Molecular Cloning and Expression of Phytoene Synthase, Lycopene Beta-cyclase, and Beta-carotene Hydroxylase Genes in Persimmon (Diospyros kaki L.) Fruits

Zhao, Daqiu; Zhou, Chunhua; Sheng, Yanle; Liang, Guohua; Tao, Jun

doi:10.1007/s11105-010-0238-5

Cited by 38 publications

(19 citation statements)

References 26 publications

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“…Therefore, high carotenoids content, not only play an important role in biological property, but also are favored from the marketing perspective (Nakagawa et al 2008;Robert et al 2010). In recent years, with the clarification of carotenoids biosynthesis pathway in some model plants ( Bartley and Scolnik 1995;Tao et al 2002;Galpaz et al 2006), the researches on carotenoids accumulation regulation of other plants are gradually deepening, and many carotenogenic have been cloned and manipulated (Ma et al 2010;Rodríguez-Ávila et al 2010;Sun et al 2010;Zhao et al 2010) (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Carotenoid Accumulation and Carotenogenic Genes Expression During Two Types of Persimmon Fruit (Diospyros kaki L.) Development

2010

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Persimmon (Diospyros kaki L.), belonging to the Ebenaceae family, has been used not only as a fresh fruit, but also for many medicinal uses. Carotenoids are the main pigment in persimmon fruit, which contribute significantly to fruit color and nutritional quality due to their composition and content. In this study, fruit quality indices, carotenoid contents and expression of carotenogenic genes were analyzed in two types of persimmon fruit. The results demonstrated that there was a positive correlation between fruit color and the contents of main composition and total carotenoids. Carotenoid accumulation in persimmon fruit resulted from the interaction of carotenogenic genes, but the molecular mechanisms responsible for accumulation of carotenoids in two types of persimmon fruit had a few differences. As a complete unit, the relatively low expression level of phytoene synthase gene (DkPSY) in "Niuxinshi" resulted in low carotenoid contents or even under the detection limit at the early fruit developmental stages; but low carotenoid contents in "Nishimurawase" were due to the relatively low expression level of carotenogenic genes other than DkPSY. At the late fruit developmental stages, increased expression levels of DkPSY, phytoene desaturase gene and beta-carotene hydroxylase gene (DkBCH) induced elevated carotenoid contents; because all carotenogenic genes strongly expressed in "Nishimurawase", a large amount of carotenoids were accumulated. In addition, β-cryptoxanthin was the main composition whose content increased with the fruit maturity changes, which was mainly because of DkBCH which might lead more conversion of β-carotene to β-cryptoxanthin.

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Section: Introductionmentioning

confidence: 99%

Carotenoid Accumulation and Carotenogenic Genes Expression During Two Types of Persimmon Fruit (Diospyros kaki L.) Development

2010

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“…Persimmon is not only an important fruit, which is eaten as a fresh fruit, but also traditionally used for many medicinal purposes [11].…”

Section: Discussion:-mentioning

confidence: 99%

Analysis of Main Quality Characteristics of Persimmon (Diospyros Kaki L.) Fruits.

MohamedElHadi¹,

Bu²,

Ge³

et al. 2017

IJAR

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Persimmon (Diospyros kaki L.) is an important commercial and deciduous fruit tree with lots of cultivars. It is grown in various countries and is increasingly appreciated for its nutritional value, health benefits and rich flavor. In this research, persimmon fruits were used as plant materials to evaluate the main fruit quality characteristics of 34 different cultivars and to study the effect of ethylene and salicylic acid (SA) in main quality characteristic of two persimmon cultivars, namely "Xiaofangshi" and "Naiyoushi", during different ripening stages. The average weight of fruit of non-astringent persimmon cultivars was heavier compared to astringent persimmon, while the differences in fruit shapes index among all the astringent persimmon cultivars was larger than that of non-astringent persimmon cultivars. Moreover, the average total soluble solids of the astringent persimmon cultivars was higher than that of the non-astringent persimmons, while titratable acidity was basically consistent in both non-astringent and astringent persimmon cultivars. On the other hand, results showed that there were no significant differences in main fruit quality including fruit weight, diameter and fruit shape index in two different cultivars during different ripening stages. While, both fruit firmness and total soluble solids showed significant differences in both two cultivars, results revealed that fruit treated with ethylene had the lowest firmness and the highest total soluble solids followed by control fruits, while fruit treated with SA had the highest firmness and the lowest total soluble solids. A comparison of fruit color between different persimmon cultivars showed that both the pulp and the peel of astringent persimmon had deeper orange red color compared to non-astrignant cultivar. Moreover, hue angle (H°) of both "Xiaofangshi" and "Naiyoushi" was higher at harvest then decreased in different treatment during different ripening stages reflected the development in the color of fruit to deep orange.Copy Right, IJAR, 2017,. All rights reserved.

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“…SWISS-MODEL (http://swissmodel.expasy. org/) was used to analyze the molecular modeling of MmRPS3a protein (Zhao et al, 2010).…”

Section: Bioinformatics Analysis Of the Mmrps3a Genementioning

confidence: 99%

Molecular cloning and expression analyses of RPS3a gene from mulberry under abiotic stresses and among different mulberry varieties

et al. 2016

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ABSTRACT.A full-length cDNA sequence coding ribosomal protein S3a of mulberry tree, which we designated MmRPS3a (GenBank accession No. KR610331), was cloned based on mulberry expressed sequence tags. Sequence analysis showed that the MmRPS3a is 1089 bp long and contains a 80-bp 5'-UTR (untranslated region) and a 220-bp 3'-UTR. Its open reading frame consists of a 789-bp encoding 262 amino acids with a predicted molecular weight of 30.053 kDa and an isoelectric point of 9.84. Homology analysis revealed that MmRPS3a gene is highly conservative in mulberry and other species including Morus notabilis, Theobroma cacao, and Ricinus communis. Phylogenetic analysis based on MmRPS3a of other species showed that mulberry had a closer relationship with Prunus persica, Arabidopsis The results of quantitative PCR analysis showed that the transcriptional level of MmRPS3a mRNA changed significantly under the conditions of hypothermia, aridity, salt stress, and varieties of differing resistances.

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Molecular Cloning and Expression of Phytoene Synthase, Lycopene Beta-cyclase, and Beta-carotene Hydroxylase Genes in Persimmon (Diospyros kaki L.) Fruits

Cited by 38 publications

References 26 publications

Carotenoid Accumulation and Carotenogenic Genes Expression During Two Types of Persimmon Fruit (Diospyros kaki L.) Development

Carotenoid Accumulation and Carotenogenic Genes Expression During Two Types of Persimmon Fruit (Diospyros kaki L.) Development

Analysis of Main Quality Characteristics of Persimmon (Diospyros Kaki L.) Fruits.

Molecular cloning and expression analyses of RPS3a gene from mulberry under abiotic stresses and among different mulberry varieties

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