Molecular cloning and circadian expression profile of insect neuropeptide PDF in black blowfly,Phormia regina

Matsushima, Ayami; Yokotani, Satoru; Lui, Xiaohui; Sumida, Kazunori; Honda, Takeshi; Sato, Seiji; Kaneki, Atsushi; Takeda, Yukimasa; Chuman, Yoshiro; Ozaki, Mamiko; Asai, Daisuke; Nose, Takeru; Onoue, Hitoshi; Ito, Yushi; Tominaga, Yoshiya; Shimohigashi, Yasuyuki; Shimohigashi, Miki

doi:10.1007/bf02442573

Cited by 7 publications

(5 citation statements)

References 23 publications

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“…They also demonstrated that there is no mRNA for the normal PDF (Park and Hall, 1998;Park et al, 2000). We also confirmed that the ordinary Drosophila pdf mRNA is expressed only in the large and small lateral neurons in each optic lobe (Matsushima et al, 2003(Matsushima et al, , 2004. Although it is possible that a faint immunoreactivity of the PDFCa neurons is simply due to crossreactivity against the antibodies used, the results suggest as well that the dorsal brain calyx cells in Drosophila, like the PDFLa outer cells and VA in Gryllus, contain a PDF isoform, and that they lack the ordinary PDF peptide and fail to express the pdf gene.…”

Section: Molecular Outline and Physiological Significance Of Putativesupporting

confidence: 84%

“…Additional antibodies have also been raised against insect PDFs, however. For instance, Park et al (2000) prepared a rat antibody against Drosophila PDF, and we have prepared rabbit polyclonal antibodies against PDF in the housefly Musca domestica Matsushima et al, 2003Matsushima et al, , 2004. For almost all these antibodies, precise epitope structures have yet to be clarified.…”

Section: Characterization Of the Polyclonal Antibodiesmentioning

confidence: 99%

“…We also examined a rabbit polyclonal antibody that was raised against the Musca PDF ( Sato et al, 2002;Matsushima et al, 2003Matsushima et al, , 2004. This polyclonal antibody exhibited high immunoreactivity against Gryllus PDF as well as Musca PDF, but it showed almost no immunoreactivity against Gryllus PDF-GRK (Fig.…”

Section: Characterization Of the Polyclonal Antibodiesmentioning

confidence: 99%

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Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: Immunocytochemical evidence from specific monoclonal antibodies

Honda

Matsushima

Sumida

et al. 2006

J of Comparative Neurology

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Pigment-dispersing factor (PDF) is an 18-mer peptide that acts as a principal neurotransmitter of the insect circadian clock. Our previous study, utilizing anti-Uca beta-PDH polyclonal antibody (pAb) to immunolabel the optic lobe of the cricket Gryllus bimaculatus, suggested the existence of an alternative PDF-like peptide in the outer cells of the first neuropile, or lamina (La), which were much less immunoreactive than the inner cells of the second neuropile, the medulla (Me). To obtain structural information about such a PDF-like peptide, we prepared 10 anti-Gryllus PDF monoclonal (mAb) and pAb antibodies and analyzed their detailed epitope specificities. The PDFMe and PDFLa inner cells and their axonal projections were clearly immunoreactive to all these antibodies, revealing the widespread immunocytochemical organization of the PDF system in the optic lobe, as seen previously with anti-Uca beta-PDH pAb and anti-Gryllus PDF mAb, the epitope structures of which were also clarified in this study. The lamina outer cells, which we found lacked a target pdf mRNA, displayed specific immunoreactivities, indicating that the cells contain a distinct PDF-like peptide possessing both N- and C-terminal structures. These cells were not immunolabeled by some other monoclonal antibodies, however, implying that the PDFLa outer cells have a PDF isoform peptide devoid of Asn at positions 6 and 16. This isoform was also identified in a varicose arborization in the lamina. These results suggest not only the structure of the peptide, but also the possibility of additional functions of this novel PDF isoform.

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Section: Molecular Outline and Physiological Significance Of Putativesupporting

confidence: 84%

Section: Characterization Of the Polyclonal Antibodiesmentioning

confidence: 99%

Section: Characterization Of the Polyclonal Antibodiesmentioning

confidence: 99%

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Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: Immunocytochemical evidence from specific monoclonal antibodies

Honda

Matsushima

Sumida

et al. 2006

J of Comparative Neurology

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“…Moreover, pdf mRNA expression levels showed no diurnal rhythmicity under either long or short days in the head of R. pedestris. Although diurnal and circadian expression of pdf mRNA were reported for A. mellifera (Sumiyoshi et al, 2011), pdf mRNA levels and cytoplasmic PDF immunoreactivity showed no prominent oscillation in many other insects (Park and Hall, 1998;Park et al, 2000;Matsushima et al, 2003;Hassaneen et al, 2011). However, circadian oscillations of immunoreactivity have been reported in the terminals of s-LN v s in D. melanogaster (Park et al, 2000).…”

Section: Research Articlementioning

confidence: 89%

The involvement of the brain region containing pigment-dispersing factor-immunoreactive neurons in the photoperiodic response of the bean bugRiptortus pedestris

Ikeno

Numata

Goto

et al. 2013

Journal of Experimental Biology

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The concept of insect photoperiodism based on a circadian clock has been supported by many studies demonstrating that the behavioural circadian rhythm and the photoperiodic response are driven by the same circadian clock genes. However, the neuronal mechanism of the circadian clock underlying photoperiodism is poorly understood. To examine whether circadian rhythm and photoperiodism share a neuronal mechanism, we focused on the neurons that express neuropeptide pigment-dispersing factor (PDF) in the bean bug, Riptortus pedestris. PDF has been identified as an important regulator of the insect circadian rhythm and is expressed in circadian clock neurons of various insect species. In R. pedestris, PDF immunoreactivity was detected in some clusters of cells and their fibres in the optic lobe and the protocerebrum. cDNA encoding a PDF precursor protein was highly conserved between R. pedestris and many other insects. Differences between day and night were not observed in the immunolabelling intensity in cell bodies of PDFimmunoreactive neurons and pdf mRNA expression levels in the head. Surgical removal of the region containing PDF-immunoreactive cell bodies at the medulla disrupted the photoperiodic regulation of diapause. However, gene suppression of pdf by RNA interference did not affect the photoperiodic response. These results suggest that the region containing PDF-immunoreactive somata is important for the photoperiodic response in R. pedestris, but pdf mRNA expression is probably not required for the response.

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“…Pigment-dispersing factor immunostaining in the terminals of the sLN v s in the dorsal protocerebrum but not within the somata exhibits a daily and circadian cycle with a peak during early morning, which apparently corresponds to the daily locomotor activity of D. melanogaster . Since the amount of pdf mRNA does not exhibit daily cycles Matsushima et al, 2004b), it is likely that the release of pigment-dispersing factor from the dorsal terminals of the s-LN v s is regulated in a circadian fashion.…”

Section: Introductionmentioning

confidence: 99%

Crustacean Pigmentary-Effector Hormones: Chemistry and Functions of RPCH, PDH, and Related Peptides

Rao

2001

Am Zool

113

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Molecular cloning and circadian expression profile of insect neuropeptide PDF in black blowfly,Phormia regina

Cited by 7 publications

References 23 publications

Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: Immunocytochemical evidence from specific monoclonal antibodies

Structural isoforms of the circadian neuropeptide PDF expressed in the optic lobes of the cricket Gryllus bimaculatus: Immunocytochemical evidence from specific monoclonal antibodies

The involvement of the brain region containing pigment-dispersing factor-immunoreactive neurons in the photoperiodic response of the bean bugRiptortus pedestris

Crustacean Pigmentary-Effector Hormones: Chemistry and Functions of RPCH, PDH, and Related Peptides

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