Molecular cloning and expression of the Caenorhabditis elegans klp-3, an ortholog of C terminus motor kinesins kar3 and ncd

Khan, Muhummadh; Gogonea, Camelia B; Siddiqui, Zeba K.; Ali, M. Yusuf; Kikuno, Reiko; Nishikawa, Ken; Siddiqui, Shahid

doi:10.1006/jmbi.1997.1112

Cited by 17 publications

(18 citation statements)

References 44 publications

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“…A C-terminal motor subfamily member klp-3 is proposed to be involved in chromosome separation, as overexpression of klp-3 reduces the incidence of males caused by nondisjunction of the X-chromosome, and depletion of klp-3 transcript results in embryonic lethality and the production of polyploid cells. However, the subcellular distribution of KLP-3 and microtubule organization in KLP-3-depleted embryos have yet to be reported (Khan et al, 1997).…”

Section: Zen-4 Organizes the Midzone Microtubulesmentioning

confidence: 99%

Cytokinesis and Midzone Microtubule Organization inCaenorhabditis elegansRequire the Kinesin-like Protein ZEN-4

Raich¹,

Moran

Rothman

et al. 1998

MBoC

261

254

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Members of the MKLP1 subfamily of kinesin motor proteins localize to the equatorial region of the spindle midzone and are capable of bundling antiparallel microtubules in vitro. Despite these intriguing characteristics, it is unclear what role these kinesins play in dividing cells, particularly within the context of a developing embryo. Here, we report the identification of a null allele ofzen-4, an MKLP1 homologue in the nematodeCaenorhabditis elegans, and demonstrate that ZEN-4 is essential for cytokinesis. Embryos deprived of ZEN-4 form multinucleate single-celled embryos as they continue to cycle through mitosis but fail to complete cell division. Initiation of the cytokinetic furrow occurs at the normal time and place, but furrow propagation halts prematurely. Time-lapse recordings and microtubule staining reveal that the cytokinesis defect is preceded by the dissociation of the midzone microtubules. We show that ZEN-4 protein localizes to the spindle midzone during anaphase and persists at the midbody region throughout cytokinesis. We propose that ZEN-4 directly cross-links the midzone microtubules and suggest that these microtubules are required for the completion of cytokinesis.

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Section: Zen-4 Organizes the Midzone Microtubulesmentioning

confidence: 99%

Cytokinesis and Midzone Microtubule Organization inCaenorhabditis elegansRequire the Kinesin-like Protein ZEN-4

Raich¹,

Moran

Rothman

et al. 1998

MBoC

261

254

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“…28 At the molecular level, the complete C. elegans genome sequence has made possible organism-wide identification and comparison of predicted proteins. C. elegans has classical 29 and peptidergic 30,31 neurotransmitters, their associated receptors, neural-specific cytoskeletal proteins, [32][33][34] signal transduction molecules, 35 ligand or voltage-gated ion channels, and chemosensory G-protein-coupled receptors. 36 Given the conservation at the molecular level, drugs with neurological targets in vertebrates often have profound effects on C. elegans behavior.…”

Section: Analysis Of Neuropeptides In C Elegansmentioning

confidence: 99%

The ever-expanding neuropeptide gene families in the nematode Caenorhabditis elegans

2006

Parasitology

115

162

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Neuropeptides act as chemical signals in the nervous system to modulate behaviour. With the ongoing EST projects and DNA sequence determination of different genomes, the identification of neuropeptide genes has been made easier. Despite the relatively 'simple' repertoire of behaviours in the nematode Caenorhabditis elegans, this worm contains a surprisingly large and diverse set of neuropeptide genes. At least 109 genes encoding over 250 potential neuropeptides have been identified in C. elegans; all genes are likely to be expressed and many, if not all, of the predicted peptides are produced. The predicted peptides include: 38 insulin-like peptides, several of which are involved in development and reproductive growth, and over 70 FMRFamide-related peptides, some of which are involved in locomotion, reproduction, and social behaviour. Many of the C. elegans peptides are identical or highly similar to those isolated or predicted in parasitic nematodes, such as Ascaris suum, Haemonchus contortus, Ancylostoma caninum, Heterodera glycines and Meloidogyne arenaria, suggesting that the function of these peptides is similar across species. The challenge for the future is to determine the function of all the genes and individual peptides and to identify the receptors through which the peptides signal.

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“…Thus, we may ask how many different kinesins participate in a given cellular process, and whether they perform a unique or redundant function. We previously identified 20 genes encoding k inesin‐ l ike p roteins ( klp s) in C. elegans [6]; four of these kinesins, KLP‐3, KLP‐15, KLP‐16 and KLP‐17, contain C‐terminal motors, similar to the Drosophila NCD and the yeast KAR3 kinesin. Members of the NCD subfamily of kinesin are known to attach with spindles and facilitate chromosome distribution to different poles during mitotic division [1–4].…”

Section: Introductionmentioning

confidence: 99%

“…Members of the NCD subfamily of kinesin are known to attach with spindles and facilitate chromosome distribution to different poles during mitotic division [1–4]. Similarly, C. elegans KLP‐3 and KLP‐17 are also involved in chromosomal movement and segregation [6,7].…”

Section: Introductionmentioning

confidence: 99%

A novel C‐terminal kinesin subfamily may be involved in chromosomal movement in Caenorhabditis elegans¹

et al. 2000

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C-terminal kinesin motor proteins, such as the Drosophila NCD and yeast KAR3, are involved in chromosomal segregation. Previously we have described two orthologs of NCD in Caenorhabditis elegans, KLP-3 and KLP-17, which also participate in chromosome movement. Here we report cDNA cloning of klp-15 and klp-16, and the expression pattern of the genes encoding C-terminal motor kinesins including klp-15 and klp-16. Interestingly KLP-15 and KLP-16 form a unique class of C-terminal kinesins, distinct from the previously known C-terminal motors in other organisms. Using in situ hybridization and RNA interference assay, we show that although all of these motors mediate chromosome segregation, they do so in a combination of unique and overlapping manners, suggesting a complex hierarchy of kinesin motor function in metazoans.z 2000 Federation of European Biochemical Societies.

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Molecular cloning and expression of the Caenorhabditis elegans klp-3, an ortholog of C terminus motor kinesins kar3 and ncd

Cited by 17 publications

References 44 publications

Cytokinesis and Midzone Microtubule Organization inCaenorhabditis elegansRequire the Kinesin-like Protein ZEN-4

Cytokinesis and Midzone Microtubule Organization inCaenorhabditis elegansRequire the Kinesin-like Protein ZEN-4

The ever-expanding neuropeptide gene families in the nematode Caenorhabditis elegans

A novel C‐terminal kinesin subfamily may be involved in chromosomal movement in Caenorhabditis elegans¹

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Molecular cloning and expression of the Caenorhabditis elegans klp-3, an ortholog of C terminus motor kinesins kar3 and ncd

Cited by 17 publications

References 44 publications

Cytokinesis and Midzone Microtubule Organization inCaenorhabditis elegansRequire the Kinesin-like Protein ZEN-4

Cytokinesis and Midzone Microtubule Organization inCaenorhabditis elegansRequire the Kinesin-like Protein ZEN-4

The ever-expanding neuropeptide gene families in the nematode Caenorhabditis elegans

A novel C‐terminal kinesin subfamily may be involved in chromosomal movement in Caenorhabditis elegans1

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A novel C‐terminal kinesin subfamily may be involved in chromosomal movement in Caenorhabditis elegans¹