Molecular cloning and characterization of S-adenosylmethionine decarboxylase gene in rubber tree (Hevea brasiliensis)

Abstract

S-Adenosylmethionine decarboxylase (SAMDC) is a key rate-limiting enzyme involved in polyamines biosynthesis, and it plays important roles in plant growth, development and stresses response. However, no SAMDC gene was reported in rubber tree. Here we report characteristics of an SAMDC gene (HbSAMDC1) in rubber tree. HbSAMDC1 contains a 1080 bp open reading frame (ORF) encoding 359 amino acids. Quantitative real-time PCR analyses revealed that HbSAMDC1 exhibited distinct expression patterns in different tissues and was regulated by various stresses, including drought, cold, salt, wounding, and H 2 O 2 treatments. HbSAMDC1 5 0 untranslated region (UTR) contains a highly conserved overlapping tiny and small upstream ORFs (uORFs), encoding 2 and 52 amino acid residues, respectively. No introns were located in the main ORF of HbSAMDC1, whereas two introns were found in the 5 0 UTR. In transgenic tobaccos, the highly conserved small uORF of HbSAMDC1 is found to be responsible for translational repression of downstream bglucuronidase reporter. To our knowledge, this is the first report on molecular cloning, expression profiles, and 5 0 UTR characteristics of HbSAMDC1. These results lay solid foundation for further elucidating HbSAMDC1 function in rubber tree.

SAMDC3 enhances resistance to Barley stripe mosaic virus by promoting the ubiquitination and proteasomal degradation of viral γb protein

“…We identified plant SAMDC3 as a direct interactor of γb in a Y2H screen and discovered that it positively regulates γb ubiquitination. SAMDC3 contained a conserved proenzyme‐processing site and a PEST domain that is associated with protein degradation (Reverte et al ., 2001; Zhao et al ., 2017). Indeed, both mammalian and Arabidopsis SAMDC undergo rapid turnover via degradation by the 26S proteasome after polyubiquitination (Pegg, 2009; Zhang et al ., 2011).…”

Enhancing Salt Tolerance of Plants: From Metabolic Reprogramming to Exogenous Chemical Treatments and Molecular Approaches

“…S-adenosylmethionine decarboxylase (SAMDC) is a key enzyme in PA biosynthesis, and it plays an important role in plant responses to different ESs [ 165 ]. Ectopic expression of SAMDC gene of Tritordeum in rice increased the levels of Spd and Spm and enhanced SS tolerance of transgenic plants [ 151 ].…”

“…cv Jiro) was transformed with a gene from apple encoding sorbitol-6-phosphate dehydrogenase displayed enhanced SS by accumulation of sorbitol in transgenic plants [159]. S-adenosylmethionine decarboxylase (SAMDC) is a key enzyme in PA biosynthesis, and it plays an important role in plant responses to different ESs [165]. Ectopic expression of SAMDC gene of Tritordeum in rice increased the levels of Spd and Spm and enhanced SS tolerance of transgenic plants [151].…”

Optimization Of The Annealing Temperature With Degenerate Primer For Amplification Of Arginine Decarboxylase (ADC) Fragment Gene From Genomic DNA of Maluku Tenggara Local Cassava

“…Runutan lengkap basa nukleotida dari genome tanaman Arabidopsis telah dipakai sebagai sarana/alat menggunakan pendekatan 'omic' untuk mengidentifikasi gen target yang terlibat dalam jalur sinyal dan biosintesis poliamin (Alcázar et al 2010). Peremarti et al (2010) telah melakukan cloning gen ADC dari tanaman padi, Zhao et al (2017), melakukan cloning gen SAMDC dari tanaman karet (Hevea brasiliensis), namun demikian, informasi mengenai runutan basa nukleotida dan fungsi dari gen ADC pada tanaman ubi kayu masih belum banyak dilakukan. Oleh karena itu sebagai tahap awal, telah dilakukan desain primer untuk memperoleh runutan basa nukleotida pengkode enzim poliamin dari tanaman ubi kayu genotipe lokal Malra.…”

“…(Wang et al, 2011), 55 o C pada tanaman padi (Peremarti etal., 2010), dan 58 o C pada Hevea brasiliensis (Zhao et al, 2017). Belum diperolehnya pita amplifikasi yang spesifik dan tajam menggunakan keempat pasang primer diduga karena suhu annealing yang digunakan masih terlalu rendah sehinggga banyak pita amplifikasi yang dihasilkan.…”