Molecular Cloning and Evidence for Osmoregulation of the Δ1-Pyrroline-5-Carboxylate Reductase (proC) Gene in Pea (Pisum sativum L.)

Williamson, Cynthia L.; Slocum, Robert D.

doi:10.1104/pp.100.3.1464

Cited by 58 publications

(30 citation statements)

References 35 publications

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“…Genes for the last enzyme of Pro biosynthesis, P5C reductase, were cloned from soybean, Arabidopsis, and pea (Delauney and Verma, 1990;Williamson and Slocum, 1992;Verbruggen et al, 1993), and it was observed that osmotic stress resulted in a similar increase in the P5C reductase message level as were seen for the P5CS message. The observation that salinity or dehydration stress stimulated the accumulation of the transcripts for the Pro-biosynthetic genes has been interpreted to mean that the transcriptional control of these genes is important for the regulation of Pro synthesis by osmotic stress.…”

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confidence: 99%

“…Arabidopsis, pea, and rice, which have been used to probe the importance of transcriptional control for Pro synthesis, are in fact not the best representatives of Pro accumulators. These plants accumulate only approximately 2 to 6 mol Pro/g fresh weight in response to NaCl stress (Williamson and Slocum, 1992;Savouré et al, 1995;Peng et al, 1996;Igarashi et al, 1997). Thus, unless it is highly concentrated in specific subcellular compartments or organelles, Pro at such low overall concentrations would not be expected to be a substantial determinant of the osmotic potential of the whole cells (Blum et al, 1996;Sharp et al, 1996).…”

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confidence: 99%

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Comparative Analysis of the Regulation of Expression and Structures of Two Evolutionarily Divergent Genes for Δ1-Pyrroline-5-Carboxylate Synthetase from Tomato

Fujita

Maggio

Garcia-Rios³

et al. 1998

Plant Physiology

112

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We isolated two tomato (Lycopersicon esculentum) cDNA clones, tomPRO1 and tomPRO2, specifying ⌬ 1 -pyrroline-5-carboxylate synthetase (P5CS), the first enzyme of proline (Pro) biosynthesis. tomPRO1 is unusual because it resembles prokaryotic polycistronic operons (M.G. García-Ríos, T. Fujita, P.C. LaRosa, R.D. Locy, J.M. Clithero, R.A. Bressan, L.N. Csonka [1997] Proc Natl Acad Sci USA 94: 8249-8254), whereas tomPRO2 encodes a fulllength P5CS. We analyzed the accumulation of Pro and the tomPRO1 and tomPRO2 messages in response to NaCl stress and developmental signals. Treatment with 200 mM NaCl resulted in a >60-fold increase in Pro levels in roots and leaves. However, there was a <3-fold increase in the accumulation of the tomPRO2 message and no detectable induction in the level of the tomPRO1 message in response to NaCl stress. Although pollen contained approximately 100-fold higher levels of Pro than other plant tissues, there was no detectable increase in the level of either message in pollen. We conclude that transcriptional regulation of these genes for P5CS is probably not important for the osmotic or pollenspecific regulation of Pro synthesis in tomato. Using restriction fragment-length polymorphism mapping, we determined the locations of tomPRO1 and tomPRO2 loci in the tomato nuclear genome. Sequence comparison suggested that tomPRO1 is similar to prokaryotic P5CS loci, whereas tomPRO2 is closely related to other eukaryotic P5CS genes.

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confidence: 99%

mentioning

confidence: 99%

Comparative Analysis of the Regulation of Expression and Structures of Two Evolutionarily Divergent Genes for Δ1-Pyrroline-5-Carboxylate Synthetase from Tomato

Fujita

Maggio

Garcia-Rios³

et al. 1998

Plant Physiology

112

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“…The expression of reductase (P5CR) is regulated by osmotic potential variation in the cytoplasm (Williamson & Slocum, 1992). An osmotic potential decrease induces the P5C biosynthesis, which results in proline synthesis.…”

Section: Resultsmentioning

confidence: 99%

Free proline accumulation in sugarcane under water restriction and spittlebug infestation

Guimarães

Mutton

et al. 2008

Sci. agric. (Piracicaba, Braz.)

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Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae) has become a key pest in the sugarcane (Saccharum officinarum) fields of center-south Brazil. Although some control technologies have shown to be efficient, the damage promoted by this spittlebug species and its interaction with sugarcane are poorly characterized. At high infestation levels the symptoms are similar to those of severe water restriction. This work was conducted to determine whether the stress promoted by spittlebug infestation can be measured in terms of free proline accumulation. The water restriction tolerance of two sugarcane genotypes was also compared. Two experiments were set up in a greenhouse and arranged in a completely randomized design in a 2 × 2 × 4 factorial, with two cultivars (SP80-1816 and RB72454), two stress levels (control and ten nymphs per plant or 50% water restriction), and four sampling dates. The water deficit caused by spittlebug nymphs sucking xylem sap does not result in proline accumulation, illustrating that there are different mechanisms to sense when the water deficit is caused by insect feeding or water potential variation in root cells. The cultivar RB72454 accumulates more free-proline, and the dry mass accumulation and stalk growth are less affected in this cultivar under water restriction. The levels of compatible solutes probably cannot be used to measure spittlebug infestation stress in sugarcane and RB72454 is more tolerant to water shortage than SP80-1816. Key words: Mahanarva fimbriolata, Saccharum spp., proline accumulation, water deficit ACÚMULO DE PROLINA EM CANA-DE-AÇÚCAR SOB ESTRESSE HÍDRICO OU SUBMETIDA AO ATAQUE DE CIGARRINHA-DAS-RAÍZESRESUMO: A cigarrinha-das-raízes Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae) tornou-se uma praga importante da cultura da cana-de-açúcar na região centro-sul do Brasil. Estratégias de controle têm sido desenvolvidas, mas os danos promovidos por essa praga e sua interação com a cana-de-açúcar ainda são pouco caracterizados. Em altos níveis de infestação da praga, os sintomas nas plantas são muito semelhantes à restrição hídrica severa. Este trabalho foi conduzido com o objetivo de determinar se o estresse promovido pela infestação de ninfas de cigarrinha-das-raízes resulta em acúmulo de prolina livre, além de comparar dois genótipos de cana-de-açúcar quanto à tolerância ao déficit hídrico. Dois experimentos foram conduzidos em delineamento inteiramente casualizado, em esquema fatorial 2 × 2 × 4 com duas cultivares (SP80-1816 e RB72454), dois níveis de estresse (controle e dez ninfas por planta ou 50% de restrição hídrica) e quatro épocas. O déficit hídrico promovido por M. fimbriolata não resultou em acúmulo de prolina, ilustrando a existência de mecanismos distintos para a percepção do estresse hídrico promovido pela praga e pela variação no potencial osmótico nas células das raízes. A cultivar RB72454 acumula maiores teores de prolina livre, e o acúmulo de massa seca e o crescimento dos colmos são menos afetados nessa cultivar sob restrição hídric...

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“…Likewise, Anabaena 7120 has the GXXXA motif starting G235 onward representing the site for NAD(P) attachment. In line with pea, this domain also contains a GTTIAG sequence involved in NAD(P)H-ribose binding indicating that P5CR utilizes NAD(P)H as the co-factor (Williamson and Slocum 1992).…”

Section: Discussionmentioning

confidence: 99%

Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance

Singh

Tiwari

Singh

et al. 2013

Physiol Mol Biol Plants

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Drought is the most important abiotic stress, challenging sustainable agriculture globally. For desiccation being the multigenic trait, a combination of identified genes from the appropriate organism may render crop tolerant to the water stress. Among the compatible solutes, proline plays multifaceted role in counteracting such stress. The genes encoding proline biosynthesizing enzymes, glutamate 5-kinase (G5K), and pyrroline-5-carboxylate reductase (P5CR) from the low-desiccation-tolerant cyanobacterium Anabaena sp. PCC 7120, were cloned and overexpressed in Escherichia coli BL21(DE3) individually. The recombinant E. coli cells harboring G5K, failed to exhibit enhanced desiccation tolerance relative to those with P5CR that showed increased growth/survival over the wild type. This may be ascribed to the overexpression of the reductase gene. Multiple sequence alignment showed P5CR to be conserved in all the organisms. We hypothesize that P5CR gene from high-desiccation-tolerant cyanobacteria may be adopted as the candidate for making transgenic N 2 -fixing cyanobacterium for paddy fields and/or crop development in future.

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Molecular Cloning and Evidence for Osmoregulation of the Δ¹-Pyrroline-5-Carboxylate Reductase (proC) Gene in Pea (Pisum sativum L.)

Abstract: Several cDNA clones encoding A1-pyrroline-5-carboxylate reductase (P5CR, L-proline:NAD[P]+ 5-oxidoreductase, EC 1.5.1.2), which catalyzes the terminal step in proline biosynthesis, were isolated from a pea leaf library screened with a

Cited by 58 publications

References 35 publications

Comparative Analysis of the Regulation of Expression and Structures of Two Evolutionarily Divergent Genes for Δ1-Pyrroline-5-Carboxylate Synthetase from Tomato

Comparative Analysis of the Regulation of Expression and Structures of Two Evolutionarily Divergent Genes for Δ1-Pyrroline-5-Carboxylate Synthetase from Tomato

Free proline accumulation in sugarcane under water restriction and spittlebug infestation

Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance

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