Molecular cloning and differential expression of the glucocorticoid receptorgene in the estuarine tapertail anchovy Coilia nasus

Abstract

ABSTRACT.To understand the regulation of the glucocorticoid receptor gene (Gcr) during loading and transport stress in fish, the Gcr gene of Coilia nasus was cloned. Gcr in C. nasus is expressed strongly in the liver and muscle, and less stronglyin the gills, brain, spleen, intestine, trunk kidney, and head kidney. Gcr expression in both the liver and muscle was increased by loading and transport stress. NaCl reduced the death rate caused by loading and transport stress, and the expression of Gcr in liver and muscle differed significantly between the NaCl and non-NaCl groups. To investigate whether the elevated Gcr transcripts were translated into protein, proteins extracted from the liver and muscle were analyzed. In both tissues, C. nasus GCR protein expression patterns paralleled those of Gcr mRNA during stress.

Integrated Transcriptome and 16S rDNA Analyses Reveal That Transport Stress Induces Oxidative Stress and Immune and Metabolic Disorders in the Intestine of Hybrid Yellow Catfish (Tachysurus fulvidraco♀ × Pseudobagrus vachellii♂)

“…The fish intestine is the main site of the transformation and excretion of xenobiotics, and it plays a crucial role in stress responses and maintaining homeostasis [ 13 ]. Previous studies have demonstrated that transport stress induces a series of stress responses in the intestinal tract [ 14 , 15 ]. In rainbow trout ( Oncorhynchus mykiss ), the abundance of the chaperone protein HSC70 in the epithelia of the intestine was generally higher in transport-stressed fish than in control fish [ 16 ].…”