Molecular cloning and characterization of the nitric oxide synthase gene from kuruma shrimp, Marsupenaeus japonicus

Molecular cloning and expression profiles of nitric oxide synthase (NOS) in mud crab Scylla paramamosain

“…1) and codes for a 1124 amino acid protein with an estimated theoretical mass of 137.3 kDa and pI of 6.56. These findings agree closely with previous results from studies of the land crab G. lateralis [7], which reported a 136 kDa NOS, and the kuruma shrimp, a 134 kDa NOS immuno-reactive protein found in eyestalk neural ganglia extract [9]. Structural analysis of the SpNOS amino acid sequence by SMART program revealed a highly conserved NO synthase domain homolog starting at position 51 and ending at position 422 at the Nterminal region, a flavodoxin 1 domain starting at position 467 and ending at position 673, a FAD-binding-1 domain at position 727-961 and a typical NAD-binding-1 domain homolog at position 993-1107 at the C-terminal region (Fig.…”

“…Some reports have confirmed the presence of NOS activity in some crustaceans, and NOS sequence have been cloned and characterized in crab [7,8] shrimp [9,10], and lobster [11]. However, the sequence of NOS in mud crab usually cultured in brackish water in southern China, S. paramamosain is kept unknown.…”

Involvement of nitric oxide in the in vitro interaction between Manila clam, Ruditapes philippinarum, hemocytes and the bacterium Vibrio tapetis

“…In spite of the large number of studies concerning NO production, the NOS gene has been identified in only a few species of invertebrates including insects, two shrimp species [33,34], one snail [35], and one mollusk [36]. A NOS-like gene in the oyster Crassostrea virginica has been isolated [37] but the gene in R. philippinarum has not yet been identified.…”

Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase

“…And McDonald et al (2011) found high expression of NOS in Y-organs of the green shore crab Carcinus maenas. In the Pacific white shrimp L. vannamei and the mud crab S. paramamosain, expression has been detected in the hepatopancreas (Yao et al, 2010;Li et al, 2012), whereas in the kuruma shrimp M. japonicus, it has been observed in the gills and brain (Inada et al, 2010). In addition, iNOS is expressed in hemocytes of the spiny lobster Panulirus argus (Rodríguez-Ramos et al, 2010).…”