2CARD) co-localize in DF-1 cells, and duck MAVS is pulled down with GST-2CARD. We establish that either GST-2CARD, or duck MAVS can initiate innate signaling in chicken cells and their co-transfection augments interferon-beta promoter activity. Demonstrating the limits of cross-species interactions, duck RIG-I 2CARD initiates MAVS signaling in chicken cells, but works poorly in human cells. The D122A mutation of human 2CARD abrogates signaling by affecting MAVS engagement, and the reciprocal A120D mutation in duck 2CARD improves signaling in human cells. We show mitochondrial localization of PB1-F2 from influenza A virus strain A/Puerto Rico/8/1934 (H1N1; PR8), and its co-localization and co-immunoprecipitation with duck MAVS. PB1-F2 inhibits interferon-beta promoter activity induced by overexpression of either duck RIG-I 2CARD, full-length duck RIG-I, or duck MAVS. Finally, we show that the effect of PB1-F2 on mitochondria abrogates TRIM25-mediated ubiquitination of RIG-I CARD in both human and avian cells, while an NS1 variant from the PR8 influenza virus strain does not.

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Section: Discussionmentioning

confidence: 94%

“…The average Pearsons's R value of 0.67 indicated moderate co-localization ( Figure 4D). PB1-F2 co-immunoprecipitated with V5-MAVS, but not with another V5-tagged duck protein, the PRY/SPRY domain fragment of duck TRIM27-L [53], suggesting a specific interaction ( Figure 4E).…”

Section: Pr8 Pb1-f2 Interacts With Duck Mavs In the Cytoplasm Of Df-1mentioning

confidence: 99%

Influenza PB1-F2 Inhibits Avian MAVS Signaling

Xiao

Evseev

Stevens

et al. 2020

Viruses

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“…Moreover, this duck protein extracts consists of higher content of Glu and Tyr among their amino acids, highlighting that they possess strong antioxidant activity. At present, a large number of active proteins and peptides with antioxidant effects were isolated and purified from the natural plant and animal resources (Li et al, ; Motoko et al, ; Ren, Wu, Li, Lai, & Xiao, ; Yoko et al, ). Our experimental results suggest that the Cherry Valley ducks with high nutritional value can be effectively used as natural protein resources to protect the human body from oxidative damage of free radicals.…”

Section: Discussionmentioning

confidence: 99%

Image recognition of automatic evisceration of Cherry Valley ducks and biological activities of protein extracts isolated from the duck meat

Cao

Kong

Wang

2018

J Food Process Engineering

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Based on active contour segmentation algorithm and image acquisition system, Cherry Valley duck meats were collected after evisceration, protein extracts were preliminarily isolated, and their various biological properties were evaluated in present study. The amino acid analysis revealed that these protein extracts contained abundant essential amino acids including Thr, Val, Ile, Leu, Met, Phe, Lys, and His, suggesting that this duck meat have high nutritional value. Antioxidant results indicated that the protein extracts had great free radical scavenging effects and excellent in vivo antioxidant capacities. The immunomodulatory results demonstrated that the duck protein extracts exhibited obvious co‐mitogenic effects on the splenocytes activated by the mitogen and effective natural killer (NK) cells' activity. Moreover, the proposed image processing method based on the visceral contour recognition can be applied in poultry processing, which can improve the production efficiency of Cherry Valley duck processing in the poultry industry. Practical applications In the world, duck meat is very popular by people for a long time because of its delicacy and high nutritional value. In the present study, antioxidant and immunomodulatory activities of protein extracts from the Cherry Valley duck show that this duck has significant nutritional value to human body. For a long time, there are many difficult problems on the automatic slaughter and evisceration of duck, chicken and goose in poultry processing industry. Moreover, avian influenza virus has been threatening poultry breeding and slaughter processing, which can easily harm to the health of workers and cause a wide range of virus spread. Image recognition system and contour segmentation algorithm may provide useful information for the development of automatic slaughter of Cherry Valley ducks, which is also helpful to conduct further research in food processing and utilization of various kinds of poultry.

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“…Cloning and characterization of duck MAVS revealed similar domain organization with mammalian MAVS and the closest evolutionary relationship to chicken [123]. Overexpression of MAVS in DEF cells activated NFκB and IRF1 promoters, and MAVS CARD and TM domains were critical for its activity [123]. Finally, poly I:C-induced IFN-β induction was MAVS-dependent, indicating conserved RIG-I-MAVS-TBK1-IRF7 signaling axes [123].…”

Section: Rig-imentioning

confidence: 95%

“…Overexpression of MAVS in DEF cells activated NFκB and IRF1 promoters, and MAVS CARD and TM domains were critical for its activity [123]. Finally, poly I:C-induced IFN-β induction was MAVS-dependent, indicating conserved RIG-I-MAVS-TBK1-IRF7 signaling axes [123].…”

Section: Rig-imentioning

confidence: 99%

Avian Pattern Recognition Receptor Sensing and Signaling

Neerukonda

Katneni

2020

Veterinary Sciences

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Pattern recognition receptors (PRRs) are a class of immune sensors that play a critical role in detecting and responding to several conserved patterns of microorganisms. As such, they play a major role in the maintenance of immune homeostasis and anti-microbial defense. Fundamental knowledge pertaining to the discovery of PRR functions and their ligands continue to advance the understanding of immune system and disease resistance, which led to the rational design and/or application of various PRR ligands as vaccine adjuvants. In addition, the conserved nature of many PRRs throughout the animal kingdom has enabled the utilization of the comparative genomics approach in PRR identification and the study of evolution, structural features, and functions in many animal species including avian. In the present review, we focused on PRR sensing and signaling functions in the avian species, domestic chicken, mallard, and domestic goose. In addition to summarizing recent advances in the understanding of avian PRR functions, the present review utilized a comparative biology approach to identify additional PRRs, whose functions have been well studied in mammalians but await functional characterization in avian.

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Molecular cloning and functional characterization of duck mitochondrial antiviral-signaling protein (MAVS)

Cited by 17 publications

References 22 publications

Influenza PB1-F2 Inhibits Avian MAVS Signaling

Influenza PB1-F2 Inhibits Avian MAVS Signaling

Image recognition of automatic evisceration of Cherry Valley ducks and biological activities of protein extracts isolated from the duck meat

Avian Pattern Recognition Receptor Sensing and Signaling

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