Molecular cloning and expression study on Toll-like receptor 5 paralogs in Japanese flounder, Paralichthys olivaceus

Cloning and characterization of the grass carp (Ctenopharyngodon idella) Toll-like receptor 22 gene, a fish-specific gene

“…In other words, when comparing freshwater (D. rerio, C. idella and O. mykiss) and seawater teleost species (T. rubripes, P. olivaceus and P. crocea) the latter tended to have more introns in TLR22 gene. Similar results were found for TLR2 [36], TLR5 [37] and TLR9 [38] in fish species and this warrants further investigation. Diversity in TLR gene organization was also reported for TLR1, TLR2, TLR3 and TLR5 within and between teleosts as well as in mammals [22,36,37,39], but it is as yet unclear whether this diversity causes functional differences.…”

Maximal transcriptional activation of piscine soluble Toll-like receptor 5 by the NF-κB subunit p65 and flagellin

“…The complete ORF obtained by joining sequences of the EIFL-2-C4 and 5 0 -and 3 0 -RACE PCR products gave a predicted TLR5S precursor of 641 amino acids (AAs), which included a 31-AA signal peptide (GenBank accession no. JF266562), in accordance with Hwang et al [19].…”

“…TLR5S consists of two exons (GenBank accession no. JF266563), in accordance with Hwang et al [19]. The promoter region of TLR5S was further sequenced from each BAC clone by primer walking.…”

“…More recently, membrane and soluble TLR5 paralogs were reported in the Japanese flounder Paralichthys olivaceus [19]. These paralogs, TLR5M and TLR5S, respectively, exist individually and show different expression patterns against the bacterial pathogen Edwardsiella tarda.…”

Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection

“…In teleost fish, two types of TLR5 were existed: the membrane form of TLR5 (TLR5M) and the soluble form of TLR5 (TLR5S). Until now, TLR5S was cloned from rainbow trout (Onchorhynchus mikiss) [14], atlantic salmon (Salmo salar) [15], catfish (Ictalurus punctatus) [16], Japanese flounder (Paralichthys olivaceus) [17], and gilthead seabream (Sparus aurata) [18]. However, in zebrafish (Danio rerio), common carp (Cyprinus carpio), and Indian major carp (Cirrhinus mrigala), only TLR5M was found [19,20].…”