Molecular Cloning and Functional Characterization of Heat Stress-Responsive Superoxide Dismutases in Garlic (Allium sativum L.)

Ji, Hyo Seong; Bang, Seoung Gun; Ahn, Mina; Kim, Gayeon; Kim, Eunhui; Eom, Seung Hee; Hyun, Tae Kyung

doi:10.3390/antiox10050815

Cited by 13 publications

(7 citation statements)

References 31 publications

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“…The phenological stage of a species has an optimal temperature range for normal growth and development. Any deviation (low or high) from this range either activates tolerance mechanisms or damages the plant (Ji et al 2021). At the morphological level, damage as a result of temperature stress is manifested as inhibition of root and shoot growth, leaf scorching/abscission, stem burning, and fruit discoloration, ultimately decreasing crop yield (Chaudhary et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Assessment of proline function in higher plants under extreme temperatures

et al. 2023

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Climate change and abiotic stress factors are key players in crop losses worldwide. Among which, extreme temperatures (heat and cold) disturb plant growth and development, reduce productivity and, in severe cases, lead to plant death. Plants have developed numerous strategies to mitigate the detrimental impact of temperature stress. Exposure to stress leads to the accumulation of various metabolites, e.g. sugars, sugar alcohols, organic acids and amino acids. Plants accumulate the amino acid 'proline' in response to several abiotic stresses, including temperature stress. Proline abundance may result from de novo synthesis, hydrolysis of proteins, reduced utilization or degradation. Proline also leads to stress tolerance by maintaining the osmotic balance (still controversial), cell turgidity and indirectly modulating metabolism of reactive oxygen species. Furthermore, the crosstalk of proline with other osmoprotectants and signalling molecules, e.g. glycine betaine, abscisic acid, nitric oxide, hydrogen sulfide, soluble sugars, helps to strengthen protective mechanisms in stressful environments. Development of less temperature-responsive cultivars can be achieved by manipulating the biosynthesis of proline through genetic engineering. This review presents an overview of plant responses to extreme temperatures and an outline of proline metabolism under such temperatures. The exogenous application of proline as a protective molecule under extreme temperatures is also presented. Proline crosstalk and interaction with other molecules is also discussed. Finally, the potential of genetic engineering of proline-related genes is explained to develop 'temperature-smart' plants. In short, exogenous application of proline and genetic engineering of proline genes promise ways forward for developing 'temperature-smart' future crop plants.

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Section: Introductionmentioning

confidence: 99%

Assessment of proline function in higher plants under extreme temperatures

et al. 2023

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“…When aquatic organisms are exposed to stressful environment, their bodies generate amounting ROS against it. Although ROS are bene cial for host against stress, overmuch ROS contribute to some damages like damage of DNA, proteins, lipid peroxidation and some diseases [5,38,39]. SOD family is the rst line to resist oxidative [40], which plays an important role to remove ROS for invertebrates.…”

Section: Discussionmentioning

confidence: 99%

An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

Feng¹,

Gao²,

Kong³

et al. 2022

Fish & Shellfish Immunology

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“…The accumulation of H 2 O 2 in N . benthamiana leaves was carried out using the 3,3-diaminobenzidine (DAB) staining method, as described by Ji et al [ 18 ]. In addition, MDA content was analyzed as described by Eom et al [ 13 ] and expressed as nmol/mg of F.W.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of the Brassica rapa bZIP Transcription Factor, BrbZIP-S, Increases the Stress Tolerance in Nicotiana benthamiana

Eom

Lim

Hyun

2023

Biology

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In higher plants, S1-basic region-leucine zipper (S1-bZIP) transcription factors fulfill crucial roles in the physiological homeostasis of carbon and amino acid metabolisms and stress responses. However, very little is known about the physiological role of S1-bZIP in cruciferous vegetables. Here, we analyzed the physiological function of S1-bZIP from Brassica rapa (BrbZIP-S) in modulating proline and sugar metabolism. Overexpression of BrbZIP-S in Nicotiana benthamiana resulted in delayed chlorophyll degradation during the response to dark conditions. Under heat stress or recovery conditions, the transgenic lines exhibited a lower accumulation of H2O2, malondialdehyde, and protein carbonyls compared to the levels in transgenic control plants. These results strongly indicate that BrbZIP-S regulates plant tolerance against dark and heat stress. We propose that BrbZIP-S is a modulator of proline and sugar metabolism, which are required for energy homeostasis in response to environmental stress conditions.

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Molecular Cloning and Functional Characterization of Heat Stress-Responsive Superoxide Dismutases in Garlic (Allium sativum L.)

Cited by 13 publications

References 31 publications

Assessment of proline function in higher plants under extreme temperatures

Assessment of proline function in higher plants under extreme temperatures

An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

Overexpression of the Brassica rapa bZIP Transcription Factor, BrbZIP-S, Increases the Stress Tolerance in Nicotiana benthamiana

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