Molecular cloning and expression analysis of a cytosolic heat shock protein 70 gene from mud crab Scylla serrata

Fu, Wei; Zhang, Fei-jun; Liao, Miaofei; Liu, Minhai; Zheng, Bin; Yang, Haitao; Zhong, Mingjie

doi:10.1016/j.fsi.2013.02.027

Cited by 27 publications

(18 citation statements)

References 42 publications

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“…In addition, another new study showed a high cytosolic HSP‐70 expression level at both high and low salt concentrations (Fu et al . ) in mud crab ( Scylla serrate ), indicating that HSP‐70 could be a useful tool and a potential biomarker for phylogenetic analysis for stress. Prolactin ( PRL ) is a key hormone vital for osmoregulation in fish by regulating ion entry and water uptake and the levels of PRL in the pituitary gland and plasma ion composition can be changed in response to different water salinities to regulate the hydro‐mineral balance through regulating water uptake and ion retention (Manzon ; Noh et al .…”

Section: Genes Associated With Environmental Stressorsmentioning

confidence: 99%

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

Eissa

Wang

2014

Reviews in Aquaculture

111

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Stress of aquatic animals occurs due to physical and physiological disturbances in the aquatic environment or system when transportation, crowding, handling or changes of physical and chemical factors take place. There are three regulatory systems having a vital role in stress response: the neural, endocrine and immune systems. Fish exhibit multiple genomic and physiological responses to adjust the compensatory or adaptive mechanism that allows them to mitigate the stressors, maintain their haemostasis and survive. In this review, we describe multiple important genes that are associated with responses to environmental and husbandry stressors and that could be used as biomarkers of environmental and husbandry stressors in fish. The described environmental and husbandry stressors include salinity, temperature, hypoxia and hyperoxia, confinement, density and handling. The main role of stress response in aquatic animals is to compensate or adapt their biological systems and arrange the metabolism to afford the energy required by the stressor and a wide array of metabolic processes and pathways are involved. We summarized and discussed highly significant genes in several organs and tissues that are involved and active during this adaption process. The traditional stress biomarkers in some circumstances have some difficulties in interpretation of results and lead to tricky diagnosis and searching and understanding alternative tools is critical for aquaculture, fisheries and fish welfare. Using genomic tools to study the candidate genes associated with stress responses are often unique signatures or imprints of specific stressors and could determine early signs of stressors.

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Section: Genes Associated With Environmental Stressorsmentioning

confidence: 99%

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

Eissa

Wang

2014

Reviews in Aquaculture

111

View full text Add to dashboard Cite

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“…Similarly, in the present study, compared with the control group, MI supplementation significantly improved the activities of AKP and ACP in hepatopancreas after exposed to acute hypoxia stress. Hsp70, as a member of stress‐related protein (Ni et al, ), plays an important role in resisting the detrimental effects caused by environmental stresses and stimulating innate immune system (Fu et al, ; Qian et al, ; Zhou, Wang, He, et al, ). It had the function of repairing partly denatured proteins (Sinha et al, ).…”

Section: Discussionmentioning

confidence: 99%

Hypoxia-induced changes in survival, immune response and antioxidant status of the Pacific white shrimp (Litopenaeus vannamei ) fed with graded levels of dietary myo -inositol

et al. 2018

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A 3‐hr experiment was conducted to investigate the effects of dietary myo‐inositol (MI) supplementation on survival, immune response and antioxidant abilities in Litopenaeus vannamei under acute hypoxia stress. Six practical diets were formulated with supplementation of graded levels (control group 0, 0.1, 0.2, 0.4, 0.8 and 1.6 g/kg dry diet) of MI and were randomly assigned to triplicate groups of L. vannamei (mean weight 0.40 ± 0.00 g) for 8 weeks. Ten healthy shrimp (final mean weight approximately 11–14 g) randomly selected from each tank were exposed to hypoxia stress after feeding trial. After 3‐hr acute hypoxia stress, survival of shrimp fed MI‐supplemented diets (except 0.1 and 0.4 g/kg diets) was significantly increased compared with the control group. Shrimp fed control diet had lower activities of alkaline phosphatase (AKP), acid phosphatase (ACP), total antioxidant capacity (T‐AOC) and glutathione peroxidase (GPX), and higher malondialdehyde (MDA) and protein carbonyl (PC) contents in hepatopancreas than those fed the MI‐supplemented diets. In addition, mRNA expression levels of heat shock protein 70 (Hsp70), catalase (CAT) and penaeidin were significantly differentially regulated in hepatopancreas. In summary, dietary MI supplementation may have a positive effect on improving resistance to acute hypoxia stress of L. vannamei.

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“…In a third study in which C. sapidus was fed TBT-contaminated food, the respiration rate, the expression of P-450 3A (CYP3A), and heat shock proteins (HSPs) in the hepatopancreas increased, indicating that the crabs were stressed by TBT ( 51 ). An active heat shock response, specially with increased HSP70 expression, occurs when crustaceans are exposed to many types of environmental stress such as heat ( 55 – 58 ), metals ( 59 , 60 ), and salinity alterations ( 61 , 62 ). Therefore, increased expression of HSPs could be a useful indicator of BTs/TBT contamination that should be studied in other crustacean species (Figure 2 ).…”

Section: Ots Effects On the Metabolismmentioning

confidence: 99%

Effects of Organotins on Crustaceans: Update and Perspectives

2018

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Organotins (OTs) are considered some of the most toxic chemicals introduced into aquatic environments by anthropogenic activities. They are widely used for agricultural and industrial purposes and as antifouling additives on boat hull’s paints. Even though the use of OTs was banned in 2008, elevated levels of OTs can still be detected in aquatic environments. OTs’ deleterious effects upon wildlife and experimental animals are well documented and include endocrine disruption, immunotoxicity, neurotoxicity, genotoxicity, and metabolic dysfunction. Crustaceans are key members of zooplankton and benthic communities and have vital roles in food chains, so the endocrine-disrupting effects of tributyltin (TBT) on crustaceans can affect other organisms. TBT can disrupt carbohydrate and lipid homeostasis of crustaceans by interacting with retinoid X receptor (RXR) and crustacean hyperglycemic hormone (CHH) signaling. Moreover, it can also interact with other nuclear receptors, disrupting methyl farnesoate and ecdysteroid signaling, thereby altering growth and sexual maturity, respectively. This compound also interferes in cytochrome P450 system disrupting steroid synthesis and reproduction. Crustaceans are also important fisheries worldwide, and its consumption can pose risks to human health. However, some questions remain unanswered. This mini review aims to update information about the effects of OTs on the metabolism, growth, and reproduction of crustaceans; to compare with known effects in mammals; and to point aspects that still needs to be addressed in future studies. Since both macrocrustaceans and microcrustaceans are good models to study the effects of sublethal TBT contamination, novel studies should be developed using multibiomarkers and omics technology.

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Molecular cloning and expression analysis of a cytosolic heat shock protein 70 gene from mud crab Scylla serrata

Cited by 27 publications

References 42 publications

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

Hypoxia-induced changes in survival, immune response and antioxidant status of the Pacific white shrimp (Litopenaeus vannamei ) fed with graded levels of dietary myo -inositol

Effects of Organotins on Crustaceans: Update and Perspectives

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