Molecular Cloning and Characterization of Orthologues of <i><scp>NPR</scp>1</i> Gene from Poplar

Shao, Yuanhua; Zhang, Hongmei; He, Hongsheng; Cheng, Beijiu; Xiang, Yan

doi:10.1111/jph.12002

Cited by 10 publications

(6 citation statements)

References 57 publications

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“…PbrNPR1 - like proteins have similar gene structures, domains, and conserved motifs and amino acid residues with NPR1 -like sequences in Arabidopsis , suggesting that their orthologs probably display similar biological functions in Chinese pear, while the difference between the groups specified their function was diversified. Similar results had existed in Oryza sativa , Malus domestica , Populus trichocarpa ( Shao et al, 2013 ; Yuan et al, 2007 ; Zhang et al, 2016 ). These findings indicated that the NPR1 -like genes were highly conserved in numerous plant species.…”

Section: Discussionsupporting

confidence: 83%

Genome-wide identification, evolution, and expression analysis of the NPR1-like gene family in pears

Wei

Zhao

Liu

et al. 2021

PeerJ

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The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) plays a master regulatory role in the salicylic acid (SA) signal transduction pathway and plant systemic acquired resistance (SAR). Members of the NPR1-like gene family have been reported to the associated with biotic/abiotic stress in many plants, however the genome-wide characterization of NPR1-like genes has not been carried out in Chinese pear (Pyrus bretschneideri Reld). In this study, a systematic analysis was conducted on the characteristics of the NPR1-like genes in P. bretschneideri Reld at the whole-genome level. A total nine NPR1-like genes were detected which eight genes were located on six chromosomes and one gene was mapped to scaffold. Based on the phylogenetic analysis, the nine PbrNPR1-like proteins were divided into three clades (Clades I–III) had similar gene structure, domain and conserved motifs. We sorted the cis-acting elements into three clades, including plant growth and development, stress responses, and hormone responses in the promoter regions of PbrNPR1-like genes. The result of qPCR analysis showed that expression diversity of PbrNPR1-like genes in various tissues. All the genes were up-regulated after SA treatment in leaves except for Pbrgene8896. PbrNPR1-like genes showed circadian rhythm and significantly different expression levels after inoculation with Alternaria alternata. These findings provide a solid insight for understanding the functions and evolution of PbrNPR1-like genes in Chinese pear.

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Section: Discussionsupporting

confidence: 83%

Genome-wide identification, evolution, and expression analysis of the NPR1-like gene family in pears

Wei

Zhao

Liu

et al. 2021

PeerJ

View full text Add to dashboard Cite

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“…The nuclear localization of NPR1 is essential for the expression of PR genes (Despres et al, 2000; Kinkema et al, 2000; Mou et al, 2003). Preceding cellular oxidative stress NPR1 is primarily found within the cytoplasm in an oligomeric form, though some notable exceptions exist (Kinkema et al, 2000; Mou et al, 2003; Le Henanff et al, 2009; Zhang et al, 2010c; Maier et al, 2011; Peraza-Echeverria et al, 2012; Shao et al, 2013). The production of SA and subsequent oxidative stress decreases cellular reduction potential enforcing an increased production of reducing agents (Mou et al, 2003).…”

Section: Monomerization Of Npr1mentioning

confidence: 99%

The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) and Related Family: Mechanistic Insights in Plant Disease Resistance

2019

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The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) and related NPR1-like proteins are a functionally similar, yet surprisingly diverse family of transcription co-factors. Initially, NPR1 in Arabidopsis was identified as a positive regulator of systemic acquired resistance (SAR), paralogs NPR3 and NPR4 were later shown to be negative SAR regulators. The mechanisms involved have been the subject of extensive research and debate over the years, during which time a lot has been uncovered. The known roles of this protein family have extended to include influences over a broad range of systems including circadian rhythm, endoplasmic reticulum (ER) resident proteins and the development of lateral organs. Recently, important advances have been made in understanding the regulatory relationship between members of the NPR1-like protein family, providing new insight regarding their interactions, both with each other and other defense-related proteins. Most importantly the influence of salicylic acid (SA) on these interactions has become clearer with NPR1, NPR3, and NPR4 being considered bone fide SA receptors. Additionally, post-translational modification of NPR1 has garnered attention during the past years, adding to the growing regulatory complexity of this protein. Furthermore, growing interest in NPR1 overexpressing crops has provided new insights regarding the role of NPR1 in both biotic and abiotic stresses in several plant species. Given the wealth of information, this review aims to highlight and consolidate the most relevant and influential research in the field to date. In so doing, we attempt to provide insight into the mechanisms and interactions which underly the roles of the NPR1-like proteins in plant disease responses.

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“…In addition to agronomic and horticultural applications, AtNPR1 orthologs have been isolated and shown to confer effects in trees. Shao et al 76 isolated two AtNPR1 homologous genes ( PtNPR1 . 1 and PtNPR1 .…”

Section: Use Of Atnpr1 and Its Orthologs For Crop mentioning

confidence: 99%

NPR1 as a transgenic crop protection strategy in horticultural species

et al. 2018

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The NPR1 (NONEXPRESSOR OF PATHOGENESIS RELATED GENES1) gene has a central role in the long-lasting, broad-spectrum defense response known as systemic acquired resistance (SAR). When overexpressed in a transgenic context in Arabidopsis thaliana, this gene enhances resistance to a number of biotic and abiotic stresses. Its position as a key regulator of defense across diverse plant species makes NPR1 a strong candidate gene for genetic engineering disease and stress tolerance into other crops. High-value horticultural crops face many new challenges from pests and pathogens, and their emergence exceeds the pace of traditional breeding, making the application of NPR1-based strategies potentially useful in fruit and vegetable crops. However, plants overexpressing NPR1 occasionally present detrimental morphological traits that make its application less attractive. The practical utility of NPR-based approaches will be a balance of resistance gains versus other losses. In this review, we summarize the progress on the understanding of NPR1-centered applications in horticultural and other crop plants. We also discuss the effect of the ectopic expression of the A. thaliana NPR1 gene and its orthologs in crop plants and outline the future challenges of using NPR1 in agricultural applications.

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Molecular Cloning and Characterization of Orthologues of NPR1 Gene from Poplar

Cited by 10 publications

References 57 publications

Genome-wide identification, evolution, and expression analysis of the NPR1-like gene family in pears

Genome-wide identification, evolution, and expression analysis of the NPR1-like gene family in pears

The NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) and Related Family: Mechanistic Insights in Plant Disease Resistance

NPR1 as a transgenic crop protection strategy in horticultural species

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