Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast

Li, Ge; Zhao, Yuqi

doi:10.1007/978-1-4939-7546-4_5

Cited by 9 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are a number of advantages of using a fission yeast cell-based system for future drug discovery and testing. First, fission yeast is a single eukaryotic organism that shares many highly conserved basic cell activities such as cell proliferation, cell cycle regulation, and regulation of cell death [ 71 , 72 , 73 ]. It is present mostly in its haploid state, which makes it easier to study the genetic effect of interest by avoiding high genetic complexity such as the dominance or recession of a genetic trait in human cells.…”

Section: Discussionmentioning

confidence: 99%

Single-Agent and Fixed-Dose Combination HIV-1 Protease Inhibitor Drugs in Fission Yeast (Schizosaccharomyces pombe)

Zhang

Vernon

et al. 2021

Pathogens

Self Cite

View full text Add to dashboard Cite

Successful combination antiretroviral therapies (cART) eliminate active replicating HIV-1, slow down disease progression, and prolong lives. However, cART effectiveness could be compromised by the emergence of viral multidrug resistance, suggesting the need for new drug discoveries. The objective of this study was to further demonstrate the utility of the fission yeast cell-based systems that we developed previously for the discovery and testing of HIV protease (PR) inhibitors (PIs) against wild-type or multi-PI drug resistant M11PR that we isolated from an infected individual. All thirteen FDA-approved single-agent and fixed-dose combination HIV PI drugs were tested. The effect of these drugs on HIV PR activities was tested in pure compounds or formulation drugs. All FDA-approved PI drugs, except for a prodrug FPV, were able to suppress the wild-type PR-induced cellular and enzymatic activities. Relative drug potencies measured by EC50 in fission yeast were discussed in comparison with those measured in human cells. In contrast, none of the FDA-approved drugs suppressed the multi-PI drug resistant M11PR activities. Results of this study show that fission yeast is a reliable cell-based system for the discovery and testing of HIV PIs and further demonstrate the need for new PI drugs against viral multi-PI resistance.

show abstract

Section: Discussionmentioning

confidence: 99%

Single-Agent and Fixed-Dose Combination HIV-1 Protease Inhibitor Drugs in Fission Yeast (Schizosaccharomyces pombe)

Zhang

Vernon

et al. 2021

Pathogens

Self Cite

View full text Add to dashboard Cite

show abstract

“…Example of such a fission yeast system is shown in Fig. 2 68 70 . Please note that there is nothing new in the molecular features of these shuttle vectors.…”

Section: Yeast For Understanding Virus-host Interactionsmentioning

confidence: 99%

“…It has also been used as a host system to study virus-host interactions including the effect of viral proteins on cell cycle regulation 63 64 , gene expression, cell death and apoptosis 65 66 67 . In addition, fission yeast has been used to carry out large-scale and functional characterization of small viral genomes such as human immunodeficiency virus type 1 (HIV-1) and Zika virus (ZIKV) 55 68 69 70 . General reviews on some of the related topics have been published previously 11 22 64 71 72 73 74 .…”

Section: Introductionmentioning

confidence: 99%

Yeast for virus research

Zhao¹

2017

Microb Cell

View full text Add to dashboard Cite

Budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe) are two popular model organisms for virus research. They are natural hosts for viruses as they carry their own indigenous viruses. Both yeasts have been used for studies of plant, animal and human viruses. Many positive sense (+) RNA viruses and some DNA viruses replicate with various levels in yeasts, thus allowing study of those viral activities during viral life cycle. Yeasts are single cell eukaryotic organisms. Hence, many of the fundamental cellular functions such as cell cycle regulation or programed cell death are highly conserved from yeasts to higher eukaryotes. Therefore, they are particularly suited to study the impact of those viral activities on related cellular activities during virus-host interactions. Yeasts present many unique advantages in virus research over high eukaryotes. Yeast cells are easy to maintain in the laboratory with relative short doubling time. They are non-biohazardous, genetically amendable with small genomes that permit genome-wide analysis of virologic and cellular functions. In this review, similarities and differences of these two yeasts are described. Studies of virologic activities such as viral translation, viral replication and genome-wide study of virus-cell interactions in yeasts are highlighted. Impacts of viral proteins on basic cellular functions such as cell cycle regulation and programed cell death are discussed. Potential applications of using yeasts as hosts to carry out functional analysis of small viral genome and to develop high throughput drug screening platform for the discovery of antiviral drugs are presented.

show abstract

“…The ease of culturing, short doubling time, availability of a wealth of experimental tools for genetic manipulation, and possibility of heterologous expression of proteins of higher eukaryotes are some other advantages that make yeast an important model system for viral studies. Two commonly used yeast models for such studies are Saccharomyces cerevisiae (budding yeast) and Schizosaccharomyces pombe (fission yeast) (Zhao 2017;Li and Zhao 2018). Additionally, other yeast models such as Pichia pastoris and Hansenula polymorpha are also extensively used to express viral proteins for the production of vaccines (Vogl et al 2013).…”

Section: Yeast: Apt But With Cautionmentioning

confidence: 99%

Contribution of yeast models to virus research

Glingston

Yadav

Rajpoot

et al. 2021

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Time and again, yeast has proven to be a vital model system to understand various crucial basic biology questions. Studies related to viruses are no exception to this. This simple eukaryotic organism is an invaluable model for studying fundamental cellular processes altered in the host cell due to viral infection or expression of viral proteins. Mechanisms of infection of several RNA and relatively few DNA viruses have been studied in yeast to date. Yeast is used for studying several aspects related to the replication of a virus, such as localization of viral proteins, interaction with host proteins, cellular effects on the host, etc. The development of novel techniques based on high-throughput analysis of libraries, availability of toolboxes for genetic manipulation, and a compact genome makes yeast a good choice for such studies. In this review, we provide an overview of the studies that have used yeast as a model system and have advanced our understanding of several important viruses. Key points• Yeast, a simple eukaryote, is an important model organism for studies related to viruses.• Several aspects of both DNA and RNA viruses of plants and animals are investigated using the yeast model.• Apart from the insights obtained on virus biology, yeast is also extensively used for antiviral development.

show abstract

Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast

Cited by 9 publications

References 26 publications

Single-Agent and Fixed-Dose Combination HIV-1 Protease Inhibitor Drugs in Fission Yeast (Schizosaccharomyces pombe)

Single-Agent and Fixed-Dose Combination HIV-1 Protease Inhibitor Drugs in Fission Yeast (Schizosaccharomyces pombe)

Yeast for virus research

Contribution of yeast models to virus research

Contact Info

Product

Resources

About