Molecular Cloning and Functional Analysis of Three Type D Endogenous Retroviruses of Sheep Reveal a Different Cell Tropism from That of the Highly Related Exogenous Jaagsiekte Sheep Retrovirus

Palmarini, Massimo; Hallwirth, Claus V.; York, Denis; Murgia, Claudio; Oliveira, Túlio de; Spencer, Thomas E.; Fan, Hung

doi:10.1128/jvi.74.17.8065-8076.2000

Cited by 106 publications

(187 citation statements)

References 66 publications

(51 reference statements)

Supporting

Mentioning

175

Contrasting

Unclassified

Order By: Relevance

“…Owing to the short length of the enJSRV copies amplified experimentally in this work, we decided to map them by using whole nucleotide sequences of previously sequenced enJSRVs (GenBank accession numbers: AF136224, AF136225, AF153615 and EF680296-319) (Palmarini et al, 2000;Arnaud et al, 2007). This strategy was based on the similarity of sequences, and previously sequenced enJSRVs were used as a search query.…”

Section: Bioinformatic Analysesmentioning

confidence: 99%

“…We followed the nomenclature used by Palmarini et al (2000) and Arnaud et al (2007) for the previously characterized enJSRVs. The two major clades previously identified by Palmarini et al (2000) and designed enJSRV-A and enJSRV-B were used to describe the two main phylogenetic clusters .…”

Section: Enjsrv Nomenclature and Classificationmentioning

confidence: 99%

“…The two major clades previously identified by Palmarini et al (2000) and designed enJSRV-A and enJSRV-B were used to describe the two main phylogenetic clusters . The most ancient enJSRV copies belong to the enJSRV-A group and recent enJSRV copies (including polymorphic enJSRVs) are clustered into the enJSRV-B group.…”

Section: Enjsrv Nomenclature and Classificationmentioning

confidence: 99%

“…These LTR primers were designed using conserved regions of previously sequenced enJSRV LTRs in sheep (GenBank accession numbers: AF136224, AF136225, AF153615 and EF680296-319) (Palmarini et al, 2000;Arnaud et al, 2007). Primer sequences and locations are shown in Figure 1.…”

Section: Experimental Detection Of Enjsrvsmentioning

confidence: 99%

See 3 more Smart Citations

Evolutionary dynamics of endogenous Jaagsiekte sheep retroviruses proliferation in the domestic sheep, mouflon and Pyrenean chamois

Sistiaga-Poveda

Jugo

2014

Heredity

View full text Add to dashboard Cite

The oncogenic exogenous Jaagsiekte sheep retrovirus (JSRV), responsible for ovine pulmonary adenocarcinoma, has several endogenous counterparts termed enJSRVs. Although many of these elements have been inactivated over time by the accumulation of deleterious mutations or internal recombination leading to solo long terminal repeat (LTR) formation, several members of enJSRVs have been identified as nearly intact and probably represent recent integration events. To determine the level of enJSRV polymorphism in the sheep population and related species, we have undertaken a study by characterizing enJSRVs copies and independent integration sites in six domestic sheep and two wild species of the sheep lineage. enJSRVs copies were detected by amplifying the env-LTR region by PCR, and for the detection of the insertion sites, we used two approaches: (1) an in silico approach based on the recently published Sheep Reference Genome Assembly (OARv3.0) and (2) an experimental approach based on PCR suppression and inverse PCR techniques. In total, 103 enJSRV sequences were generated across 10 individuals and enJSRV integrations were found on 11 of the 28 sheep chromosomes. These findings suggest that there are still uncharacterized enJSRVs, and that some of the integration sites are variable among the different species, breeds of the same species, subspecies and geographic locations.

show abstract

Section: Bioinformatic Analysesmentioning

confidence: 99%

Section: Enjsrv Nomenclature and Classificationmentioning

confidence: 99%

Section: Enjsrv Nomenclature and Classificationmentioning

confidence: 99%

Section: Experimental Detection Of Enjsrvsmentioning

confidence: 99%

See 2 more Smart Citations

Evolutionary dynamics of endogenous Jaagsiekte sheep retroviruses proliferation in the domestic sheep, mouflon and Pyrenean chamois

Sistiaga-Poveda

Jugo

2014

Heredity

View full text Add to dashboard Cite

show abstract

“…Results from the previous experiment suggested that the LTRs are not entirely responsible for the cell-type-specific oncogenicity observed in naturally infected sheep and that perhaps other regions of the ovine betaretroviral genome contribute to the disease spectrum. The genomic sequences of JSRV and ENTV differ at the 3Ј end of the env gene, just upstream of the 3Ј LTR (29), in a region that has been shown to be dispensable for transformation (17), but possesses enhancer functions in other retroviruses (3,18). To test the potential role of this region in determining disease tropism, we incorporated the ϳ75-bp sequence upstream of the JSRV and ENTV-1 3Ј LTRs into AJAP and AE 1 AP to create A JE JAP and A EE E 1 AP, where JE stands for JSRV element and EE stands for ENTV element (Fig.…”

Section: Resultsmentioning

confidence: 99%

Jaagsiekte Sheep Retrovirus and Enzootic Nasal Tumor Virus Promoters Drive Gene Expression in All Airway Epithelial Cells of Mice but Only Induce Tumors in the Alveolar Region of the Lungs

Linnerth-Petrik

Halbert

et al. 2011

J Virol

View full text Add to dashboard Cite

Jaagsiekte sheep retrovirus (JSRV) induces tumors in the distal airways of sheep and goats, while the closely related enzootic nasal tumor virus type 1 (ENTV-1) and ENTV-2 induce tumors in the nasal epithelium of sheep and goats, respectively. When expressed using a strong Rous sarcoma virus promoter, the envelope proteins of these viruses induce tumors in the respiratory tract of mice, but only in the distal airway. To examine the role of the retroviral long terminal repeat (LTR) promoters in determining tissue tropism, adeno-associated virus (AAV) vectors expressing alkaline phosphatase under the control of the JSRV, ENTV-1, or ENTV-2 LTRs were generated and administered to mice. The JSRV LTR was active in all airway epithelial cells, while the ENTV LTRs were active in the nasal epithelium and alveolar type II cells but poorly active in tracheal and bronchial epithelial cells. When vectors were administered systemically, the ENTV-1 and -2 LTRs were inactive in major organs examined, whereas the JSRV showed high-level activity in the liver. When a putative transcriptional enhancer from the 3 end of the env gene was inserted upstream of the JSRV and ENTV-1 LTRs in the AAV vectors, a dramatic increase in transgene expression was observed. However, intranasal administration of AAV vectors containing any combination of ENTV or JSRV LTRs and Env proteins induced tumors only in the lower airway. Our results indicate that mice do not provide an adequate model for nasal tumor induction by ENTV despite our ability to express genes in the nasal epithelium.Jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus (ENTV) are closely related oncogenic betaretroviruses that infect sheep and goats. These viruses share greater than 90% sequence identity and utilize the same cellular receptor for virus attachment and entry (10, 32), yet they cause distinctly different diseases. JSRV transforms secretory epithelial cells of the distal lung leading to ovine pulmonary adenocarcinoma (OPA) (28,34). ENTV also transforms secretory epithelial cells, although in this case the target cells are located in the nasal mucosa and lead to the formation of a neoplastic disease called enzootic nasal adenocarcinoma (ENA) (9). Two distinct viruses have been implicated in the etiology of ENA, one in sheep (ENTV-1) and one in goats (ENTV-2), and the genomic sequences of both have been determined (7,26,38).It is currently not known how such different diseases result from infection by these closely related retroviruses. Experimental induction of OPA in sheep has been demonstrated using a molecular clone of JSRV (30); however, the lack of a cell culture system capable of propagating ENTV, the fact that an infectious molecular clone of ENTV has not yet been published, and the limitation that experiments using sheep are expensive, have made it difficult to study the mechanism of disease tropism in sheep. Therefore, we developed a model to study JSRV and ENTV tumorigenesis in mice using adenoassociated virus (AAV) vectors (41). AAV vectors made w...

show abstract

Oncogenesis by retroviruses: old and new paradigms

Maeda

Fan

Yoshikai

2008

Reviews in Medical Virology

Self Cite

View full text Add to dashboard Cite

Retroviruses are associated with a variety of diseases including an array of malignancies, immunodeficiencies and neurological disorders. In particular, studies of oncogenic retroviruses established fundamental principles of modern molecular cancer biology. Studies of avian Rous sarcoma virus (RSV) led to the discovery of the viral oncogene src, and this was followed by the discovery of other viral oncogenes in retroviruses of mammals including rodents, cats, monkeys and so forth. Studies of the viral oncogenes in turn led to the discovery of cellular proto-oncogenes in the host genome; cellular oncogenes have been shown to be activated in a variety of human cancers, including those with no viral involvement. Oncogenic animal retroviruses can be divided into two groups based on their mechanisms of tumourigenesis, acute transforming retroviruses and nonacute retroviruses. Acute transforming retroviruses are typically replication defective and they induce tumours rapidly due to expression of their viral oncogenes. Nonacute retroviruses are replication competent and they induce tumours with longer latencies, by activating cellular proto-oncogenes in the tumour cells; this results from insertion of proviral DNA in the vicinity of the activated proto-oncogene. More recently, human T-cell leukaemia virus type I (HTLV-I) was discovered as an etiological agent of human cancer (adult T-cell leukaemia [ATL]); this virus also encodes regulatory genes some of which are important for its oncogenic potential. Most recently, the retroviral structural protein Envelope (Env) has been shown to be directly involved in oncogenic transformation for certain retroviruses. Env-induced transformation is a new paradigm for retroviral oncogenesis. In this review, we will summarise research on retrovirus oncogenic transformation over the past 100 years since the first published report of an oncogenic virus with particular attention to Env-induced transformation.

show abstract

Molecular Cloning and Functional Analysis of Three Type D Endogenous Retroviruses of Sheep Reveal a Different Cell Tropism from That of the Highly Related Exogenous Jaagsiekte Sheep Retrovirus

Cited by 106 publications

References 66 publications

Evolutionary dynamics of endogenous Jaagsiekte sheep retroviruses proliferation in the domestic sheep, mouflon and Pyrenean chamois

Evolutionary dynamics of endogenous Jaagsiekte sheep retroviruses proliferation in the domestic sheep, mouflon and Pyrenean chamois

Jaagsiekte Sheep Retrovirus and Enzootic Nasal Tumor Virus Promoters Drive Gene Expression in All Airway Epithelial Cells of Mice but Only Induce Tumors in the Alveolar Region of the Lungs

Oncogenesis by retroviruses: old and new paradigms

Contact Info

Product

Resources

About