Molecular cloning and characterization of bile salt hydrolase inLactobacillus casei Zhang

Zhang, Wen Yi; Wu, Ri; Sun, Zhihong; Sun, Tian Song; Meng, He; Zhang, He Ping

doi:10.1007/bf03179214

Cited by 19 publications

(9 citation statements)

References 28 publications

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“…On the other hand, the results of the qRT-PCR experiments indicated that proteins involved in cell protection and modification of the cell membrane as well as key components of central metabolism may play an important role in bile salts resistance of L. casei Zhang. However, none of the putative bile salt hydrolase homologs in L. casei Zhang reported in previous studies (Zhang et al, 2009) showed differential expression during the bile treatments as revealed by 2-DE in this study.…”

Section: Discussioncontrasting

confidence: 75%

Effect of bile salts stress on protein synthesis of Lactobacillus casei Zhang revealed by 2-dimensional gel electrophoresis

Sun

et al. 2010

Journal of Dairy Science

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Lactobacillus casei Zhang, isolated from koumiss in Inner Mongolia of China, is known from previous findings to be tolerant to bile salts. Bile salts secreted by mammals act as a natural antibacterial barrier and may serve as a component of innate immunity, as they have limited antagonistic effect against resident microflora. In this work, we compared the growth and protein expression patterns of L. casei Zhang with and without bile salts. Twenty-six proteins were found to be differentially expressed using 2-dimensional gel electrophoresis. Peptide mass fingerprinting was used to identify these proteins. Further verification by using real-time, quantitative reverse transcription-PCR and bioinformatics analysis showed that the implicated pathways are involved with a complex physiological response under bile salts stress, particularly including cell protection (DnaK and GroEL), modifications in cell membranes (NagA, GalU, and PyrD), and key components of central metabolism (PFK, PGM, CysK, LuxS, PepC, and EF-Tu). These results provide insight on the protein expression pattern of L. casei under bile salts stress and offer a new perspective for the molecular mechanisms involved in stress tolerance and adaptation of bacteria.

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Section: Discussioncontrasting

confidence: 75%

Effect of bile salts stress on protein synthesis of Lactobacillus casei Zhang revealed by 2-dimensional gel electrophoresis

Sun

et al. 2010

Journal of Dairy Science

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“…A concentration of 0.5% (w/v) bile salts resulted in up to a 4-fold down-regulation in the expression of bsh gene in all strains compared with its expression in the control (without bile salts). This result differs considerably from the finding of Zhang et al (2009) who reported that L. casei strain Zhang exhibited a more than one fold change in expression when grown with 0.5% (w/v) bile salts.…”

Section: Expression Of the Homologous Bsh Genescontrasting

confidence: 99%

Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus

et al. 2020

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Abstract. Widodo, Nuratri Y, Sukarno AS, Ahyuningsih TD. 2020. Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus. Biodiversitas 21: 5901-5905. Bile salt hydrolase (BSH) has been considered an important enzyme that is produced in response to bile stress. The bsh gene reportedly encodes BSH in lactic acid bacteria. Thus, the aim of this study was to detect and measure the changes in bsh gene expression in Pediococcus pentosaceus M103, Lactobacillus casei AP, Lactobacillus casei AG, and Lactobacillus paracasei M104. A BSH primer was used in amplifying the bacterial genomic DNA, and the obtained amplicon was then sequenced and analyzed in order to detect genes homologous to bsh. Changes in expression levels were measured in three concentrations of bile salts: 0.1%, 0.3%, and 0.5% (w/v). The BSH primer produced amplicons 850 to 950 kb in length. Amino acid sequences from the amplicons were determined to be similar to the product sequence of BSH. The level of expression of the bsh gene was altered in every concentration of bile salts in the growth medium with bsh genes from P. pentosaceus M103 were induced by bile salts at concentrations of 0.1% and 0.3% (w/v). We conclude that P. pentosaceus M103 may make suitable probiotic candidates that can tolerate conjugated bile acids.

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“…Bile salt hydrolase (BSH) is a protein expressed by bacteria commonly found in the intestinal tract that can deconjugate bile salts and also have the added positive effect of lowering serum cholesterol levels and reducing the risk of obesity and arthrosclerosis in the host ( Wang et al, 2011 ). BSH activity has been found in L. casei but is not always present ( Gilliland and Speck, 1977 ; Zhang et al, 2009 ; Wang et al, 2011 ). There is conflicting evidence whether or not BSH increases tolerance to bile salts with many studies finding a correlation ( Moser and Savage, 2001 ; Wang et al, 2011 ) while many others have failed to find correlation between BSH activity and bile tolerance ( Begley et al, 2005 ; Bustos et al, 2012 ).…”

Section: Bile Stressmentioning

confidence: 99%

The Lactobacillus casei Group: History and Health Related Applications

et al. 2018

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The Lactobacillus casei group (LCG), composed of the closely related Lactobacillus casei, Lactobacillus paracasei, and Lactobacillus rhamnosus are some of the most widely researched and applied probiotic species of lactobacilli. The three species have been extensively studied, classified and reclassified due to their health promoting properties. Differentiation is often difficult by conventional phenotypic and genotypic methods and therefore new methods are being continually developed to distinguish the three closely related species. The group remain of interest as probiotics, and their use is widespread in industry. Much research has focused in recent years on their application for health promotion in treatment or prevention of a number of diseases and disorders. The LCG have the potential to be used prophylactically or therapeutically in diseases associated with a disturbance to the gut microbiota. The group have been extensively researched with regard to stress responses, which are crucial for their survival and therefore application as probiotics.

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Molecular cloning and characterization of bile salt hydrolase inLactobacillus casei Zhang

Cited by 19 publications

References 28 publications

Effect of bile salts stress on protein synthesis of Lactobacillus casei Zhang revealed by 2-dimensional gel electrophoresis

Effect of bile salts stress on protein synthesis of Lactobacillus casei Zhang revealed by 2-dimensional gel electrophoresis

Short Communication: Detection and expression analysis of the bile salt hydrolase gene in Pediococcus and Lactobacillus

The Lactobacillus casei Group: History and Health Related Applications

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