Molecular cloning and functional analysis of zebrafish high-density lipoprotein-binding protein

Chen, Jyh-Yih; Chen, Jian-Chyi; Wu, Jen‐Leih

doi:10.1016/s1096-4959(03)00181-7

Cited by 53 publications

(23 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All the primer sets, with the exception of C1r/s and MASP, were designed on the basis of the conserved regions of the molecules to enable recognition of all the described isoforms of each gene. b-actin has been shown to be a validated reference gene for time course studies in D. rerio embryogenesis [16], and was therefore selected as a reference to standardize the results by eliminating variations in mRNA and cDNA quantity and quality [17]. Each primer set was checked on a zebrafish liver cDNA for its effectiveness in amplifying the desired fragment ( Fig.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Response of complement expression to challenge with lipopolysaccharide in embryos/larvae of zebrafish Danio rerio: Acquisition of immunocompetent complement

Wang

Zhang

Wang

2008

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Response of complement expression to challenge with lipopolysaccharide in embryos/larvae of zebrafish Danio rerio: Acquisition of immunocompetent complement

Wang

Zhang

Wang

2008

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…Oligonucleotide primer pairs of Vtg I, Vtg II, ERα and housekeeping genes were used (Table 1). β-actin transcript was used to standardize the results by eliminating variations in mRNA and cDNA quantity and quality (Chen et al 2003). Each mRNA level was expressed as its ratio to β-actin mRNA.…”

Section: Real-time Quantitative Pcrmentioning

confidence: 99%

Hepatic and extrahepatic expression of estrogen-responsive genes in male adult zebrafish (Danio rerio) as biomarkers of short-term exposure to 17β-estradiol

Jin

Wang

Sheng

et al. 2007

Environ Monit Assess

View full text Add to dashboard Cite

Growing concern over reproductive hormones in the environment demands sensitive and efficient methods by use of molecular biomarkers to detect these contaminants in oviparous vertebrates. In this study, a real-time quantitative RT-PCR was adopted to investigate the expressions of three estrogen-responsive genes, Vtg I, Vtg II and ERalpha, in hepatic and extrahepatic tissues of male adult zebrafish exposed to varying concentrations of 17beta-estradiol (E2) for selected periods. Without exposure to E2, all the genes were expressed in the tissues of male controls with their levels being much lower than those in the respective tissues of female controls. The expressions of hepatic Vtg I and II mRNAs were induced significantly after 1-day exposure to E2 at as low as 0.25 microg L(-1). Significant induction in the expression of hepatic ERalpha mRNA required a higher E2 concentration (> or = 0.5 microg L(-1)) and a longer exposure (> or = 2 days), suggesting that Vtg I and Vtg II are more sensitive to E2 exposure. The induction of Vtg mRNA in the skin of zebrafish was also significant following a short exposure (1 day) to low E2 concentration (0.25 microg L(-1)), with the levels of Vtg I and Vtg II mRNA being increased by 25 and 5 times, respectively. These results suggest that Vtg I mRNA is a highly sensitive biomarker for determining the estrogenic effects of E2 and that the skin of zebrafish may be an appropriate substitute for liver for such a determination.

show abstract

“…3e) and contributed to significantly differentiate fish in LSP-S from the other sites. HDLBP plays an important role in endocrine regulation of both lipids and cholesterol by mediating the reverse transport of cholesterol from peripheral tissues to the liver and was suggested to play a role in the efficiency of fish intake of food and lipid transfer (Chen et al 2003). Transcription results may therefore suggest an impairment of lipid metabolism and regulation in yellow perch from LSP and could partly account for the reduced general body condition measured in fish at this lake in the first part of this study (Giraudo et al 2016).…”

Section: Energy Metabolismmentioning

confidence: 70%

Integrated spatial health assessment of yellow perch (Perca flavescens) populations from the St. Lawrence River (QC, Canada), part B: cellular and transcriptomic effects

Bruneau

Landry

Giraudo

et al. 2016

Environ Sci Pollut Res

View full text Add to dashboard Cite

Multi-biological level assessments have become great tools to evaluate the health of aquatic ecosystems. Using this approach, a complementary study was designed to evaluate the health of yellow perch (Perca flavescens) populations in the St. Lawrence River (Quebec, Canada). In the present study, stress responses were compared at the transcriptomic, cellular, and tissue levels in yellow perch collected at six sites along the river: Lake St. François, Lake St. Louis (north and south), Beauregard Island and Lake St. Pierre (north and south). These results complement the physiological and chemical parameters as well as pathogen infection investigated in a companion paper published in the present issue. Thiobarbituric acid reactive substance (TBARS) analyses indicated the presence of oxidative stress in fish collected in the southern part of Lake St. Louis and at the downstream sites of Lake St. Pierre. High lipid peroxidation levels were found in the muscle of yellow perch caught at Beauregard Island, located downstream of the Montreal's wastewater treatment plant, suggesting an impact of the municipal effluent on redox homeostasis. Transcriptomic results indicated the down-regulation of genes related to lipid, glucose, and retinoid in southern Lake St. Pierre as well as a decrease in retinoid storage. Overall, biochemical and molecular markers indicated that the health status of yellow perch followed a decreasing gradient from upstream to downstream of the St. Lawrence River. This gradient is representative of the cumulative negative impacts of human activities on water and habitat quality along the river.

show abstract

Molecular cloning and functional analysis of zebrafish high-density lipoprotein-binding protein

Cited by 53 publications

References 34 publications

Response of complement expression to challenge with lipopolysaccharide in embryos/larvae of zebrafish Danio rerio: Acquisition of immunocompetent complement

Response of complement expression to challenge with lipopolysaccharide in embryos/larvae of zebrafish Danio rerio: Acquisition of immunocompetent complement

Hepatic and extrahepatic expression of estrogen-responsive genes in male adult zebrafish (Danio rerio) as biomarkers of short-term exposure to 17β-estradiol

Integrated spatial health assessment of yellow perch (Perca flavescens) populations from the St. Lawrence River (QC, Canada), part B: cellular and transcriptomic effects

Contact Info

Product

Resources

About