Molecular cloning and functional analysis of a sugar transporter gene (<i>CsTST2</i>) from cucumber (<i>Cucumis sativus</i> L.)

Hu, Bin; Huang, Weifeng; Dong, Liaoliao; Liu, Shiqiang; Zhou, Yong

doi:10.1080/13102818.2018.1555011

Cited by 11 publications

(12 citation statements)

References 48 publications

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“…Source-to-sink transport of sugars can modify the carbohydrate distribution and homeostasis that contribute to the tolerance of plants to abiotic stresses [54,72]. Many SWEET genes were regulated by various abiotic stresses, suggesting that they may play regulatory roles in the responses to various abiotic stresses by controlling sugar allocation.…”

Section: Discussionmentioning

confidence: 99%

“…The qRT-PCR reactions were performed in 20 μL reactions using 1 μL of cDNA, with the FastStar Universal SYBR Green Master (ROX) (Roche Diagnostics, Indianapolis, IN, USA), on the ABI 7500 system (Thermo Fisher Scientific, Waltham, MA, USA). The qRT-PCR conditions were as described previously [54]. Three independent biological replicates were conducted.…”

Section: Methodsmentioning

confidence: 99%

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SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis

Huang

et al. 2019

Plants

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SWEET (Sugars Will Eventually be Exported Transporter) proteins mediate the translocation of sugars across cell membranes and play crucial roles in plant growth and development as well as stress responses. In this study, a total of 25 SWEET genes were identified from the Medicago truncatula genome and were divided into four clades based on the phylogenetic analysis. The MtSWEET genes are distributed unevenly on the M. truncatula chromosomes, and eight and 12 MtSWEET genes are segmentally and tandemly duplicated, respectively. Most MtSWEET genes contain five introns and encode proteins with seven transmembrane helices (TMHs). Besides, nearly all MtSWEET proteins have relatively conserved membrane domains, and contain conserved active sites. Analysis of microarray data showed that some MtSWEET genes are specifically expressed in disparate developmental stages or tissues, such as flowers, developing seeds and nodules. RNA-seq and qRT-PCR expression analysis indicated that many MtSWEET genes are responsive to various abiotic stresses such as cold, drought, and salt treatments. Functional analysis of six selected MtSWEETs in yeast revealed that they possess diverse transport activities for sucrose, fructose, glucose, galactose, and mannose. These results provide new insights into the characteristics of the MtSWEET genes, which lay a solid foundation for further investigating their functional roles in the developmental processes and stress responses of M. truncatula.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis

Huang

et al. 2019

Plants

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“…The authors observed that salt stress modulated the expression of SUCROSE SYNTHASE ( SUSY1 ), and different sugar transporters such as TMT and SWEET and, also induced alterations in the vascular anatomy of the inflorescence stem (Sellami et al, 2019). Hu et al (2019) identified 25 SWEET genes in the Medicago truncatula genome and reported that half of the MtSWEET genes demonstrated significant transcript accumulation in cold, salt and drought stresses. Besides this, the transcript levels of seven genes including MtSWEET1a , 2b , 3c , 9b , 13 , 15c , and 16 are significantly altered by drought, salt, and cold treatments (Hu et al, 2019).…”

Section: The Interplay Between Sugars and Phytohormones Under Abiotic Stressmentioning

confidence: 99%

Plant sugars: Homeostasis and transport under abiotic stress in plants

Saddhe

Manuka

Suprasanna

2020

Physiologia Plantarum

194

105

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The sessile nature of plants' life is endowed with a highly evolved defense system to adapt and survive under environmental extremes. To combat such stresses, plants have developed complex and well‐coordinated molecular and metabolic networks encompassing genes, metabolites, and acclimation responses. These modulate growth, photosynthesis, osmotic maintenance, and carbohydrate homeostasis. Under a given stress condition, sugars act as key players in stress perception, signaling, and are a regulatory hub for stress‐mediated gene expression ensuring responses of osmotic adjustment, scavenging of reactive oxygen species, and maintaining the cellular energy status through carbon partitioning. Several sugar transporters are known to regulate carbohydrate partitioning and key signal transduction steps involved in the perception of biotic and abiotic stresses. Sugar transporters such as SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTER (SWEETs), SUCROSE TRANSPORTERS (SUTs), and MONOSACCHARIDE TRANSPORTERS (MSTs) are involved in sugar loading and unloading as well as long‐distance transport (source to sink) besides orchestrating oxidative and osmotic stress tolerance. It is thus necessary to understand the structure–function relationship of these sugar transporters to fine‐tune the abiotic stress‐modulated responses. Advances in genomics have unraveled many sugars signaling components playing a key role in cross‐talk in abiotic stress pathways. An integrated omics approach may aid in the identification and characterization of sugar transporters that could become targets for developing stress tolerance plants to mitigate climate change effects and improve crop yield. In this review, we have presented an up‐to‐date analysis of the sugar homeostasis under abiotic stresses as well as describe the structure and functions of sugar transporters under abiotic stresses.

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“…Unlike all other plants, in the Solanaceae family, type III SUTs (SUT4 here) have been localized to the plasma membrane instead of the tonoplast (Chincinska et al, 2008). A tonoplast sugar transporter (TST), CsTST2 from Cucumis sativus (cucumber), was also found to be upregulated during various abiotic stresses like salt, drought, and cold and was proposed to import fructose, galactose, mannose, and sucrose inside the vacuoles (Hu et al, 2019). Even though potato SUT4 has been characterized to be a highly efficient sucrose transporter, its decreased expression during drought stress indicated that it is unlikely to be directly involved in phloem loading.…”

Section: Sugar Status Impacts Source‐sink Relationship During Droughtmentioning

confidence: 99%

Imperative role of sugar signaling and transport during drought stress responses in plants

Kaur

Manna

Thakur

et al. 2021

Physiologia Plantarum

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Cellular sugar status is essentially maintained during normal growth conditions but is impacted negatively during various environmental perturbations. Drought presents one such unfavorable environmental cue that hampers the photosynthetic fixation of carbon into sugars and affects their transport by lowering the cellular osmotic potential. The transport of cellular sugar is facilitated by a specific set of proteins known as sugar transporters. These transporter proteins are the key determinant of influx/ efflux of various sugars and their metabolite intermediates that support the plant growth and developmental process. Abiotic stress and especially drought stress‐mediated injury results in reprogramming of sugar distribution across the cellular and subcellular compartments. Here, we have reviewed the imperative role of sugar accumulation, signaling, and transport under typical and atypical stressful environments. We have discussed the physiological effects of drought on sugar accumulation and transport through different transporter proteins involved in monosaccharide and disaccharide sugar transport. Further, we have illustrated sugar‐mediated signaling and regulation of sugar transporter proteins along with the overall crosstalk of this signaling with the phytohormone module of abiotic stress response under osmotic stress. Overall, the present review highlights the critical role of sugar transport, distribution and signaling in plants under drought stress conditions.

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Molecular cloning and functional analysis of a sugar transporter gene (CsTST2) from cucumber (Cucumis sativus L.)

Abstract: View related articles View Crossmark data Citing articles: 4 View citing articles Molecular cloning and functional analysis of a sugar transporter gene (CsTST2) from cucumber (Cucumis sativus L.

Cited by 11 publications

References 48 publications

SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis

SWEET Gene Family in Medicago truncatula: Genome-Wide Identification, Expression and Substrate Specificity Analysis

Plant sugars: Homeostasis and transport under abiotic stress in plants

Imperative role of sugar signaling and transport during drought stress responses in plants

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