Molecular cloning and expression analysis of the replacement histone H3 gene of Italian ryegrass (Lolium multiflorum)

Takahashi, Wataru; Oishi, Hideki; Ikeda, Seishi; Takamizo, Tadashi; Komatsu, Toshinori

doi:10.1016/j.jplph.2005.05.001

Cited by 6 publications

(6 citation statements)

References 28 publications

(28 reference statements)

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“…We found that NgRDR1 cannot be induced by the bacterium Ralstonia solanacearum. Similar results were reported in Arabidopsis, where the T-DNA insertion 36 mutant for AtRDR1 inoculated by bacterium worked similarly to the wild type [43]. However, NgRDR1 can be induced by some fungi and viruses.…”

Section: Discussionsupporting

confidence: 84%

“…Firstly, the Pro1 and Pro2 primers were designed in accordance with the 5 0 end of the genomic sequence and the adaptor-PCR was performed as described previously [37]. Then, we designed primers TF1, TF2, TR1 and TR2 based on the cloned sequence and used the inverse polymerase chain reaction (IPCR) to clone the residual region [36]. At last, the whole obtained sequence of the 5 0 -flanking region was used to design the specific primers Fp and Rp for direct PCR amplification from N. glutinosa genomic DNA.…”

Section: Cis-elementmentioning

confidence: 99%

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NgRDR1, an RNA-dependent RNA polymerase isolated from Nicotiana glutinosa, was involved in biotic and abiotic stresses

Liu

Gao

et al. 2009

Plant Physiology and Biochemistry

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Section: Discussionsupporting

confidence: 84%

Section: Cis-elementmentioning

confidence: 99%

NgRDR1, an RNA-dependent RNA polymerase isolated from Nicotiana glutinosa, was involved in biotic and abiotic stresses

Liu

Gao

et al. 2009

Plant Physiology and Biochemistry

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“…Since expression levels of ZBD1 in the transgenic plants seemed to be low, other promoters should be investigated. The multiple-stress-inducible promoter IRH3prm, which is the 5′-regulatory sequence of a replacement histone H3 gene of Italian ryegrass (Takahashi et al 2006), is a possible candidate.…”

Section: Discussionmentioning

confidence: 99%

Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

Takahashi

Oishi²,

Ebina

et al. 2010

Breed. Sci.

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Transgenic Italian ryegrass plants expressing the betaine aldehyde dehydrogenase gene of zoysiagrass (ZBD1) were produced via particle bombardment of embryogenic calluses. Although the growth of both non-transgenic and transgenic plants expressing ZBD1 was inhibited under salt stress, isolated tillers of the transgenic plants showed better re-rooting ability in vitro in the presence of 300 mM NaCl. Chlorophyll fluorescence values of the transgenic plants declined under salt stress, but were significantly higher than those of non-transgenic plants under salt stress, indicating the improved salt stress tolerance of transgenic plants.

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“…One microgram genomic DNA was digested individually at 37°C for 24 h with 7 restriction endonucleases (BglII, EcoRI, EcoRV, HaeIII, HindIII, XbaI and VspI), respectively. The purification and circularization of the digested DNA samples were performed as described previously (Takahashi et al 2006). For cloning of the full-length GhMPK2 promoter, the circularized DNA digested with BglII was used as template in IPCR, employing two pairs of nested primers: P1 and P2, and P3 and P4.…”

Section: Vector Construction and Genetic Transformationmentioning

confidence: 99%

A cotton group C MAP kinase gene, GhMPK2, positively regulates salt and drought tolerance in tobacco

Zhang

et al. 2011

Plant Mol Biol

124

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Mitogen-activated protein kinase (MAPK) cascades play important roles in mediating biotic and abiotic stress responses. In plants, MAPKs are classified into four major groups (A-D) according to their sequence homology and conserved phosphorylation motifs. Compared with well-studied MAPKs in groups A and B, little is known about group C. In this study, we functionally characterised a stress-responsive group C MAPK gene (GhMPK2) from cotton (Gossypium hirsutum). Northern blot analysis indicated that GhMPK2 was induced by abscisic acid (ABA) and abiotic stresses, such as NaCl, PEG, and dehydration. Subcellular localization analysis suggested that GhMPK2 may activate its specific targets in the nucleus. Constitutive overexpression of GhMPK2 in tobacco (Nicotiana tabacum) conferred reduced sensitivity to ABA during both seed germination and vegetative growth. Interestingly, transgenic plants had a decreased rate of water loss and exhibited enhanced drought and salt tolerance. Additionally, transgenic plants showed improved osmotic adjustment capacity, elevated proline accumulation and up-regulated expression of several stress-related genes, including DIN1, Osmotin and NtLEA5. β-glucuronidase (GUS) expression driven by the GhMPK2 promoter was clearly enhanced by treatment with NaCl, PEG, and ABA. These results strongly suggest that GhMPK2 positively regulates salt and drought tolerance in transgenic plants.

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Molecular cloning and expression analysis of the replacement histone H3 gene of Italian ryegrass (Lolium multiflorum)

Cited by 6 publications

References 28 publications

NgRDR1, an RNA-dependent RNA polymerase isolated from Nicotiana glutinosa, was involved in biotic and abiotic stresses

NgRDR1, an RNA-dependent RNA polymerase isolated from Nicotiana glutinosa, was involved in biotic and abiotic stresses

Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

A cotton group C MAP kinase gene, GhMPK2, positively regulates salt and drought tolerance in tobacco

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