Molecular cloning and characterization of a novel bovine IFN-ε

Guo, Yongli; Gao, Mingchun; Bao, Jun; Luo, Xiuxin; Liu, Ying; An, Dong Sung; Zhang, Haili; Ma, Bo; Wang, Junwei

doi:10.1016/j.gene.2014.12.031

Cited by 17 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Three-dimensional structure prediction of PcIFN-α revealed that five alpha helices were consistent with the IFN molecules analyzed by nuclear magnetic resonance spectroscopy (Ghasriani et al 2013) (Fig. 1c), such as bovine IFN-ε (Guo et al 2015), Amur tigers IFN-α (Zhao et al 2017), and Whooper swan IFN-α (Zhao et al 2018). Moreover, the investigation of secondary structure also indicated that PcIFN-α has five putative alpha helices which were labeled as A to E (Fig.…”

Section: Characterization Of Pcifn-α Genesupporting

confidence: 59%

Molecular cloning and transcriptional regulation of Indian peafowl (Pavo cristatus) IFN-α gene

Wang

Zhao

Liu

et al. 2019

Cell Stress and Chaperones

View full text Add to dashboard Cite

Interferon-α (IFN-α) resists viral infections by triggering the transcription of a diverse range of antiviral IFN-stimulated genes (ISGs). However, information about the Indian peafowl (Pavo cristatus) IFN-α (PcIFN-α) has not been reported. In this study, a PcIFN-α gene was amplified, which encoded a protein of 193 amino acids with a 26-amino acid signal peptide sharing 72.16-95.70% identity with other avians in Aves. After expression in prokaryote, PcIFN-α was analyzed for its physicochemical property and antiviral activity. Intriguingly, compared with chicken IFN-α, an effective viral infection therapeutic agent, PcIFN-α showed superior anti-VSV, NDV, and AIV activities, which were then abrogated by rabbit anti-PcIFN-α antibodies in vitro. Moreover, PcIFN-α was shown to be highly sensitive to trypsin; however, it remained stable despite changes in pH and temperature. Additionally, PcIFN-α induced the transcriptional or translational levels of Mx1 and ISG12 genes time-dependently. Overall, the present study revealed that PcIFN-α is a potential novel effective therapeutic agent in antiviral defense responses in peafowl, improving understanding of its involvement in bird antiviral defense.

show abstract

Section: Characterization Of Pcifn-α Genesupporting

confidence: 59%

Molecular cloning and transcriptional regulation of Indian peafowl (Pavo cristatus) IFN-α gene

Wang

Zhao

Liu

et al. 2019

Cell Stress and Chaperones

View full text Add to dashboard Cite

show abstract

“…First, the antiviral, natural killer cell-cytotoxicity activity and antiproliferative activities of IFN-ε are weaker than IFN-α and IFN-β [27]. Second, some studies showed that IFN-ε exhibits antiviral activity against cells derived from species that have near relatives and are expected to be homologous cells [23, 25]. Third, IFN-ε differs from IFN-α in macrophages by inducing an antiviral state mediated by more different factors [4].…”

Section: Biological Activities Of Type I Ifnsmentioning

confidence: 99%

“…However, unlike IFN-α, it has cross-species activity to some extent. This activity indicates that cells have a tendency to be insensitive to IFN-ω from distantly related species [23]. IFN-ω is involved in the nonspecific response based on increased expression of several acute phase proteins and MHC I molecules; upregulation of the phagocytic activities of whole blood cells, macrophages and NK cell activities; and decreased concurrent viral excretion [71-74].…”

Section: Biological Activities Of Type I Ifnsmentioning

confidence: 99%

Type I Interferons: Distinct Biological Activities and Current Applications for Viral Infection

Gong

Zhao

et al. 2018

Cell Physiol Biochem

139

111

View full text Add to dashboard Cite

The interferons (IFNs) are a primary defense against pathogens because of the strong antiviral activities they induce. IFNs can be classified into three groups: type I, type II and type III, according to their genetic, structural, and functional characteristics and their receptors on the cell surface. The type I IFNs are the largest group and include IFN-α, IFN-β, IFN-ε, IFN-ω, IFN-κ, IFN-δ, IFN-τ and IFN-ζ. The use of IFNs for the treatment of viral infectious diseases on their antiviral activity may become an important therapeutic option, for example, IFN-α is well known for the successful treatment of hepatitis B and C virus infections, and interest is increasing in the antiviral efficacy of other novel IFN classes and their potential applications. Therefore, in this review, we summarize the recent progress in the study of the biological activities of all the type I IFN classes and their potential applications in the treatment of infections with immunodeficiency virus, hepatitis viruses, and influenza viruses.

show abstract

“…Antiviral activity was determined with a standard cytopathic effect assay (Rubinstein et al, 1981), which is similar to the previously described method (Guo et al, 2015;Rodriguez et al, 1998). Briefly, the monolayers of MDBK, MDCK, PK-15, BHK-21, or BT cells were seeded in 96-well plates, treated with 100 L of fourfold serial dilutions of BoIFN-24 for 24 h, and challenged with VSV (100TCID 50 /well).…”

Section: Antiviral Activity Of Boifn-24 In Vitromentioning

confidence: 99%