Molecular Cloning and Characterization of the MouseKin17Gene Coding for a Zn-Finger Protein That Preferentially Recognizes Bent DNA

Tissier, Agnès; Kannouche, Patricia; Mauffrey, Philippe; Allemand, Isabelle; Frelat, G.; Devoret, Raymond; Angulo, Jaime F.

doi:10.1006/geno.1996.0623

Cited by 20 publications

(16 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More distant homologs have been found throughout the ␣-, ␤-, and ␥-proteobacteria, with several species encoding multiple H-NS paralogs (Tendeng and Bertin 2003). Interestingly, kin17/btcd, a zinc-finger DNA-binding protein in mammals, has no significant primary amino acid homology with H-NS yet can complement an hns mutation in E. coli (Timchenko et al 1996;Tissier et al 1996). The kin17 protein is stress-activated and appears to be involved in DNA replication in mammalian cells (Masson et al 2003).…”

Section: How Widespread Is Xenogeneic Silencing?mentioning

confidence: 99%

Silencing of xenogeneic DNA by H-NS—facilitation of lateral gene transfer in bacteria by a defense system that recognizes foreign DNA

Navarre¹,

McClelland²,

Libby³

et al. 2007

Genes Dev.

270

261

View full text Add to dashboard Cite

Lateral gene transfer has played a prominent role in bacterial evolution, but the mechanisms allowing bacteria to tolerate the acquisition of foreign DNA have been incompletely defined. Recent studies show that H-NS, an abundant nucleoid-associated protein in enteric bacteria and related species, can recognize and selectively silence the expression of foreign DNA with higher adenine and thymine content relative to the resident genome, a property that has made this molecule an almost universal regulator of virulence determinants in enteric bacteria. These and other recent findings challenge the ideas that curvature is the primary determinant recognized by H-NS and that activation of H-NS-silenced genes in response to environmental conditions occurs through a change in the structure of H-NS itself. Derepression of H-NS-silenced genes can occur at specific promoters by several mechanisms including competition with sequence-specific DNA-binding proteins, thereby enabling the regulated expression of foreign genes. The possibility that microorganisms maintain and exploit their characteristic genomic GC ratios for the purpose of self/non-self-discrimination is discussed.

show abstract

Section: How Widespread Is Xenogeneic Silencing?mentioning

confidence: 99%

Silencing of xenogeneic DNA by H-NS—facilitation of lateral gene transfer in bacteria by a defense system that recognizes foreign DNA

Navarre¹,

McClelland²,

Libby³

et al. 2007

Genes Dev.

270

261

View full text Add to dashboard Cite

show abstract

“…This gene is located on band A of chromosome 2 in mouse (Angulo et al, 1991) and on human chromosome 10 at p15-14. In adult mouse tissues this gene is ubiquitously expressed at a low level (Tissier et al, 1996). KIN17 is a nuclear protein of 45 kDa conserved from yeast to human which is up-regulated after UV and γ irradiation Blattner et al, 2000;Kannouche et al, 1998;Masson et al, 2001;Masson et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

KIN17 encodes an RNA-binding protein and is expressed during mouse spermatogenesis

Pinon‐Lataillade

Masson

Bernardino-Sgherri

et al. 2004

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

Genotoxic agents deform DNA structure thus eliciting a complex genetic response allowing recovery and cell survival. The Kin17 gene is up-regulated during this response. This gene encodes a conserved nuclear protein that shares a DNA-binding domain with the bacterial RecA protein. The KIN17 protein binds DNA and displays enhanced expression levels in proliferating cultured cells, suggesting a role in nuclear metabolism. We investigated this by studying the expression profile of KIN17 protein during mouse spermatogenesis. As expected, the expression level of Kin17 is higher in proliferating than in differentiated cells. KIN17 is selectively extracted from this tissue by detergents and a fraction was tightly associated with the nuclear matrix. Germinal cells ubiquitously express Kin17 and the protein is located mainly in the nucleus except in elongated spermatids where cytoplasmic staining is also observed. Sertoli and germ cells that are no longer mitotically active express KIN17, suggesting a general role in all testicular cell types. In adult testis a significant proportion of KIN17 co-purifies with polyadenylated RNA. KIN17 directly binds RNA, preferentially poly(G) and poly(U) homopolymers. These results together with the identification of KIN17 as a component of the human spliceosome indicate that this protein may participate in RNA processing.

show abstract

“…Overexpression of Kin17 seems to be toxic for mammalian cells (40). Bacterially produced Kin17 binds to DNA (64,65). The physiologic function of Kin17 is unknown as yet.…”

mentioning

confidence: 99%

UV-Induced Stabilization of c-fos and Other Short-Lived mRNAs

Blattner

Kannouche²,

Litfin

et al. 2000

Molecular and Cellular Biology

Self Cite

View full text Add to dashboard Cite

Molecular Cloning and Characterization of the MouseKin17Gene Coding for a Zn-Finger Protein That Preferentially Recognizes Bent DNA

Cited by 20 publications

References 1 publication

Silencing of xenogeneic DNA by H-NS—facilitation of lateral gene transfer in bacteria by a defense system that recognizes foreign DNA

Silencing of xenogeneic DNA by H-NS—facilitation of lateral gene transfer in bacteria by a defense system that recognizes foreign DNA

KIN17 encodes an RNA-binding protein and is expressed during mouse spermatogenesis

UV-Induced Stabilization of c-fos and Other Short-Lived mRNAs

Contact Info

Product

Resources

About