Molecular cloning and characterization of the protein 4.1O gene, a novel member of the protein 4.1 family with focal expression in ovary

Ni, Xiaohua; Ji, Chaoneng; Cao, Gentao; Cheng, Haipeng; Guo, Li; Gu, Shaohua; Kong, Ying; Zhao, Robert Chunhua; Mao, Yumin

doi:10.1007/s100380300015

Cited by 22 publications

(24 citation statements)

References 10 publications

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“…MGC20553 is a multifunctional protein essential for maintaining erythrocyte shape and membrane mechanical properties (37). The exact function of this gene in blood cells has yet to be determined.…”

Section: Discussionmentioning

confidence: 99%

Novel Blood-Based, Five-Gene Biomarker Set for the Detection of Colorectal Cancer

Han¹,

Liew

Zhang³

et al. 2008

Clinical Cancer Research

113

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Purpose: We applied a unique method to identify genes expressed in whole blood that can serve as biomarkers to detect colorectal cancer (CRC). Experimental Design:Total RNA was isolated from 211blood samples (110 non-CRC,101CRC). Microarray and quantitative real-time PCR were used for biomarker screening and validation, respectively. Results: From a set of 31 RNA samples (16 CRC, 15 controls), we selected 37 genes from analyzed microarray data that differed significantly between CRC samples and controls (P < 0.05).We tested these genes with a second set of 115 samples (58 CRC, 57 controls) using quantitative real-time PCR, validating 17 genes as differentially expressed. Five of these genes were selected for logistic regression analysis, of which two were the most up-regulated (CDA and MGC20553) and three were the most down-regulated (BANK1, BCNP1, and MS4A1) in CRC patients. Logit

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Section: Discussionmentioning

confidence: 99%

Novel Blood-Based, Five-Gene Biomarker Set for the Detection of Colorectal Cancer

Han¹,

Liew

Zhang³

et al. 2008

Clinical Cancer Research

113

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“…55 FERM3 encodes a structural protein of unknown function, protein 4.1O, which belongs to the 4.1 family of cytoskeletal proteins. 56 External validation of both loci was performed in a cohort of 1,304 participants of the Diabetes Control and Complications Trial (DCCT)/Epidemiology of Diabetes Interventions and Complications (EDIC) study. 55 …”

Section: Gwass Of Kidney Disease Phenotypesmentioning

confidence: 99%

Genome-wide association studies of chronic kidney disease: what have we learned?

O’Seaghdha

Fox

2011

Nat Rev Nephrol

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The past 3 years have witnessed a dramatic expansion in our knowledge of the genetic determinants of estimated glomerular filtration rate (eGFR) and chronic kidney disease (CKD). However, heritability estimates of eGFR indicate that we have only identified a small proportion of the total heritable contribution to the phenotypic variation. The majority of associations reported from genome-wide association studies identify genomic regions of interest and further work will be required to identify the causal variants responsible for a specific phenotype. Progress in this area is likely to stem from the identification of novel risk genotypes, which will offer insight into the pathogenesis of disease and potential novel therapeutic targets. Follow-up studies stimulated by findings from genome-wide association studies of kidney disease are already yielding promising results, such as the identification of an association between urinary uromodulin levels and incident CKD. Although this work is at an early stage, prospects for progress in our understanding of CKD and its treatment look more promising now than at any point in the past.

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“…The first member to be discovered, 4.1R, was shown to be essential for maintaining normal cell shape by connecting plasma membrane proteins to the spectrin-actin cytoskeleton in erythrocytes (Tyler et al, 1979;Ungewickell et al, 1979). To date, four additional 4.1 family members have been described: 4.1G [general Walensky et al, 1998)], 4.1N [neural (Walensky et al, 1999)], 4.1B [brain (Parra et al, 2000)] and 4.1O [ovary (Ni et al, 2003)]. All members of the 4.1 family share the membrane-binding (FERM) domain, the spectrin-actinbinding (SAB) domain, the C-terminal domain (CTD), and nonconserved regions at the N-terminus (U1) and between domains (U2, U3).…”

Section: Introductionmentioning

confidence: 99%

Interaction of 4.1G and cGMP-gated channels in rod photoreceptor outer segments

Cheng

Molday

2013

Journal of Cell Science

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SummaryIn photoreceptors, the assembly of signaling molecules into macromolecular complexes is important for phototransduction and maintaining the structural integrity of rod outer segments (ROSs). However, the molecular composition and formation of these complexes are poorly understood. Using immunoprecipitation and mass spectrometry, 4.1G was identified as a new interacting partner for the cyclic-nucleotide gated (CNG) channels in ROSs. 4.1G is a widely expressed multifunctional protein that plays a role in the assembly and stability of membrane protein complexes. Multiple splice variants of 4.1G were cloned from bovine retina. A smaller splice variant of 4.1G selectively interacted with CNG channels not associated with peripherin-2-CNG channel complex. A combination of truncation studies and domain-binding assays demonstrated that CNG channels selectively interacted with 4.1G through their FERM and CTD domains. Using immunofluorescence, labeling of 4.1G was seen to be punctate and partially colocalized with CNG channels in the ROS. Our studies indicate that 4.1G interacts with a subset of CNG channels in the ROS and implicate this protein-protein interaction in organizing the spatial arrangement of CNG channels in the plasma membrane of outer segments.

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Molecular cloning and characterization of the protein 4.1O gene, a novel member of the protein 4.1 family with focal expression in ovary

Cited by 22 publications

References 10 publications

Novel Blood-Based, Five-Gene Biomarker Set for the Detection of Colorectal Cancer

Novel Blood-Based, Five-Gene Biomarker Set for the Detection of Colorectal Cancer

Genome-wide association studies of chronic kidney disease: what have we learned?

Interaction of 4.1G and cGMP-gated channels in rod photoreceptor outer segments

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