Molecular cloning and expression analysis of a RanBP2 zinc finger protein gene in upland cotton (Gossypium hirsutum L.)

Construction of cDNA library of cotton mutant (Xiangmian-18) library during gland forming stage

“…We constructed cDNA library during pigment gland formation stage, the ultimate goal of which was to collect some full-length cDNA related to gland development [24]. The unique DSN normalization technology [25] and SMART technique would overcome the problem caused by different expression frequencies of genes and it was an efficient tool for gene identification [26,27].…”

“…1). So the stage from 38 to 52 h was considered to be the developing gland stage [24]. Seeds were selected randomly from 38 to 52 h to extract mRNA.…”

Fine mapping of the dominant glandless Gene Gl 2 e in Sea-island cotton (Gossypium barbadense L.)

“…However, the upland cotton cultivars have never been developed. At present, researchers have cloned a number of genes related to gossypol biosynthesis [22][23][24][25][26][27][28] and further provided useful information for the mechanism of gossypol biosynthesis. Recently, Sunilkumar et al [29] have successfully used RNAi in combination with seed special promoter to disrupt gossypol biosynthesis in cottonseed tissue by interfering with the expression of the δ-cadinene synthase gene (CAD1) during seed development.…”

Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton

“…In the past, the research was mainly focused on the biosynthesis of gossypol (Townsend et al 2005), as the storage organ of gossypol and other TAs, the pigments gland began to attract the attention of some researchers (Zhu et al 1999;Chang et al 2007), but the regulatory mechanism between the storage organ gland and its inclusions TAs including gossypol in cotton has not been disclosed up to the present.…”