Molecular Cloning and Characterization of an Ortholog of NPR1 Gene from Dongguan Dajiao (Musa spp. ABB)

Abstract

The nonexpresser of pathogenesis-related gene 1 (NPR1) plays a pivotal role in systemic acquired resistance in plants. In this study, a novel full-length NPR1-like gene, designated MdNPR1 (accession number FJ357442), was isolated by RACE-PCR from cv. Dongguan Dajiao (Musa spp. ABB), a local banana cultivar known to be resistant to Fusarium oxysporum fsp. cubense (FOC) race 4. Sequence alignment showed that MdNPR1 contained an ankyrin repeat domain and a broad complex, tramtrack, and brica-brac (BTB) domain. Semiquantitative reverse transcription polymerase chain reaction revealed that MdNPR1 could be constitutively expressed at low levels in both of the FOC race 4-susceptible cultivar Fenjiao (Musa spp. ABB) and the resistant cultivar Dongguan Dajiao. However, MdNPR1 could be induced by exogenous application of salicylic acid in cv. Dongguan Dajiao, but not in cv. Fenjiao. Moreover, the accumulated level of MdNPR1 transcripts in cv. Dongguan Dajiao was higher than that in cv. Fenjiao when plants were treated with FOC race 4 inoculation. Our results implied that MdNPR1 might represent as a promising candidate for engineering resistant to broad-spectrum pathogen in banana.

Two Different Banana NPR1-Like Coding Sequences Confer Similar Protection Against Pathogens in Arabidopsis

“…(MdNPR1; Zhao et al, 2009). Characterization of these banana NPR1-like genes has been limited to transcription analysis without any direct proof of activity.…”

Evaluation of High-Resolution Melting for Gene Mapping in Rice

“…However, diseases, such as bacterial blight, have caused severe reductions in rice yield (Zhao et al 2010). To improve resistance and raise the yield, great efforts have been made to clone resistance genes in rice and other plants (Feng et al 2009;Zhao et al 2009;Leng et al 2010). Many resistance genes have been cloned and now contribute to improve disease resistance; one example is gene Xa21, which confers resistance to almost all Indian and Philippine races of Xanthomonas oryzae pv.…”

Xanthomonas vasicola pv. musacearum down-regulates selected defense genes during its interaction with both resistant and susceptible banana

“…Indeed this is what was exactly observed in this study where MdNPR1 expression pattern was consistent with PR3 expression pattern. MdNPR1 expression has already been shown to be induced by an array of elicitors including SA and FOC treatment in some banana cultivars [18]. Since NPR1 is normally induced in response to different abiotic and biotic stress factors, it would also be expected to be induced by Xvm.…”