Molecular Cloning and Functional Characterization of a Salt Tolerance-Associated Gene IbNFU1 from Sweetpotato

Wang, Lianjun; He, Shao-zhen; Zhai, Hong; Liu, De-gao; Wang, Yannan; Liu, Qingchang

doi:10.1016/s2095-3119(13)60202-6

Cited by 26 publications

(18 citation statements)

References 43 publications

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“…As a source of bioenergy, sweet potato is mainly planted on marginal land; hence, improving its salt tolerance is important to maintain productivity [26,27]. Until now, a series of salttolerance-associated genes, such as IbOr, IbNFU1, IbP5CR, IbMas, IbSIMT1, IbMIPS1 and IbZFP1, has already been well characterised in sweet potatoes [26,[28][29][30][31][32][33][34][35]. However, very little work has been done on sweet potato miRNAs, and miRNA-related salt stress in sweet potato has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

High-throughput deep sequencing reveals the important role that microRNAs play in the salt response in sweet potato (Ipomoea batatas L.)

et al. 2020

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Background: MicroRNAs (miRNAs), a class of small regulatory RNAs, have been proven to play important roles in plant growth, development and stress responses. Sweet potato (Ipomoea batatas L.) is an important food and industrial crop that ranks seventh in staple food production. However, the regulatory mechanism of miRNAmediated abiotic stress response in sweet potato remains unclear. Results: In this study, we employed deep sequencing to identify both conserved and novel miRNAs from salinityexposed sweet potato cultivars and its untreated control. Twelve small non-coding RNA libraries from NaCl-free (CK) and NaCl-treated (Na150) sweet potato leaves and roots were constructed for salt-responsive miRNA identification in sweet potatoes. A total of 475 known miRNAs (belonging to 66 miRNA families) and 175 novel miRNAs were identified. Among them, 51 (22 known miRNAs and 29 novel miRNAs) were significantly up-regulated and 76 (61 known miRNAs and 15 novel miRNAs) were significantly down-regulated by salinity stress in sweet potato leaves; 13 (12 known miRNAs and 1 novel miRNAs) were significantly up-regulated and 9 (7 known miRNAs and 2 novel miRNAs) were significantly down-regulated in sweet potato roots. Furthermore, 636 target genes of 314 miRNAs were validated by degradome sequencing. Deep sequencing results confirmed by qRT-PCR experiments indicated that the expression of most miRNAs exhibit a negative correlation with the expression of their targets under salt stress. Conclusions:This study provides insights into the regulatory mechanism of miRNA-mediated salt response and molecular breeding of sweet potatoes though miRNA manipulation.

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Section: Introductionmentioning

confidence: 99%

High-throughput deep sequencing reveals the important role that microRNAs play in the salt response in sweet potato (Ipomoea batatas L.)

et al. 2020

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“…The BADH gene from spinach improved tolerance to salt, MV‐mediated oxidative and low temperature stresses in transgenic sweet potato (Fan et al ., ). Overexpression of the genes, IbOr , IbLCY‐ ε, IbNFU1 , IbP5CR , IbMas and IbSIMT1 , from sweet potato enhanced salt tolerance of this crop (Kim et al ., ,b; Liu et al ., ,b,c, ; Wang et al ., ).…”

Section: Introductionmentioning

confidence: 99%

A myo‐inositol‐1‐phosphate synthase gene, IbMIPS1, enhances salt and drought tolerance and stem nematode resistance in transgenic sweet potato

Zhai

Wang

et al. 2015

Plant Biotechnology Journal

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177

138

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Summary Myo‐inositol‐1‐phosphate synthase (MIPS) is a key rate limiting enzyme in myo‐inositol biosynthesis. The MIPS gene has been shown to improve tolerance to abiotic stresses in several plant species. However, its role in resistance to biotic stresses has not been reported. In this study, we found that expression of the sweet potato IbMIPS1 gene was induced by NaCl, polyethylene glycol (PEG), abscisic acid (ABA) and stem nematodes. Its overexpression significantly enhanced stem nematode resistance as well as salt and drought tolerance in transgenic sweet potato under field conditions. Transcriptome and real‐time quantitative PCR analyses showed that overexpression of IbMIPS1 up‐regulated the genes involved in inositol biosynthesis, phosphatidylinositol (PI) and ABA signalling pathways, stress responses, photosynthesis and ROS‐scavenging system under salt, drought and stem nematode stresses. Inositol, inositol‐1,4,5‐trisphosphate (IP3), phosphatidic acid (PA), Ca2+, ABA, K+, proline and trehalose content was significantly increased, whereas malonaldehyde (MDA), Na+ and H2O2 content was significantly decreased in the transgenic plants under salt and drought stresses. After stem nematode infection, the significant increase of inositol, IP3, PA, Ca2+, ABA, callose and lignin content and significant reduction of MDA content were found, and a rapid increase of H2O2 levels was observed, peaked at 1 to 2 days and thereafter declined in the transgenic plants. This study indicates that the IbMIPS1 gene has the potential to be used to improve the resistance to biotic and abiotic stresses in plants.

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“…The overexpression of IbOr and IbLCY-e genes increased carotenoid accumulation and salt tolerance in transgenic sweetpotato calluses (Kim et al 2013a, b). Wang et al (2013a) isolated IbNFU1 gene from sweetpotato and Liu et al (2014b) found this gene was involved in salt tolerance of sweetpotato. Liu et al (2014a) cloned IbP5CR gene from sweetpotato and the IbP5CR-overexpressing sweetpotato plants exhibited higher salt tolerance.…”

Section: Introductionmentioning

confidence: 99%

IbSIMT1, a novel salt-induced methyltransferase gene from Ipomoea batatas, is involved in salt tolerance

Liu

Song

et al. 2014

Plant Cell Tiss Organ Cult

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S-adenosyl-methionine (SAM)-dependent methyltransferase (MTase) genes are a multigene family; however, only a few have been characterized at the functional level. In the present study, a novel salt-induced SAM-dependent MTase gene, named IbSIMT1, was isolated from salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line ND98. IbSIMT1 contains a DUF248 domain of unknown function and an MTase domain. Expression of IbSIMT1 was up-regulated in sweetpotato under salt stress and abscisic acid treatment. The IbSIMT1-overexpressing sweetpotato (cv. Shangshu 19) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content was significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H 2 O 2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbSIMT1 up-regulated the salt stress responsive genes, including pyrroline-5-carboxylate synthase, pyrroline-5-carboxylate reductase, SOD, psbA and phosphoribulokinase genes under salt stress.These findings suggest that the novel IbSIMT1 gene is involved in sweetpotato salt tolerance and enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and increasing reactive oxygen species scavenging capacity.

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Molecular Cloning and Functional Characterization of a Salt Tolerance-Associated Gene IbNFU1 from Sweetpotato

Cited by 26 publications

References 43 publications

High-throughput deep sequencing reveals the important role that microRNAs play in the salt response in sweet potato (Ipomoea batatas L.)

High-throughput deep sequencing reveals the important role that microRNAs play in the salt response in sweet potato (Ipomoea batatas L.)

A myo‐inositol‐1‐phosphate synthase gene, IbMIPS1, enhances salt and drought tolerance and stem nematode resistance in transgenic sweet potato

IbSIMT1, a novel salt-induced methyltransferase gene from Ipomoea batatas, is involved in salt tolerance

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