Molecular cloning and characterization of F9 fimbriae from a uropathogenic

Molecular cloning of the F8 fimbrial antigen from Escherichia coli

“…The size of the F8 fimbrial subunit was found to be nearly 20 kd, which is in good agreement with the Mr value of 19.7 kd published by Moser et al (submitted for publication) and is within the range of 15-29 kd that has been described for other fimbriae [8][9][10][11][12][13][14]. No differences in size could be seen between F8 fimbriae isolated from the wild-type isolate and from the K-12 clones harboring the cloned F8 DNA.…”

“…5.0 and 8.5 kb [8][9][10][11][12][13][14]23]. Therefore, it is suggested that the region coding for F8 fimbriae is smaller than the 12-kb segment inserted into pBR322.…”

“…On the basis of the restriction sites, no similarities were revealed between the cloned Sfimbrial antigen ( sfa) determinant and the F8 genes [23). Some restriction sites, however, such as a C/ai site and a Kpni site, seem tobe conserved in different cloned P-fimbrial determinants coding for F8, F9, F11, F7 1 , F13 or F 'Clegg' [8][9][10][11][12][13][14]. It therefore appears that a 6.0-kb DNA fragment which resulted from a Kpni-Hindiii double cleavage of pANN921 carried most of the F8 coding region.…”

“…As a probe, a 6.0-kb Kpn 1-Hindlll fragment was chosen. As judged from the restriction maps of other cloned F determinants, which share sequence homology [8][9][10][11][12][13][14], this fragment should carry most of the intemal F8 coding region.· As shown in Fig. 3, identical Pst I fragments were obtained from the chromosomal and the plasmid DNA.…”

“…p fimbriae can be divided into serologically distinct groups, termed F7-F13, but all recognize the a-~r.Gal (1-4)-ß-n-Gal region of globotetraosylceramide and trihexosylceramide, which are an tigens of the human blood group P system [7]. Some of these specific F-antigens have been cloned very receritly [8][9][10][11][12][13] and one of them, the Fl3 fimbria (also called pap ), has been studied in detail (14]. Here we describe the molecular cloning and charac~eri zation of the F8 fimbrial antigen from an 018: K5: H5: F1C, F8 wild-type isolate 2980.…”