Molecular cloning and expression of bovine (Bos taurus) leptin receptor isoform mRNAs

Kawachi, Hiroyuki; Yang, Seung-Hak; Hamano, Ai; Matsui, Tohru; Smith, Stephen B.; Yano, Hideo

doi:10.1016/j.cbpb.2007.05.007

Cited by 16 publications

(13 citation statements)

References 36 publications

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“…In sheep, hypothalamic Lepr-b expression is increased during fasting, food restriction, and lactation (1,16,29,39). In prepubertal dairy heifers, we showed that Lepr-b is the most abundant isoform expressed in the hypothalamus, whereas Lepr-a accounted for nearly all of Lepr transcripts in peripheral tissues (41), consistent with the spatial distribution of Lepr isoforms reported by others (27). We also found that estrogen had no effect on peripheral Lepr expression, except in the uterus and mammary adipose tissue, where it reduced Lepr-a and Lepr-b transcript abundance (41).…”

supporting

confidence: 77%

Insulin regulates hepatic leptin receptor expression in early lactating dairy cows

Thorn¹,

Ehrhardt²,

Butler³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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Energy balance controls the expression of the leptin receptor (Lepr) in the ruminant hypothalamus but whether similar regulation occurs in peripheral tissues is unknown. To address this issue, we measured Lepr expression in the liver and adipose tissue of dairy cows during the transition from late pregnancy (LP) to early lactation (EL). This period is characterized by the development of a profound state of energy insufficiency and is associated with reduced plasma insulin and leptin and with increased plasma growth hormone. Hepatic expression of the short (Lepr-a) and long (Lepr-b) isoforms was 40% higher during EL (8 days postpartum) than LP (30 days prepartum). A similar effect was observed when negative energy balance was induced in nonpregnant, late-lactation dairy cows by food restriction, implicating energy insufficiency as a specific cause in EL. The stimulation of hepatic Lepr expression was reversed after a 48-h period of hyperinsulinemic euglycemia in EL. Changes in hepatic Lepr expression during chronic elevation of plasma leptin in EL or plasma growth hormone in nonpregnant, late-lactation cows did not support a role for these hormones in mediating the effects of energy insufficiency on hepatic Lepr expression. In adipose tissue, Lepr expression was increased 10-fold during the transition from LP to EL. Overall, these data indicate that hypoinsulinemia is partly responsible for the induction of Lepr expression in the liver, and perhaps adipose tissue, of energy-deficient dairy cows.

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supporting

confidence: 77%

Insulin regulates hepatic leptin receptor expression in early lactating dairy cows

Thorn¹,

Ehrhardt²,

Butler³

et al. 2008

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Actually, ObRb, ObRa and ObRc are all strongly expressed in bovine intermuscular, renal and s.c. fat (Kawachi et al, 2007) suggesting that these tissues respond to leptin in addition to secreting it while ObRa was found also in lactating ewe mammary gland (Laud et al, 1999). Ogasawara et al (2008) identified ObRb in the anterior pituitaries of Holstein bull calves, with a similar distribution of somatotrophs (60%) and gonadotrophs (16%) to that seen in mature ewes (Iqbal et al, 2000).…”

Section: Wylie 248mentioning

confidence: 93%

Leptin in farm animals: where are we and where can we go?

Wylie

2011

Animal

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Fat affects meat quality, value and production efficiency as well as providing energy reserves for pregnancy and lactation in farm livestock. Leptin, the adipocyte product of the obese (ob) gene, was quickly seen as a predictor of body fat content in animals approaching slaughter and an aid to assessing reproductive readiness in females. Its participation in inflammation and immune responses that help animals survive infection and trauma has clear additional relevance to meat and milk production. Furthermore, almost a decade of discoveries of nucleotide polymorphisms in the leptin and leptin receptor genes has suggested useful applications relating to feed intake regulation, the efficiency of feed use, the composition of growth, the timing of puberty, mammogenesis and mammary gland function and fertility in cattle, pigs and poultry. The current review attempts to summarise where research has taken us in each of these aspects and speculates on where future research might lead.Keywords: leptin, farm animals, production efficiency, fat, body composition ImplicationsLeptin has not yet delivered the initial expectations of animal scientists eager for a robust and reliable marker of body and carcase fat in farm livestock, but its close association with fat and fat deposition continue to cast it as a potential aid for enhancing production efficiency, reproductive success and immune function. Polymorphisms in the leptin and leptin receptor genes provide powerful additional tools for enhanced selection to meet aspirations for increased global animal production within an overarching need to contain and reduce environmental impacts. IntroductionFat is important in all livestock production as it affects product quality and production efficiency and provides the energy reserves for pregnancy and lactation. The discovery of the obese (ob) gene and its product leptin (Zhang et al., 1994;Halaas et al., 1995) and their links to body fat (Frederich et al., 1995;Maffei et al., 1995) excited animal scientists, who quickly saw potential for leptin as a predictor of body composition in animals approaching slaughter and of reproductive readiness in females (Hossner, 1998). Its involvement in inflammation and in the immune responses that help animals survive infection and trauma, also has clear relevance and attention turned to using leptin, directly or indirectly, to influence production characteristics with feed intake, body composition, puberty, mammary gland function, immune response and the reproductive pathway all possible targets. Associations of production traits with polymorphisms in the leptin and leptin receptor genes pointed to additional applications.Among the many reviews of leptin, those by ), Baile et al. (2000, Ingvartsen and Boisclair (2001), Chilliard et al. (2005) and Barb et al. (2006) stand out as most relevant to livestock with Williams et al. (2002), Barb and Kraeling (2004), Barb et al. (2005) and Zieba et al. (2005) emphasising its involvement in reproduction. Those by Ahima and Flier (2000) and Harris (200...

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“…Six different receptor isoforms has been detected for leptin. Only the long form (Ob-Rb) is functional and is responsible for the leptin physiological effects (Tartaglia 1997;Kawachi et al 2007). The tissue distribution of leptin receptor is recently identified in cattle.…”

Section: Introductionmentioning

confidence: 99%

Leptin mRNA expresses in the bull reproductive organ

et al. 2009

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Leptin, a 167-amino acid hormone, is secreted mainly by fat tissue. It has some powerful effects on the regulation of metabolism and reproductive function through endocrine and probably paracrine mechanisms. The contribution rate of leptin function on the male reproductive system is not still clear. Characterization of leptin expression in reproductive organs will suggest that in addition to its endocrine action, leptin has also paracrine/autocrine effects on reproduction. The expression of functional leptin receptor mRNA has been already recognized in testis of rodents, human and cattle. Thus, the aim of the present study was to investigate the presence of leptin mRNA in the bovine testis, because it will be the first step for understanding of its paracrine/autocrine effects on the male reproductive organs in cattle. The present study was the first to showed leptin mRNA expression in the testis of Holstein cattle using reverse transcription and polymerase chain reaction (RT-PCR) analysis. RT-PCR products were amplified with nested PCR using inner leptin primer pairs to emphasis the first results. Besides, bovine beta actin gene was acted as an internal positive control as well as RNA purification marker. Our findings suggest that in addition to its endocrine actions at the hypothalamic-pituitary axis, leptin can has an autocrine and/or paracrine role in bull testicular function.

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Molecular cloning and expression of bovine (Bos taurus) leptin receptor isoform mRNAs

Cited by 16 publications

References 36 publications

Insulin regulates hepatic leptin receptor expression in early lactating dairy cows

Insulin regulates hepatic leptin receptor expression in early lactating dairy cows

Leptin in farm animals: where are we and where can we go?

Leptin mRNA expresses in the bull reproductive organ

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