Molecular cloning and characterization of a rat homolog of CAP, the adenylyl cyclase-associated protein from Saccharomyces cerevisiae

We previously identified human CAP, a homolog of the yeast adenylyl cyclase-associated protein. Previous studies suggest that the N-terminal and C-terminal domains of CAP have distinct functions. We have explored the interactions of human CAP with various proteins. First, by performing yeast two-hybrid screens, we have identified peptides from several proteins that interact with the C-terminal and/or the N-terminal domains of human CAP. These peptides include regions derived from CAP and BAT3, a protein with unknown function. We have further shown that MBP fusions with these peptides can associate in vitro with the N-terminal or C-terminal domains of CAP fused to GST. Our observations indicate that CAP contains regions in both the N-terminal and C-terminal domains that are capable of interacting with each other or with themselves. Furthermore, we found that myc-epitope-tagged CAP coimmunoprecipitates with HA-epitope-tagged CAP from either yeast or mammalian cell extracts. Similar results demonstrate that human CAP can also interact with human CAP2. We also show that human CAP interacts with actin, both by the yeast two-hybrid test and by coimmunoprecipitation of epitope-tagged CAP from yeast or mammalian cell extracts. This interaction requires the C-terminal domain of CAP, but not the N-terminal domain. Thus CAP appears to be capable of interacting in vivo with other CAP molecules, CAP2, and actin. We also show that actin co-immunoprecipitates with HA-CAP2 from mammalian cell extracts.

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Association of Elongation Factor 1α and Ribosomal Protein L3 with the Proline-Rich Region of Yeast Adenylyl Cyclase-Associated Protein CAP

Yanagihara

Shinkai

Kariya

et al. 1997

Biochemical and Biophysical Research Communications

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Molecular cloning and characterization of a rat homolog of CAP, the adenylyl cyclase-associated protein from Saccharomyces cerevisiae

Cited by 41 publications

References 26 publications

Mammalian homolog of the yeast cyclase associated protein, CAP/Srv2p, regulates actin filament assembly

Mammalian homolog of the yeast cyclase associated protein, CAP/Srv2p, regulates actin filament assembly

Mammalian CAP interacts with CAP, CAP2, and actin

Association of Elongation Factor 1α and Ribosomal Protein L3 with the Proline-Rich Region of Yeast Adenylyl Cyclase-Associated Protein CAP

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