Molecular cloning and characterization of two nicotinic acetylcholine receptor β subunit genes from <i>Apis cerana cerana</i>

Yu, Xiaoli; Wang, Mian; Kang, Mingjiang; Liu, Li; Guo, Xingqi; Xu, Baohua

doi:10.1002/arch.20432

Cited by 16 publications

(17 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results demonstrated that pea aphid nAChR subunits were differentially expressed, first between developmental stages, as previously demonstrated in Drosophila and Apis cerana cerana [35], [37], [38], and also according to the neonicotinoid exposure. Neonicotinoid sensitivity could then be dependent on either physiological status and/or environmental conditions in the pea aphid.…”

Section: Discussionsupporting

confidence: 85%

“…Moreover, recent studies demonstrated that nAChR subunit expression level was associated to neonicotinoid sensitivity [34], [35]. For example, a decrease in Accβ1 and Accβ2 subunit expression in the Asiatic honey bee Apis cerana was described after IMI exposure [35]. Our results also demonstrated that neonicotinoid toxicity was associated with specific nAChR subunit regulation.…”

Section: Discussionsupporting

confidence: 73%

“…Mutation of arginine to threonine at position 81 in this subunit induced an increase of resistance against neonicotinoids [31], [32], [33]. Moreover, recent studies demonstrated that nAChR subunit expression level was associated to neonicotinoid sensitivity [34], [35]. For example, a decrease in Accβ1 and Accβ2 subunit expression in the Asiatic honey bee Apis cerana was described after IMI exposure [35].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Neonicotinoid Binding, Toxicity and Expression of Nicotinic Acetylcholine Receptor Subunits in the Aphid Acyrthosiphon pisum

et al. 2014

View full text Add to dashboard Cite

Neonicotinoid insecticides act on nicotinic acetylcholine receptor and are particularly effective against sucking pests. They are widely used in crops protection to fight against aphids, which cause severe damage. In the present study we evaluated the susceptibility of the pea aphid Acyrthosiphon pisum to the commonly used neonicotinoid insecticides imidacloprid (IMI), thiamethoxam (TMX) and clothianidin (CLT). Binding studies on aphid membrane preparations revealed the existence of high and low-affinity binding sites for [3H]-IMI (Kd of 0.16±0.04 nM and 41.7±5.9 nM) and for the nicotinic antagonist [125I]-α-bungarotoxin (Kd of 0.008±0.002 nM and 1.135±0.213 nM). Competitive binding experiments demonstrated that TMX displayed a higher affinity than IMI for [125I]-α-bungarotoxin binding sites while CLT affinity was similar for both [125I]-α-bungarotoxin and [3H]-IMI binding sites. Interestingly, toxicological studies revealed that at 48 h, IMI (LC50 = 0.038 µg/ml) and TMX (LC50 = 0.034 µg/ml) were more toxic than CLT (LC50 = 0.118 µg/ml). The effect of TMX could be associated to its metabolite CLT as demonstrated by HPLC/MS analysis. In addition, we found that aphid larvae treated either with IMI, TMX or CLT showed a strong variation of nAChR subunit expression. Using semi-quantitative PCR experiments, we detected for all insecticides an increase of Apisumα10 and Apisumβ1 expressions levels, whereas Apisumβ2 expression decreased. Moreover, some other receptor subunits seemed to be differently regulated according to the insecticide used. Finally, we also demonstrated that nAChR subunit expression differed during pea aphid development. Altogether these results highlight species specificity that should be taken into account in pest management strategies.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Neonicotinoid Binding, Toxicity and Expression of Nicotinic Acetylcholine Receptor Subunits in the Aphid Acyrthosiphon pisum

et al. 2014

View full text Add to dashboard Cite

show abstract

“…It is known that different nAChR sub-types have different pharmacological properties [42]. Thus, species could differ in their sensitivity to specific neonicotinoid compounds by having differential composition and populations of nAChR sub-types [43,44], or by differential responses in gene expression of nAChR subunits to pesticide exposure [45,46].…”

Section: Discussionmentioning

confidence: 99%

Chronic neonicotinoid pesticide exposure and parasite stress differentially affects learning in honeybees and bumblebees

Piiroinen¹,

Goulson²

2016

Proc. R. Soc. B.

View full text Add to dashboard Cite

Learning and memory are crucial functions which enable insect pollinators to efficiently locate and extract floral rewards. Exposure to pesticides or infection by parasites may cause subtle but ecologically important changes in cognitive functions of pollinators. The potential interactive effects of these stressors on learning and memory have not yet been explored. Furthermore, sensitivity to stressors may differ between species, but few studies have compared responses in different species. Here, we show that chronic exposure to fieldrealistic levels of the neonicotinoid clothianidin impaired olfactory learning acquisition in honeybees, leading to potential impacts on colony fitness, but not in bumblebees. Infection by the microsporidian parasite Nosema ceranae slightly impaired learning in honeybees, but no interactive effects were observed. Nosema did not infect bumblebees (3% infection success). Nevertheless, Nosema-treated bumblebees had a slightly lower rate of learning than controls, but faster learning in combination with neonicotinoid exposure. This highlights the potential for complex interactive effects of stressors on learning. Our results underline that one cannot readily extrapolate findings from one bee species to others. This has important implications for regulatory risk assessments which generally use honeybees as a model for all bees.

show abstract

“…Imidacloprid and its metabolites act by binding to nicotinic acetylcholine receptors (nAChR) on honey bee neurons23. In A. cerana , imidacloprid binds to a nAChR receptor24. Imidacloprid consequently exerts a broad suite of sublethal neural effects: brain cell death25, impaired motor function2627, reduced food uptake28, decreased foraging29, diminished hive entrance activity30, reduced predator avoidance31, impaired navigation back to the nest3233, and compromised learning34.…”

mentioning

confidence: 99%

A neonicotinoid impairs olfactory learning in Asian honey bees (Apis cerana) exposed as larvae or as adults

Tan

Chen

Dong

et al. 2015

Sci Rep

View full text Add to dashboard Cite

Xenobiotics such as the neonicotinoid pesticide, imidacloprid, are used globally, but their effects on native bee species are poorly understood. We studied the effects of sublethal doses of imidacloprid on olfactory learning in the native honey bee species, Apis cerana, an important pollinator of agricultural and native plants throughout Asia. We provide the first evidence that imidacloprid can impair learning in A. cerana workers exposed as adults or as larvae. Adults that ingested a single imidacloprid dose as low as 0.1 ng/bee had significantly reduced olfactory learning acquisition, which was 1.6-fold higher in control bees. Longer-term learning (1-17 h after the last learning trial) was also impaired. Bees exposed as larvae to a total dose of 0.24 ng/bee did not have reduced survival to adulthood. However, these larval-treated bees had significantly impaired olfactory learning when tested as adults: control bees exhibited up to 4.8-fold better short-term learning acquisition, though longer-term learning was not affected. Thus, sublethal cognitive deficits elicited by neonicotinoids on a broad range of native bee species deserve further study.

show abstract

Molecular cloning and characterization of two nicotinic acetylcholine receptor β subunit genes from Apis cerana cerana

Cited by 16 publications

References 33 publications

Neonicotinoid Binding, Toxicity and Expression of Nicotinic Acetylcholine Receptor Subunits in the Aphid Acyrthosiphon pisum

Neonicotinoid Binding, Toxicity and Expression of Nicotinic Acetylcholine Receptor Subunits in the Aphid Acyrthosiphon pisum

Chronic neonicotinoid pesticide exposure and parasite stress differentially affects learning in honeybees and bumblebees

A neonicotinoid impairs olfactory learning in Asian honey bees (Apis cerana) exposed as larvae or as adults

Contact Info

Product

Resources

About