Molecular cloning and functional characterization of caspase 9 in large yellow croaker (Pseudosciaena crocea)

Molecular characterization of a Toll-like receptor 22 homologue in large yellow croaker (Pseudosciaena crocea) and promoter activity analysis of its 5′-flanking sequence

“…To obtain the full-length cDNA of LycTLR22, 5 0 and 3 0 RACE-PCR was performed using 5 0 -Full RACE Kit and 3 0 -Full RACE Core Set (TaKaRa).The detailed protocol is as previously described [28]. Briefly, primers for the 5 0 and 3 0 RACE were designed based on the partial cDNA sequence obtained above.…”

“…Genomic DNA of large yellow croaker was prepared as described previously [28]. The primer set of P5 and P6 was designed against the full-length cDNA sequence of LycTLR22 (Table 1).…”

Molecular cloning and characterization of caspase-3 in large yellow croaker (Pseudosciaena crocea)

“…Recombinant Lyccasp3 excluding the prodomain was expressed as a 6 Â His-tagged fusion protein and purified as described previously [1]. Briefly, Lyccasp3 gene fragment encoding residues 38e285 was amplified using the primer set Casp3-Exp-F1 and Casp3-Exp-R1 (Table 1), and cloned into pET-His vector (Gene Power Lab, China).…”

“…Recently, Mu et al [1] reported that caspase-9, a key component of intrinsic apoptotic pathway, was involved in the immune response induced by poly(I:C) or bacterial vaccine in large yellow croaker. Caspase-3 is activated by caspase-9 and plays a role as one of the executioners in the intrinsic apoptotic pathway [16].…”

Characterization of a RacGTPase up-regulated in the large yellow croaker Pseudosciaena crocea immunity

“…The cellular immune responses include encapsulation, phagocytosis and nodule formation, whereas the humoral responses include the clotting cascade, the synthesis of a wide array of anti-microbial peptides and the phenoloxidase (PO)-activating system [4]. Up to date, some genes, like Ran [5], MyD88 [6], IRF1 [7], Toll9 [8], CXCL13 [9], Caspase 9 [10] and interferon transmembrane protein1 [11] involved in innate immunity of large yellow croaker have been characterized.…”