Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype.

Shen, Yong; Monsma, Frederick J.; Metcalf, Mark A.; José, Pedro A.; Hamblin, Mark W.; Sibley, David R.

doi:10.1016/s0021-9258(17)46830-x

Cited by 472 publications

(115 citation statements)

References 29 publications

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“…33 Other studies have shown that AC is stimulated by a 5-HT 7 receptor in mammalian cells. 20,[34][35][36][37][38][39][40] In this study, the expression of DDX3 under the effect of PKA inhibitor after treating HepG2 cells with AS-19 and cAMP confirmed that PKA could block the effect of AS-19 and cAMP on DDX3 promoter expression (Figure 6A and 6C). Likewise, adenylyl cyclase playing a role as a regulatory factor via specific cAMP-binding proteins must be involved through interaction with AS-19-and 5-HT-mediated cAMP pathways (Figure 6B and 6D).…”

Section: Discussionmentioning

confidence: 53%

Stimulating DDX3 expression by serotonin 5‐HT receptor 7 through phosphorylation of p53 via the AC‐PKA‐ERK signaling pathway

Kang

Nguyen

Eom

et al. 2019

J of Cellular Biochemistry

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DDX3 is a host viral factor that can inhibit the hepatitis B virus‐induced innate immune responses. In this study, the 20 bioactive compounds have screened the effects on DDX3 and we found that 5‐HT upregulated DDX3 promoter activity via the 5‐HT7 receptor on liver hepatocellular cells (HepG2 cells) by using a luciferase assay, reverse transcription‐polymerase chain reaction analysis, and Western blot analysis. Furthermore, we are trying to elucidate the pathways involved in the stimulating effect of 5‐HT on DDX3 expression to induce innate immune responses against hepatitis B virus infection. A knockdown of the 5‐HT7 receptor by transfection si‐5‐HT7 receptors or si‐control into HepG2 cells treated by 5‐HT (or 5‐HT plus agonist) confirmed the role of the 5‐HT7 receptor in DDX3 expression. The IFN‐β‐Luc expression and level of hepatitis B virus surface Antigen (HBsAg) showed that DDX3 mediated by the 5‐HT7 agonist (AS‐19) increased IFN‐β expression and inhibited HBV replication. Luciferase assays showed the involvement of 5‐HT7 receptors in DDX3 expression via cAMP/AC/PKA pathways by using protein kinase A (PKA) and adenylyl cyclase inhibitor (MDL 12330A). AS‐19 mediated DDX3 promoter activated PKA extracellular signal‐regulated kinase ERK signaling the p53 phosphorylation (‐1080/‐1070) resulted in upregulation of DDX3 promoter transactivation via the 5‐HT7 receptors agonist. Overall, 5‐HT7 was found to be a new potential target to inhibit hepatitis B infection by activating AC/PKA/ERK pathways by phosphorylating p53 via the 5‐HT7 agonist response by mediating DDX3 expression.

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Section: Discussionmentioning

confidence: 53%

Stimulating DDX3 expression by serotonin 5‐HT receptor 7 through phosphorylation of p53 via the AC‐PKA‐ERK signaling pathway

Kang

Nguyen

Eom

et al. 2019

J of Cellular Biochemistry

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“…SB258719, a 5-HT 7 antagonist ( 56 ), produced a reduction in the contractile response of the BTSM induced by 5-HT. These receptors are associated mainly with a G s protein capable of activating adenylate cyclase (AC) and thereby produced an increase in the generation of the cyclic adenosine monophosphate (cAMP) inside cells of the ASM ( 57 , 58 ), which would cause relaxation of the muscle ( 59 , 60 , 61 ). Nevertheless, recent studies established that these receptors can activate another less known signalling pathway mediated by G 12 protein ( 57 , 62 ).…”

Section: Discussionmentioning

confidence: 99%

5-HT2A, 5-HT1B/D, 5HT3 and 5-HT7 receptors as mediators of serotonin-induced direct contractile response of bovine airway smooth muscle

Guevara

Trejo

2021

J. Smooth Muscle Res.

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Background: Serotonin (5-hydroxytryptamine; 5-HT) performs a variety of functions in the body including the modulation of muscle tone in respiratory airways. Several studies indicate a possible role of 5-HT in the pathophysiology of bronchial hyperresponsiveness. However, the receptors and the molecular mechanisms by which 5-HT acts on airway smooth muscle (ASM) continue to be controversial. Most of the evidence suggests the participation of different subtypes of receptors in an indirect response. This study supports the proposal that 5-HT directly contracts ASM and characterizes pharmacologically the subtypes of serotonergic receptors involved. The characterization was carried out by using selective antagonists in an organ bath model allowing study of the smooth muscle of segments of bovine trachea. Results: The results obtained show that 5-HT 2A receptors are the main mediators of the direct contractile response of bovine ASM, with the cooperation of the 5-HT 7 , 5-HT 3 and 5-HT 1B/D receptors. Also, it was observed that the muscle response to serotonin is developed more slowly and to a lesser extent in comparison with the response to cholinergic stimulation. Conclusion: Overall, the receptors that mediate the direct serotonergic contraction of the smooth muscle of the bovine trachea are 5-HT 2A , 5-HT 7 , 5-HT 3 and 5-HT 1B/D receptors.

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“…The 5-HT7 receptors are involved in the sleep-wake cycle, body temperature, depression-like behavior and the processes of learning and memory ( 240 – 242 ). In the brain, they are mainly expressed in the thalamus, hypothalamus, hippocampus, prefrontal cortex, amygdala, lateral habenula, raphe nuclei, and the suprachiasmatic nucleus ( 240 , 243 , 244 ). 5-HT7 receptors couple to Gαs ( 245 ) and Gα12 ( 206 ).…”

Section: Axonal and Neurite Outgrowth: Serotonin Receptorsmentioning

confidence: 99%

Rewiring of the Serotonin System in Major Depression

Vahid-Ansari

Albert

2021

Front. Psychiatry

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Serotonin is a key neurotransmitter that is implicated in a wide variety of behavioral and cognitive phenotypes. Originating in the raphe nuclei, 5-HT neurons project widely to innervate many brain regions implicated in the functions. During the development of the brain, as serotonin axons project and innervate brain regions, there is evidence that 5-HT plays key roles in wiring the developing brain, both by modulating 5-HT innervation and by influencing synaptic organization within corticolimbic structures. These actions are mediated by 14 different 5-HT receptors, with region- and cell-specific patterns of expression. More recently, the role of the 5-HT system in synaptic re-organization during adulthood has been suggested. The 5-HT neurons have the unusual capacity to regrow and reinnervate brain regions following insults such as brain injury, chronic stress, or altered development that result in disconnection of the 5-HT system and often cause depression, anxiety, and cognitive impairment. Chronic treatment with antidepressants that amplify 5-HT action, such as selective serotonin reuptake inhibitors (SSRIs), appears to accelerate the rewiring of the 5-HT system by mechanisms that may be critical to the behavioral and cognitive improvements induced in these models. In this review, we survey the possible 5-HT receptor mechanisms that could mediate 5-HT rewiring and assess the evidence that 5-HT-mediated brain rewiring is impacting recovery from mental illness. By amplifying 5-HT-induced rewiring processes using SSRIs and selective 5-HT agonists, more rapid and effective treatments for injury-induced mental illness or cognitive impairment may be achieved.

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Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype.

Cited by 472 publications

References 29 publications

Stimulating DDX3 expression by serotonin 5‐HT receptor 7 through phosphorylation of p53 via the AC‐PKA‐ERK signaling pathway

Stimulating DDX3 expression by serotonin 5‐HT receptor 7 through phosphorylation of p53 via the AC‐PKA‐ERK signaling pathway

5-HT<sub>2A</sub>, 5-HT<sub>1B/D</sub>, 5HT<sub>3</sub> and 5-HT<sub>7</sub> receptors as mediators of serotonin-induced direct contractile response of bovine airway smooth muscle

Rewiring of the Serotonin System in Major Depression

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