Molecular cloning and expression analysis of mulberry MAPK gene family

Wei, Congjin; Liu, Xueqin; Long, Dan; Guo, Qing; Fang, Yuan; Bian, Chenkai; Zhang, Dayan; Zeng, Qiwei; Zhang, Xiang; Zhao, Aichun

doi:10.1016/j.plaphy.2014.02.002

Cited by 48 publications

(40 citation statements)

References 40 publications

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“…We believed CaDHN4 might has an important role in cold condition. Many studies have indicated that plant hormone (ABA, SA and MeJA) play a key role in adaptive environmental stresses and plant developmental processes [49,50]. Dehydrins proteins have an important role in defenses against stress, in either an ABA-dependent or ABA–independent pathway.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification, Expression Diversication of Dehydrin Gene Family and Characterization of CaDHN3 in Pepper (Capsicum annuum L.)

Hua

et al. 2016

PLoS ONE

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Dehydrins (DHNs) play a crucial role in enhancing abiotic stress tolerance in plants. Although DHNs have been identified and characterized in many plants, there is little known about Capsicum annuum L., one of the economically important vegetable crops. In this study, seven CaDHNs in the pepper genome were identified, which could be divided into two classes: YnSKn- and SKn-type, based on their highly conserved domains. Quantitative real-time PCR (qRT-PCR) results showed that the seven DHN genes were expressed in all tissues and might be involved in the growth and development of pepper. The gene expression profiles analysis suggested that most of the CaDHN genes were induced by various stresses (low temperature, salt and mannitol) and signaling molecules (ABA, SA and MeJA). Furthermore, the CaDHN3 (YSK2)-silenced pepper plants showed obvious lower resistance to abiotic stresses (cold, salt and mannitol) than the control plants (TRV2:00). So the CaDHN3 might act as a positive role in resisting abiotic stresses. This study lays the foundation for further studies into the regulation of their expression under various conditions.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification, Expression Diversication of Dehydrin Gene Family and Characterization of CaDHN3 in Pepper (Capsicum annuum L.)

Hua

et al. 2016

PLoS ONE

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“…The components of the MAPK cascades are generally composed of different gene families, namely MPK, MKK and MEKK families, which have been characterized at the genome-wide level in many plant species including Arabidopsis [8, 9], rice [9, 10], poplar [9], soybean [11], maize [12, 13], tomato [14–16], canola [17], banana [18], apple [19], Gossypium raimondii [20], mulberry [21] and Brachypodium distachyon [22]. The numbers of MPK and MKK families vary greatly across species.…”

Section: Introductionmentioning

confidence: 99%

“…The numbers of MPK and MKK families vary greatly across species. For example, there are 20 MPKs in Arabidopsis [8, 9], 17 in rice [9, 10], 19 in maize [13], 21 in poplar [9], 16 in tomato [14], 12 in canola [17], 10 in mulberry [21], 12 in grapevine [23], 17 in tobacco [24], 38 in soybean [11], 28 in G. raimondii [20] and 16 in B. distachyon [22]. Similarly, 10 MKKs in Arabidopsis [8, 9], 8 in rice [9], 9 in maize [12], 5 in tomato [15, 16] and in canola [17], 11 in soybean [11], 11 in poplar [9], and 12 in B. distachyon [22] were identified.…”

Section: Introductionmentioning

confidence: 99%

Characterization, expression patterns and functional analysis of the MAPK and MAPKK genes in watermelon (Citrullus lanatus)

Song

Dai

et al. 2015

BMC Plant Biol

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BackgroundMitogen-activated protein kinase (MAPK) cascades, which consist of three functionally associated protein kinases, namely MEKKs, MKKs and MPKs, are universal signaling modules in all eukaryotes and have been shown to play critical roles in many physiological and biochemical processes in plants. However, little or nothing is known about the MPK and MKK families in watermelon.ResultsIn the present study, we performed a systematic characterization of the ClMPK and ClMKK families including the identification and nomenclature, chromosomal localization, phylogenetic relationships, ClMPK-ClMKK interactions, expression patterns in different tissues and in response to abiotic and biotic stress and transient expression-based functional analysis for their roles in disease resistance. Genome-wide survey identified fifteen ClMPK and six ClMKK genes in watermelon genome and phylogenetic analysis revealed that both of the ClMPK and ClMKK families can be classified into four distinct groups. Yeast two-hybrid assays demonstrated significant interactions between members of the ClMPK and ClMKK families, defining putative ClMKK2-1/ClMKK6-ClMPK4-1/ClMPK4-2/ClMPK13 and ClMKK5-ClMPK6 cascades. Most of the members in the ClMPK and ClMKK families showed differential expression patterns in different tissues and in response to abiotic (e.g. drought, salt, cold and heat treatments) and biotic (e.g. infection of Fusarium oxysporum f. sp. niveum) stresses. Transient expression of ClMPK1, ClMPK4-2 and ClMPK7 in Nicotiana benthamiana resulted in enhanced resistance to Botrytis cinerea and upregulated expression of defense genes while transient expression of ClMPK6 and ClMKK2-2 led to increased susceptibility to B. cinerea. Furthermore, transient expression of ClMPK7 also led to hypersensitive response (HR)-like cell death and significant accumulation of H2O2 in N. benthamiana.ConclusionWe identified fifteen ClMPK and six ClMKK genes from watermelon and analyzed their phylogenetic relationships, expression patterns and protein-protein interactions and functions in disease resistance. Our results demonstrate that ClMPK1, ClMPK4-2 and ClMPK7 positively but ClMPK6 and ClMKK2-2 negatively regulate the resistance to B. cinerea when transiently expressed in N. benthamiana and that ClMPK7 functions as a regulator of HR-like cell death through modulating the generation of H2O2.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0681-4) contains supplementary material, which is available to authorized users.

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“…The activation of a MAPK cascade often occurs within one to several minutes following stimulation, representing one of the earliest cellular responses to environmental cues. Genome sequencing has revealed numerous plant MAPKs in dicots, monocots, and trees [80][81][82][83][84][85]. The activation of MAPK requires phosphorylation of both threonine and tyrosine residues in a T-x-Y activation loop in subdomain VIII of the catalytic kinase domain.…”

Section: Mapk Cascades In Aba Signalingmentioning

confidence: 99%