Molecular cloning and characterization of cDNAs of the superoxide dismutase gene family in the resurrection plant Haberlea rhodopensis

Apostolova, Elena; Rashkova, Maya; Anachkov, Nikolay; Denev, Iliya; Toneva, Valentina; Minkov, Ivan; Yahubyan, Galina

doi:10.1016/j.plaphy.2012.03.015

Cited by 18 publications

(10 citation statements)

References 31 publications

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“…Generally, the CuZnSOD contains three conserved motifs defined as CuZnSOD signature motifs; these signature motifs KAVAVL, F(L)HL(I)HQ(A)F(L)GDTTNGC, and GNAGGRLACGVV(I)GL were also identified in the CeCSD1 and CeCSD2 proteins (Figures and ). These were in accordance with previous findings, indicating that these sites and motifs are essential for the structure and function of CuZnSOD and may be involved in stabilizing SOD conformation under harsh environmental conditions …”

Section: Resultssupporting

confidence: 93%

“…In addition, the copper and zinc binding residues, and the two cysteines that form a single disulfide bridge, were conserved in both genes (Figure C) and in other reported CuZnSODs . Generally, the CuZnSOD contains three conserved motifs defined as CuZnSOD signature motifs; these signature motifs KAVAVL, F(L)HL(I)HQ(A)F(L)GDTTNGC, and GNAGGRLACGVV(I)GL were also identified in the CeCSD1 and CeCSD2 proteins (Figures and ).…”

Section: Resultsmentioning

confidence: 69%

“…To date, only two groups of CuZnSODs have been reported: chloroplastic CuZnSOD with an N‐terminal signal peptide for secretion, and cytosolic forms without the signal peptide . CuZnSODs have been isolated and characterized from animals and plants and have been used to enhance resistance against oxidative stress . However, the two isoforms have not been studied sufficiently in microalgae.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Wang

2019

Environmental Toxicology

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Superoxide dismutase (SOD) acts as the first line of defense against reactive oxygen species (ROS) within cells. In the present study, we determined two novel CuZnSOD genes (designated as CeCSD1 and CeCSD2) from the toxicity-testing freshwater algae Closterium ehrenbergii and examined their structural features, phylogenetic relationships, and gene expression under exposure to different metals. Putative CeCSD1 (204 aa, 20.6 kDa) and CeCSD2 (155 aa, 15.3 kDa) proteins had conserved CuZnSOD family motifs and metal (Cu, Zn) binding sites, but different N-terminus structures, that is, CeCSD1 has a signal peptide to chloroplasts. Phylogenetic analysis of each protein revealed that C. ehrenbergii was well clustered with other green algae and plants. Real-time PCR results showed that the gene expression obviously increased with heavy metal exposure. In addition, excess copper considerably increased the SOD activity and ROS generation but decreased the photosynthetic efficiency in treated cells. These results suggest that CeCSDs are involved in the antioxidant defense system and can be regarded as potential biomarkers for monitoring metal contaminants in aquatic environments. K E Y W O R D SCeCSD, Closterium ehrenbergii, gene expression, heavy metals, ROS

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Wang

2019

Environmental Toxicology

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“…According to Apostolova et al . (), the distribution of H. rhodopensis became narrower at the beginning of the Quaternary in areas in which the microclimate showed similarities (being warm and wet) to that during the Tertiary period.…”

Section: Discussionmentioning

confidence: 87%

Complex fine-scale phylogeographical patterns in a putative refugial region forFagus sylvatica(Fagaceae)

et al. 2014

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Broad-scale plastid (chloroplast) DNA studies of beech (Fagus sylvatica) populations suggest the existence of glacial refugia and introgression zones in south-eastern Europe. We choose a possible refugium of beech in northern Greece, Mt. Paggeo, which hosts a private plastid haplotype for beech, to conduct a fine-scale genetic study. We attempt to confirm or reject the hypothesis of the existence of a small-scale refugium and to gain an understanding of the ecological and topographical factors affecting the spatial distribution of plastid haplotypes in the area. Our results reveal a high haplotype diversity on Mt. Paggeo, but the overall distribution of haplotypes shows no significant correlation with the ecological characteristics of the beech forests. However, the private haplotype is found at high frequencies in beech forests located in or near ravines, having a high spatial overlap with a relict vegetation type occurring in ecological conditions found mainly in ravines. This result emphasizes the importance of topography in the existence of glacial refugia in the wider area. Furthermore, haplotypes originating from two more widespread beech lineages in Greece are found on Mt. Paggeo, indicating a possible mixing of populations originating from a local refugium with populations from remote refugia that possibly migrated into the area after the last glaciation.

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“…Despite Haberlea's potential in plant biotechnology and biomedical science, the genome of this species remains unsequenced and very limited information is currently available concerning its genes (Apostolova et al, 2012 ; Georgieva et al, 2012 ). Recently, the first comprehensive transcriptome and metabolite profiling study of H. rhodopensis indicated the types of genes present in Haberlea and how they are expressed during dehydration and rehydration (Gechev et al, 2013a ).…”

Section: Discussionmentioning

confidence: 99%

Comparative metabolic profiling of Haberlea rhodopensis, Thellungiella halophyla, and Arabidopsis thaliana exposed to low temperature

Benina¹,

Obata²,

Mehterov³

et al. 2013

Front. Plant Sci.

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Haberlea rhodopensis is a resurrection species with extreme resistance to drought stress and desiccation but also with ability to withstand low temperatures and freezing stress. In order to identify biochemical strategies which contribute to Haberlea's remarkable stress tolerance, the metabolic reconfiguration of H. rhodopensis during low temperature (4°C) and subsequent return to optimal temperatures (21°C) was investigated and compared with that of the stress tolerant Thellungiella halophyla and the stress sensitive Arabidopsis thaliana. Metabolic analysis by GC-MS revealed intrinsic differences in the metabolite levels of the three species even at 21°C. H. rhodopensis had significantly more raffinose, melibiose, trehalose, rhamnose, myo-inositol, sorbitol, galactinol, erythronate, threonate, 2-oxoglutarate, citrate, and glycerol than the other two species. A. thaliana had the highest levels of putrescine and fumarate, while T. halophila had much higher levels of several amino acids, including alanine, asparagine, beta-alanine, histidine, isoleucine, phenylalanine, serine, threonine, and valine. In addition, the three species responded differently to the low temperature treatment and the subsequent recovery, especially with regard to the sugar metabolism. Chilling induced accumulation of maltose in H. rhodopensis and raffinose in A. thaliana but the raffinose levels in low temperature exposed Arabidopsis were still much lower than these in unstressed Haberlea. While all species accumulated sucrose during chilling, that accumulation was transient in H. rhodopensis and A. thaliana but sustained in T. halophila after the return to optimal temperature. Thus, Haberlea's metabolome appeared primed for chilling stress but the low temperature acclimation induced additional stress-protective mechanisms. A diverse array of sugars, organic acids, and polyols constitute Haberlea's main metabolic defence mechanisms against chilling, while accumulation of amino acids and amino acid derivatives contribute to the low temperature acclimation in Arabidopsis and Thellungiella. Collectively, these results show inherent differences in the metabolomes under the ambient temperature and the strategies to respond to low temperature in the three species.

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Molecular cloning and characterization of cDNAs of the superoxide dismutase gene family in the resurrection plant Haberlea rhodopensis

Cited by 18 publications

References 31 publications

Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Molecular characterization and expression analysis of copper‐zinc superoxide dismutases from the freshwater alga Closterium ehrenbergii under metal stress

Complex fine-scale phylogeographical patterns in a putative refugial region forFagus sylvatica(Fagaceae)

Comparative metabolic profiling of Haberlea rhodopensis, Thellungiella halophyla, and Arabidopsis thaliana exposed to low temperature

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