Molecular cloning and characterization of the genes encoding an auxin efflux carrier and the auxin influx carriers associated with the adventitious root formation in mango (Mangifera indica L.) cotyledon segments

Li, Yunhe; Zou, Minghong; Feng, Bihong; Huang, Xia; Zhang, Zhi; Sun, Guangming

doi:10.1016/j.plaphy.2012.03.012

Cited by 31 publications

(25 citation statements)

References 34 publications

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“…23 Mutants or treatments resulting in disturbed polar auxin transport show reduced AR formation. 37,38 While auxins and auxin signaling are essential for all stages of LR development, 39-42 exogenous auxin stimulates during the first stage and inhibits later developmental stages of AR formation, [43][44][45] thus indicating different sensitivity of both root-types to exogenous auxin. Besides exogenous or newly biosynthesized auxin, auxin transport, through the ABC-type multidrug-resistance ABCB19 transporter, is also essential for AR induction in hypocotyls.…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide mediates strigolactone signaling in auxin and ethylene-sensitive lateral root formation in sunflower seedlings

Bharti

Bhatla²

2015

Plant Signaling & Behavior

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Strigolactones (SLs) play significant role in shaping root architecture whereby auxin-SL crosstalk has been observed in SL-mediated responses of primary root elongation, lateral root formation and adventitious root (AR) initiation. Whereas GR24 (a synthetic strigolactone) inhibits LR and AR formation, the effect of SL biosynthesis inhibitor (fluridone) is just the opposite (root proliferation). Naphthylphthalamic acid (NPA) leads to LR proliferation but completely inhibits AR development. The diffusive distribution of PIN1 in the provascular cells in the differentiating zone of the roots in response to GR24, fluridone or NPA treatments further indicates the involvement of localized auxin accumulation in LR development responses. Inhibition of LR formation by GR24 treatment coincides with inhibition of ACC synthase activity. Profuse LR development by fluridone and NPA treatments correlates with enhanced [Ca 2C ] cyt in the apical region and differentiating zones of LR, indicating a critical role of [Ca 2C ] in LR development in response to the coordinated action of auxins, ethylene and SLs. Significant enhancement of carotenoid cleavage dioxygenase (CCD) activity (enzyme responsible for SL biosynthesis) in tissue homogenates in presence of cPTIO (NO scavenger) indicates the role of endogenous NO as a negative modulator of CCD activity. Differences in the spatial distribution of NO in the primary and lateral roots further highlight the involvement of NO in SL-modulated root morphogenesis in sunflower seedlings. Present work provides new report on the negative modulation of SL biosynthesis through modulation of CCD activity by endogenous nitric oxide during SL-modulated LR development.

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Section: Introductionmentioning

confidence: 99%

Nitric oxide mediates strigolactone signaling in auxin and ethylene-sensitive lateral root formation in sunflower seedlings

Bharti

Bhatla²

2015

Plant Signaling & Behavior

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“…Additionally, lateral root development has been shown to depend on complex changes in expression of PIN proteins prior to lateral root initiation and in developing primordia (Benková et al, 2003;Laskowski et al, 2008;Lewis et al, 2011). A recent report describes increased auxin induction of auxin influx carriers in mango cotyledons induced to form adventitious roots (Li et al, 2012). These results indicate that a distinct set of auxin transport proteins control lateral root development and suggest that similar specificity and complexity might regulate adventitious root development.…”

mentioning

confidence: 96%

“…Formation of adventitious roots on stem segments is widely exploited for clonal propagation of important horticultural, crop, and forest species. Hormonal and wounding controls of this process have been described (De Klerk et al, 1999;Abarca and Díaz-Sala, 2009;Li et al, 2009), with the role of auxin transport in adventitious rooting being described in a diversity of species, including pine (Pinus taeda; Diaz-Sala et al, 1996;Hutchison et al, 1999), carnation (Dianthus caryophyluss; Garrido et al, 2002), and mango (Mangifera indica; Li et al, 2012). The molecular mechanisms by which root excision and auxin induce adventitious root formation are not yet clear and would be facilitated by studies in a genetically tractable model species.…”

mentioning

confidence: 99%

Localized Induction of the ATP-Binding Cassette B19 Auxin Transporter Enhances Adventitious Root Formation in Arabidopsis

2013

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Adventitious roots emerge from aerial plant tissues, and the induction of these roots is essential for clonal propagation of agriculturally important plant species. This process has received extensive study in horticultural species but much less focus in genetically tractable model species. We have explored the role of auxin transport in this process in Arabidopsis (Arabidopsis thaliana) seedlings in which adventitious root initiation was induced by excising roots from low-light-grown hypocotyls. Inhibition of auxin transport from the shoot apex abolishes adventitious root formation under these conditions. Root excision was accompanied by a rapid increase in radioactive indole-3-acetic acid (IAA) transport and its accumulation in the hypocotyl above the point of excision where adventitious roots emerge. Local increases in auxin-responsive gene expression were also observed above the site of excision using three auxin-responsive reporters. These changes in auxin accumulation preceded cell division events, monitored by a cyclin B1 reporter (pCYCB1;1:GUS), and adventitious root initiation. We examined excisioninduced adventitious root formation in auxin influx and efflux mutants, including auxin insensitive1, pin-formed1 (pin1), pin2, pin3, and pin7, with the most profound reductions observed in ATP-binding cassette B19 (ABCB19). An ABCB19 overexpression line forms more adventitious roots than the wild type in intact seedlings. Examination of transcriptional and translational fusions between ABCB19 and green fluorescent protein indicates that excision locally induced the accumulation of ABCB19 transcript and protein that is temporally and spatially linked to local IAA accumulation leading to adventitious root formation. These experiments are consistent with localized synthesis of ABCB19 protein after hypocotyl excision leads to enhanced IAA transport and local IAA accumulation driving adventitious root formation.

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“…2). IBA treatment in mango cotyledons also led to downregulation of PIN1at some points during adventitious rooting [45]. Similarly, in Sorghum bicolor, PIN1and PIN5 were downregulated by IAA treatment [47].…”

Section: Exogenous Auxin Induces Gene Expression Modifications In Whomentioning

confidence: 92%

“…2). In mango cotyledon segments, the expression of four members of the AUX family was increased in IBA-treated samples [45] but in Medicago truncatula, the expression of auxin carriers varied after treatment with IAA, and some members were up-while others were downregulated [46]. On the other hand, the exogenous auxin treatment led to an apparent decrease in AUX1levels at early induction phase in E. globulus and a significant decrease of both auxin transporters during formation phase in E. grandis (Fig.…”

Section: Exogenous Auxin Induces Gene Expression Modifications In Whomentioning

confidence: 97%

Comparative transcriptional analysis provides new insights into the molecular basis of adventitious rooting recalcitrance in Eucalyptus

et al. 2015

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Molecular cloning and characterization of the genes encoding an auxin efflux carrier and the auxin influx carriers associated with the adventitious root formation in mango (Mangifera indica L.) cotyledon segments

Cited by 31 publications

References 34 publications

Nitric oxide mediates strigolactone signaling in auxin and ethylene-sensitive lateral root formation in sunflower seedlings

Nitric oxide mediates strigolactone signaling in auxin and ethylene-sensitive lateral root formation in sunflower seedlings

Localized Induction of the ATP-Binding Cassette B19 Auxin Transporter Enhances Adventitious Root Formation in Arabidopsis

Comparative transcriptional analysis provides new insights into the molecular basis of adventitious rooting recalcitrance in Eucalyptus

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