Molecular cloning and expression profile of an abiotic stress and hormone responsive MYB transcription factor gene from <italic>Panax ginseng</italic>

Abstract

The v-myb avian myeloblastosis viral oncogene homolog (MYB) family constitutes one of the most abundant groups of transcription factors and plays vital roles in developmental processes and defense responses in plants. A ginseng (Panax ginseng C.A. Meyer) MYB gene was cloned and designated as PgMYB1. The cDNA of PgMYB1 is 762 base pairs long and encodes the R2R3-type protein consisting 238 amino acids. Subcellular localization showed that PgMYB1-mGFP5 fusion protein was specifically localized in the nucleus. To understand the functional roles of PgMYB1, we investigated the expression patterns of PgMYB1 in different tissues and under various conditions. Quantitative real-time polymerase chain reaction and western blot analysis showed that PgMYB1 was expressed at higher level in roots, leaves, and lateral roots than in stems and seeds. The expression of PgMYB1 was up-regulated by abscisic acid, salicylic acid, NaCl, and cold (chilling), and downregulated by methyl jasmonate. These results suggest that PgMYB1 might be involved in responding to environmental stresses and hormones.

Abscisic Acid Regulates the 3-Hydroxy-3-methylglutaryl CoA Reductase Gene Promoter and Ginsenoside Production in Panax quinquefolium Hairy Root Cultures

“…It has been noted that increased activation of some genes and the biosynthesis of specific proteins may improve the adaptation of the plant to drought or salt-stress conditions. For example, in Panax sp., the expression of S-adenosyl-L-methionine synthetase, PgWRKY1,2,4,5,8,9, LHT-type plant amino acid-transporter, and MYB transcription factor is activated in response to ABA treatment [16,17,18,19,20]. Literature data also indicates that abscisic acid can regulate the biosynthesis of secondary metabolites.…”

Analysis of Panax ginseng miRNAs and Their Target Prediction Based on High-Throughput Sequencing

“…Members of the MYB transcription factor family are known to play roles in plant development and secondary metabolism [14]. MYB families have been reported to regulate the accumulation of saponins in P. ginseng [15]. miR164 targets NAC (NAM/ATAF/CUC) transcription factors (NAC), which play a role in the tolerance of dehydration by regulating the genes related to osmotic stress in rose petals [16] and affecting the height of rice by mediating the gibberellic pathway [17].…”

Isoform Sequencing Provides a More Comprehensive View of the Panax ginseng Transcriptome

“…As represented in Figure 4 , a comparative analysis of transcription factor gene structure showed that when compared to Arabidopsis and rice, P. ginseng contained a higher proportion of transcription factors that control secondary metabolism, cell morphogenesis, and transmission of hormone and stress signals, including MYB, WRKY, C3H, B3, and HB. MYB and WRKY are involved within the synthesis of the secondary metabolites of ginseng [ 44 , 45 ]. C3H, B3, and HB are responsible for precisely controlling gene expression in Arabidopsis and rice following environmental changes [ 46 , 47 ].…”